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- PDB-5upb: Swit_4259, an Acetoacetate Decarboxylase-like Enzyme from Sphingo... -

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Basic information

Entry
Database: PDB / ID: 5upb
TitleSwit_4259, an Acetoacetate Decarboxylase-like Enzyme from Sphingomonas wittichii RW1
ComponentsAcetoacetate decarboxylase
KeywordsLYASE / Acetoacetate Decarboxylase-like Enzyme
Function / homologyAcetoacetate decarboxylase / Acetoacetate decarboxylase domain superfamily / Acetoacetate decarboxylase (ADC) / carboxy-lyase activity / Acetoacetate decarboxylase
Function and homology information
Biological speciesSphingomonas wittichii (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.912 Å
AuthorsMydy, L.S. / Silvaggi, N.R.
Funding support United States, 1items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)MCB-1157392 United States
CitationJournal: Acta Crystallogr F Struct Biol Commun / Year: 2017
Title: Swit_4259, an acetoacetate decarboxylase-like enzyme from Sphingomonas wittichii RW1.
Authors: Mydy, L.S. / Mashhadi, Z. / Knight, T.W. / Fenske, T. / Hagemann, T. / Hoppe, R.W. / Han, L. / Miller, T.R. / Schwabacher, A.W. / Silvaggi, N.R.
History
DepositionFeb 2, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 7, 2017Provider: repository / Type: Initial release
Revision 1.1Sep 13, 2017Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2Sep 27, 2017Group: Experimental preparation / Category: exptl_crystal_grow / Item: _exptl_crystal_grow.pdbx_details
Revision 1.3Dec 6, 2017Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.4Dec 13, 2017Group: Database references / Category: citation / citation_author
Item: _citation.journal_abbrev / _citation.pdbx_database_id_PubMed ..._citation.journal_abbrev / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.5Nov 27, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.6Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Acetoacetate decarboxylase
B: Acetoacetate decarboxylase
C: Acetoacetate decarboxylase
D: Acetoacetate decarboxylase


Theoretical massNumber of molelcules
Total (without water)110,8974
Polymers110,8974
Non-polymers00
Water7,710428
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19630 Å2
ΔGint-116 kcal/mol
Surface area32740 Å2
MethodPISA
Unit cell
Length a, b, c (Å)75.040, 75.040, 179.850
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number145
Space group name H-MP32
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 20 through 33 or (resid 34...
21(chain B and (resid 20 through 33 or (resid 34...
31(chain C and (resid 20 through 33 or (resid 34...
41(chain D and (resid 20 through 33 or (resid 34...

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ALAALALEULEU(chain A and (resid 20 through 33 or (resid 34...AA20 - 3319 - 32
12ARGARGARGARG(chain A and (resid 20 through 33 or (resid 34...AA3433
13ALAALALEULEU(chain A and (resid 20 through 33 or (resid 34...AA4 - 2593 - 258
14ALAALALEULEU(chain A and (resid 20 through 33 or (resid 34...AA4 - 2593 - 258
15ALAALALEULEU(chain A and (resid 20 through 33 or (resid 34...AA4 - 2593 - 258
16ALAALALEULEU(chain A and (resid 20 through 33 or (resid 34...AA4 - 2593 - 258
21ALAALALEULEU(chain B and (resid 20 through 33 or (resid 34...BB20 - 3319 - 32
22ARGARGARGARG(chain B and (resid 20 through 33 or (resid 34...BB3433
23ALAALALEULEU(chain B and (resid 20 through 33 or (resid 34...BB4 - 2593 - 258
24ALAALALEULEU(chain B and (resid 20 through 33 or (resid 34...BB4 - 2593 - 258
25ALAALALEULEU(chain B and (resid 20 through 33 or (resid 34...BB4 - 2593 - 258
26ALAALALEULEU(chain B and (resid 20 through 33 or (resid 34...BB4 - 2593 - 258
31ALAALALEULEU(chain C and (resid 20 through 33 or (resid 34...CC20 - 3319 - 32
32ARGARGARGARG(chain C and (resid 20 through 33 or (resid 34...CC3433
33ALAALALEULEU(chain C and (resid 20 through 33 or (resid 34...CC2 - 2591 - 258
34ALAALALEULEU(chain C and (resid 20 through 33 or (resid 34...CC2 - 2591 - 258
35ALAALALEULEU(chain C and (resid 20 through 33 or (resid 34...CC2 - 2591 - 258
36ALAALALEULEU(chain C and (resid 20 through 33 or (resid 34...CC2 - 2591 - 258
41ALAALALEULEU(chain D and (resid 20 through 33 or (resid 34...DD20 - 3319 - 32
42ARGARGARGARG(chain D and (resid 20 through 33 or (resid 34...DD3433
43ALAALAGLUGLU(chain D and (resid 20 through 33 or (resid 34...DD2 - 2571 - 256
44ALAALAGLUGLU(chain D and (resid 20 through 33 or (resid 34...DD2 - 2571 - 256
45ALAALAGLUGLU(chain D and (resid 20 through 33 or (resid 34...DD2 - 2571 - 256
46ALAALAGLUGLU(chain D and (resid 20 through 33 or (resid 34...DD2 - 2571 - 256

