- PDB-5ubf: Crystal structure of the RctB domains 2-3, RctB-155-483 -
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基本情報
登録情報
データベース: PDB / ID: 5ubf
タイトル
Crystal structure of the RctB domains 2-3, RctB-155-483
要素
RctB replication initiator protein
キーワード
DNA BINDING PROTEIN / DNA replication initiation / DNA binding / secondary chromosome / Vibrio cholerae
機能・相同性
Replication initiator protein RctB, central region / RctB, helix turn helix domain / Vibrionales, replication initiator protein RctB, central region / RctB helix turn helix domain / DUF3346 domain-containing protein / Uncharacterized protein
温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6.5 詳細: Crystals of selenomethionine-substituted RctB domains 2-3 (155-483) were prepared by mixing 0.1, or 0.2, or 0.4 uL of the protein solution (22.7 mg/ml RctB-155-483 in 20 mM Tris pH 7.4, 500 ...詳細: Crystals of selenomethionine-substituted RctB domains 2-3 (155-483) were prepared by mixing 0.1, or 0.2, or 0.4 uL of the protein solution (22.7 mg/ml RctB-155-483 in 20 mM Tris pH 7.4, 500 mM sodium chloride, 5% glycerol, 5 mM 2-mercaptoethanol) and 0.2 uL of reservoir solution (0.1 M Bis-Tris propane pH 6.5, 0.2 M Magnesium chloride, 2% w/v PEG 8000).