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- PDB-5v7s: Crystal structure of Influenza A virus matrix protein M1 (NLS-88E... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5v7s | |||||||||
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Title | Crystal structure of Influenza A virus matrix protein M1 (NLS-88E, pH 6.2) | |||||||||
![]() | Matrix protein 1 | |||||||||
![]() | VIRAL PROTEIN / Influenza A / Matrix protein / NLS-88E mutant / pH 6.2 | |||||||||
Function / homology | ![]() viral budding from plasma membrane / structural constituent of virion / host cell nucleus / virion membrane / RNA binding / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Musayev, F.N. / Safo, M.K. / Althufairi, B. / Desai, U.R. / Xie, H. / Mosier, P.D. / Chiang, M.-J. / Zhou, Q. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Maintaining pH-dependent conformational flexibility of M1 is critical for efficient influenza A virus replication. Authors: Chiang, M.J. / Musayev, F.N. / Kosikova, M. / Lin, Z. / Gao, Y. / Mosier, P.D. / Althufairi, B. / Ye, Z. / Zhou, Q. / Desai, U.R. / Xie, H. / Safo, M.K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 102.6 KB | Display | ![]() |
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PDB format | ![]() | 77.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 458.5 KB | Display | ![]() |
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Full document | ![]() | 469.5 KB | Display | |
Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 25.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5v6gC ![]() 5v7bC ![]() 5v8aC ![]() 1ea3S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19069.975 Da / Num. of mol.: 3 / Mutation: G88E, R101S, R105S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: A/Wilson-Smith/1933 H1N1 / Plasmid: pET30a / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.2 Details: Protein solution: 8 mg/ml in 50mM K2HPO4/KH2PO4/H3PO4, 0.2M NaCl, 10mM bME, pH 3.2 Reservoir solution: 0.1M Tris-HCl, pH 8.2, 8% PEG-8K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Aug 20, 2015 / Details: Rigaku varimax confocal |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→26.23 Å / Num. obs: 14742 / % possible obs: 90.7 % / Redundancy: 5.37 % / Biso Wilson estimate: 55.06 Å2 / Rmerge(I) obs: 0.077 / Net I/σ(I): 12.1 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 5.51 % / Rmerge(I) obs: 0.449 / Mean I/σ(I) obs: 3.6 / Num. unique obs: 1473 / % possible all: 91.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1EA3 Resolution: 2.5→26.23 Å / SU ML: 0.49 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 35.93
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→26.23 Å
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Refine LS restraints |
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LS refinement shell |
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