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Components

#1: Protein
Acetoacetate decarboxylase


Mass: 27724.359 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sphingomonas wittichii (bacteria) / Strain: RW1 / DSM 6014 / JCM 10273 / Gene: Swit_4259 / Production host: Escherichia coli (E. coli) / References: UniProt: A5VE83
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 428 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.64 Å3/Da / Density % sol: 53.45 % / Description: Long, hexagonal rods
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8
Details: 12% PEG 10000, 0.1M Tris pH 8.0, 0.3M ammonium acetate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.97872 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Nov 22, 2011
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97872 Å / Relative weight: 1
ReflectionResolution: 1.91→44.96 Å / Num. obs: 87644 / % possible obs: 100 % / Redundancy: 5.5 % / CC1/2: 0.993 / Rmerge(I) obs: 0.106 / Net I/av σ(I): 6.8 / Net I/σ(I): 13.92
Reflection shellResolution: 1.91→1.95 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.412 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 4465 / CC1/2: 0.8 / % possible all: 99.8

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Processing

Software
NameVersionClassification
PHENIX1.11_2567refinement
MOSFLM7.0.7data reduction
Aimless0.1.27data scaling
PHASER2.5.1phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4ZBO, 4JM3

4zbo

4jm3


Resolution: 1.912→44.064 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 0.62 / Phase error: 23.01 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2011 5983 3.44 %
Rwork0.1716 167702 -
obs0.1726 173685 99.11 %
Solvent computationShrinkage radii: 0.5 Å / VDW probe radii: 0.9 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 398.65 Å2 / Biso mean: 53.1377 Å2 / Biso min: 20.65 Å2
Refinement stepCycle: final / Resolution: 1.912→44.064 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7752 0 0 428 8180
Biso mean---45.25 -
Num. residues----1021
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0117965
X-RAY DIFFRACTIONf_angle_d1.17610863
X-RAY DIFFRACTIONf_chiral_restr0.071178
X-RAY DIFFRACTIONf_plane_restr0.0081423
X-RAY DIFFRACTIONf_dihedral_angle_d11.3024595
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A5223X-RAY DIFFRACTION7.254TORSIONAL
12B5223X-RAY DIFFRACTION7.254TORSIONAL
13C5223X-RAY DIFFRACTION7.254TORSIONAL
14D5223X-RAY DIFFRACTION7.254TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.9115-1.97990.30715610.2737160601662195
1.9799-2.05910.27495930.2386167361732999
2.0591-2.15280.24866060.2154167991740599
2.1528-2.26630.22296150.1857168011741699
2.2663-2.40830.21175940.17441689717491100
2.4083-2.59420.22245780.1771689817476100
2.5942-2.85530.21966000.17771684417444100
2.8553-3.26830.19266190.17111694417563100
3.2683-4.11730.19626040.15991691017514100
