+Open data
-Basic information
Entry | Database: PDB / ID: 5ugy | ||||||||||||
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Title | Influenza hemagglutinin in complex with a neutralizing antibody | ||||||||||||
Components |
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Keywords | VIRAL PROTEIN/IMMUNE SYSTEM / influenza hemagglutinin / VIRAL PROTEIN-IMMUNE SYSTEM complex | ||||||||||||
Function / homology | Function and homology information immunoglobulin complex / immunoglobulin mediated immune response / viral budding from plasma membrane / antigen binding / clathrin-dependent endocytosis of virus by host cell / blood microparticle / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane ...immunoglobulin complex / immunoglobulin mediated immune response / viral budding from plasma membrane / antigen binding / clathrin-dependent endocytosis of virus by host cell / blood microparticle / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / extracellular region / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Influenza A virus Homo sapiens (human) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.801 Å | ||||||||||||
Authors | Whittle, J.R.R. / Jenni, S. / Harrison, S.C. | ||||||||||||
Citation | Journal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2011 Title: Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin. Authors: Whittle, J.R. / Zhang, R. / Khurana, S. / King, L.R. / Manischewitz, J. / Golding, H. / Dormitzer, P.R. / Haynes, B.F. / Walter, E.B. / Moody, M.A. / Kepler, T.B. / Liao, H.X. / Harrison, S.C. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5ugy.cif.gz | 993.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5ugy.ent.gz | 840.9 KB | Display | PDB format |
PDBx/mmJSON format | 5ugy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5ugy_validation.pdf.gz | 2.6 MB | Display | wwPDB validaton report |
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Full document | 5ugy_full_validation.pdf.gz | 2.6 MB | Display | |
Data in XML | 5ugy_validation.xml.gz | 90.1 KB | Display | |
Data in CIF | 5ugy_validation.cif.gz | 120.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ug/5ugy ftp://data.pdbj.org/pub/pdb/validation_reports/ug/5ugy | HTTPS FTP |
-Related structure data
Related structure data | 1rvzS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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-Components
-Hemagglutinin ... , 2 types, 6 molecules ACEBDF
#1: Protein | Mass: 36024.344 Da / Num. of mol.: 3 / Fragment: UNP residues 18-339 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/Solomon Islands/3/2006(H1N1)) Strain: A/Solomon Islands/3/2006(H1N1) / Gene: HA / Plasmid: pFastBac / Cell line (production host): HI-5 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: A7UPX0, UniProt: A7Y8A6*PLUS #2: Protein | Mass: 19841.041 Da / Num. of mol.: 3 / Fragment: UNP residues 344-516 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/Solomon Islands/3/2006(H1N1)) Strain: A/Solomon Islands/3/2006(H1N1) / Gene: HA / Plasmid: pFastBac / Cell line (production host): HI-5 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: A7UPX0, UniProt: A7Y8I1*PLUS |
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-Antibody , 2 types, 6 molecules HIJLMN
#3: Antibody | Mass: 24387.211 Da / Num. of mol.: 3 / Fragment: Fab Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293T / Production host: Homo sapiens (human) / References: UniProt: S6C4S0 #4: Antibody | Mass: 22272.625 Da / Num. of mol.: 3 / Fragment: Fab Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IGL@ / Cell line (production host): HEK293T / Production host: Homo sapiens (human) / References: UniProt: Q8N355 |
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-Sugars , 3 types, 15 molecules
#5: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #6: Polysaccharide | Source method: isolated from a genetically manipulated source #7: Sugar | ChemComp-NAG / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.05 Å3/Da / Density % sol: 69.64 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 2.2 M ammonium sulfate, 100 mM Tris, 5% PEG400, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.987 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 18, 2011 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Cryogenically-cooled single crystal Si(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.8→29.923 Å / Num. obs: 114569 / % possible obs: 94 % / Observed criterion σ(I): -3 / Redundancy: 5.702 % / Biso Wilson estimate: 40 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.145 / Rrim(I) all: 0.159 / Χ2: 0.997 / Net I/σ(I): 11.09 / Num. measured all: 653278 / Scaling rejects: 2338 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1RVZ Resolution: 2.801→29.923 Å / SU ML: 0.54 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 32.74
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 176.94 Å2 / Biso mean: 49.1023 Å2 / Biso min: 3.52 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.801→29.923 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 20
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