4.1173-44.07590.16566130.1498168131742699
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.58270.0674-0.57471.01560.31832.09710.2004-0.47410.072-0.01970.0499-0.386-0.99040.63-0.30630.4958-0.1303-0.02530.4826-0.07090.52763.14313.66594.021
22.18610.107-0.0011.66080.90921.51920.02360.1511-0.01130.1307-0.20560.06380.03270.36370.16440.2591-0.0199-0.00240.35050.02750.2-4.2855-7.278920.5171
32.70250.979-1.57574.3232-1.75534.8309-0.2659-0.1961-0.34560.136-0.0155-0.76190.68570.33350.21970.40830.08790.04850.30010.02620.52489.0026-19.653214.6197
41.4439-1.39840.31061.7083-0.14081.61860.01640.10120.24670.3353-0.2584-0.2102-0.21290.16680.21710.2043-0.0608-0.03920.2396-0.0050.2845-5.94646.111715.4624
51.1369-0.2882-0.16830.76270.14531.2095-0.0143-0.208-0.07790.0925-0.053-0.11760.20150.10540.02410.239-0.0260.00530.2996-0.02090.2726-5.2937-5.078815.7454
60.83710.2724-0.39460.3293-0.04622.02180.025-0.1052-0.1058-0.0064-0.03530.04090.370.04760.10670.3283-0.03260.03620.2353-0.01610.3064-13.2729-12.946215.1726
70.98940.3247-0.49620.56390.31542.00460.0446-0.0008-0.05850.0298-0.0838-0.1657-0.08080.42690.07010.25110.05220.01480.3634-0.01020.30659.3049-5.557511.1397
80.6070.0561-0.05631.24890.36531.0999-0.1078-0.1407-0.15780.04290.1064-0.06460.28910.09830.00050.3252-0.01910.01280.26010.00370.2595-6.6147-7.798825.068
90.9057-0.4190.08450.20580.06530.89840.0149-0.0694-0.11140.0243-0.0079-0.10920.29680.2082-0.01130.3818-0.02280.02630.31530.00370.33860.6001-10.928115.2116
100.4249-0.4630.37890.76980.29291.83010.02560.1214-0.0729-0.0678-0.0452-0.20180.41960.46420.0180.3330.09590.04230.4769-0.03480.4005-0.0225-4.282-9.6955
116.7738-1.93372.4115.5694-2.26185.9544-0.38560.26370.9781-0.16770.1408-0.6817-1.12730.12710.05950.5614-0.0851-0.00460.40710.02570.46185.405221.2792-12.9985
121.02540.3482-0.22260.59280.36041.7951-0.06090.077-0.1752-0.1597-0.1072-0.36280.21630.16040.14330.28370.05290.05370.28090.01420.2806-4.0284-1.6725-13.4972
131.24840.0892-0.32860.77180.27951.4647-0.06660.25050.0412-0.14520.0274-0.1148-0.04050.08890.0150.30850.01190.02180.3238-0.01920.2667-7.54943.8848-13.7697
140.5925-0.01170.09180.22150.01751.2890.06530.1670.1342-0.1426-0.061-0.0167-0.1497-0.1883-0.01950.31120.0784-0.00330.3321-0.01120.2686-15.318411.3792-12.9879
151.2391-0.51361.26930.37370.05522.71590.0309-0.02160.0526-0.1473-0.0311-0.1581-0.0310.26690.10270.295-0.03570.04260.3959-0.00920.3288.21417.2761-9.2533
160.6071-0.3899-0.10491.21160.39880.8503-0.01560.22810.0629-0.0638-0.04060.0532-0.1067-0.13590.0360.35060.01890.00770.35870.01290.2632-8.29787.201-23.0565
170.57750.3603-0.0540.25960.10781.4975-0.0251-0.00130.2326-0.12520.0444-0.1012-0.34940.2236-0.01440.35860.00210.01520.3029-0.0050.3267-1.282111.4063-13.2138
180.83430.51580.19110.31390.01781.9655-0.0223-0.1548-0.09670.1335-0.1990.22310.3253-0.61570.15740.2678-0.09580.07320.5282-0.09950.3998-32.7415-1.319213.0891
192.74811.30781.40313.37591.19991.37180.0695-0.01820.3688-0.0322-0.05040.3712-0.3254-0.6699-0.02360.31880.2149-0.06850.7689-0.0070.6947-41.466821.58967.1318
201.6338-0.8115-0.14092.3454-0.95231.974-0.06-0.2244-0.1044-0.0277-0.03920.3398-0.0036-0.59590.03630.2694-0.03660.01920.4861-0.09080.3589-29.54033.219816.6486
211.6935-0.42260.21031.3528-0.34141.0580.056-0.0817-0.01070.027-0.12840.16320.0537-0.42390.09620.2209-0.04360.00110.4274-0.08650.283-28.90080.990114.2318
220.85260.2639-0.12390.6582-0.40430.2462-0.0732-0.10370.2580.11680.01110.28550.0032-0.58670.03710.24150.0130.01410.5759-0.09930.3667-32.41376.08419.5877
232.0456-2.28010.59742.7761-0.69950.1959-0.1696-0.19880.39820.45950.0812-0.4101-0.4428-0.07020.05710.3844-0.0549-0.0630.3708-0.07020.37-8.98511.885225.1762
241.2026-0.16460.05090.82520.14821.22410.0848-0.10750.20580.1585-0.0962-0.0535-0.306-0.35710.00440.27490.0196-0.01620.3368-0.05990.2791-19.678415.292710.0228
251.3933-0.03890.73330.1652-0.33691.31130.0938-0.3557-0.0562-0.02410.23930.0156-0.2672-0.6341-0.04780.29980.08150.05140.8728-0.14310.4227-37.826312.012916.1376
260.3222-0.18940.07291.42611.79426.54910.00940.27610.1933-0.02010.10090.15130.4367-0.7126-0.05530.3288-0.0654-0.04391.0812-0.08620.4374-47.43221.36240.0623
271.939-0.13970.05121.4201-0.39592.2579-0.0995-0.28840.23630.4084-0.01870.2338-0.2731-0.68540.11050.2880.04290.03790.4624-0.12230.3624-26.293113.774721.1733
280.8704-0.39990.45350.1936-0.14441.19610.02640.00250.06890.0347-0.0660.0381-0.1587-0.43-00.28830.05360.00480.5203-0.09250.376-33.482813.404210.757
290.43160.11560.57930.33661.00963.1349-0.12910.37180.111-0.35490.20210.1306-0.4802-1.08480.00170.35530.0627-0.03680.8351-0.02450.4346-38.09419.0366-6.3684
301.47680.7931-0.47792.0064-0.00210.9141-0.07640.2381-0.1261-0.2416-0.10570.4460.6108-0.89190.00360.4883-0.30020.03390.6761-0.18140.3919-32.0396-16.2303-10.7452
311.38470.5809-0.33011.8184-0.09820.7961-0.00120.2949-0.0582-0.1772-0.15120.14710.2355-0.67120.04660.343-0.0718-0.00850.5314-0.12420.2719-27.7917-3.511-11.6744
320.2619-0.47420.19720.7972-0.36490.1651-0.08520.2816-0.2475-0.0929-0.07720.22780.423-0.69230.11610.3328-0.16050.02790.5803-0.14670.3151-29.4483-9.606-8.4334
330.7877-0.12730.54491.20030.02770.55880.010.1448-0.2327-0.2673-0.1989-0.06490.8004-0.15620.16260.5662-0.06540.09550.3116-0.08870.3528-14.5705-16.7195-12.4317
340.4439-0.1808-0.01960.8608-0.25570.1444-0.05710.0211-0.201-0.53220.30120.01610.1438-0.13420.15190.546-0.3376-0.01260.8965-0.25350.4161-36.3542-16.423-13.3387
350.2795-0.3552-0.11820.4942-0.08091.9236-0.28430.2573-0.2196-0.30390.19060.3210.1683-0.66430.23760.3407-0.20870.01320.9474-0.14860.444-47.0795-6.82914.1033
361.49020.2691-0.55891.2514-0.65212.2111-0.12530.4399-0.1982-0.4871-0.19340.04210.8032-0.42510.32320.5351-0.14160.04170.4542-0.16590.3323-24.6446-16.8357-18.4615
372.1157-0.1001-0.02410.89581.74824.15130.1548-0.3365-0.328-0.1163-0.2441-0.12360.9879-0.3586-0.00890.6184-0.21770.11190.4041-0.05720.4491-26.0557-22.7222.5616
381.93350.8236-0.85841.5801-0.1450.4232-0.0770.27520.0756-0.1943-0.00920.01050.4726-0.8845-0.00910.5523-0.1846-0.0250.7391-0.18420.313-33.8034-14.1294-15.6241
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 21 )A4 - 21
2X-RAY DIFFRACTION2chain 'A' and (resid 22 through 35 )A22 - 35
3X-RAY DIFFRACTION3chain 'A' and (resid 36 through 53 )A36 - 53
4X-RAY DIFFRACTION4chain 'A' and (resid 54 through 80 )A54 - 80
5X-RAY DIFFRACTION5chain 'A' and (resid 81 through 146 )A81 - 146
6X-RAY DIFFRACTION6chain 'A' and (resid 147 through 169 )A147 - 169
7X-RAY DIFFRACTION7chain 'A' and (resid 170 through 203 )A170 - 203
8X-RAY DIFFRACTION8chain 'A' and (resid 204 through 222 )A204 - 222
9X-RAY DIFFRACTION9chain 'A' and (resid 223 through 259 )A223 - 259
10X-RAY DIFFRACTION10chain 'B' and (resid 4 through 34 )B4 - 34
11X-RAY DIFFRACTION11chain 'B' and (resid 35 through 53 )B35 - 53
12X-RAY DIFFRACTION12chain 'B' and (resid 54 through 93 )B54 - 93
13X-RAY DIFFRACTION13chain 'B' and (resid 94 through 146 )B94 - 146
14X-RAY DIFFRACTION14chain 'B' and (resid 147 through 169 )B147 - 169
15X-RAY DIFFRACTION15chain 'B' and (resid 170 through 203 )B170 - 203
16X-RAY DIFFRACTION16chain 'B' and (resid 204 through 222 )B204 - 222
17X-RAY DIFFRACTION17chain 'B' and (resid 223 through 259 )B223 - 259
18X-RAY DIFFRACTION18chain 'C' and (resid 2 through 34 )C2 - 34
19X-RAY DIFFRACTION19chain 'C' and (resid 35 through 53 )C35 - 53
20X-RAY DIFFRACTION20chain 'C' and (resid 54 through 69 )C54 - 69
21X-RAY DIFFRACTION21chain 'C' and (resid 70 through 93 )C70 - 93
22X-RAY DIFFRACTION22chain 'C' and (resid 94 through 131 )C94 - 131
23X-RAY DIFFRACTION23chain 'C' and (resid 132 through 146 )C132 - 146
24X-RAY DIFFRACTION24chain 'C' and (resid 147 through 169 )C147 - 169
25X-RAY DIFFRACTION25chain 'C' and (resid 170 through 187 )C170 - 187
26X-RAY DIFFRACTION26chain 'C' and (resid 188 through 203 )C188 - 203
27X-RAY DIFFRACTION27chain 'C' and (resid 204 through 222 )C204 - 222
28X-RAY DIFFRACTION28chain 'C' and (resid 223 through 259 )C223 - 259
29X-RAY DIFFRACTION29chain 'D' and (resid 2 through 21 )D2 - 21
30X-RAY DIFFRACTION30chain 'D' and (resid 22 through 69 )D22 - 69
31X-RAY DIFFRACTION31chain 'D' and (resid 70 through 92 )D70 - 92
32X-RAY DIFFRACTION32chain 'D' and (resid 93 through 135 )D93 - 135
33X-RAY DIFFRACTION33chain 'D' and (resid 136 through 169 )D136 - 169
34X-RAY DIFFRACTION34chain 'D' and (resid 170 through 187 )D170 - 187
35X-RAY DIFFRACTION35chain 'D' and (resid 188 through 202 )D188 - 202
36X-RAY DIFFRACTION36chain 'D' and (resid 203 through 222 )D203 - 222
37X-RAY DIFFRACTION37chain 'D' and (resid 223 through 236 )D223 - 236
38X-RAY DIFFRACTION38chain 'D' and (resid 237 through 257 )D237 - 257

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