- PDB-5ube: Crystal structure of the N-terminal domain (domain 1) of RctB -
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基本情報
登録情報
データベース: PDB / ID: 5ube
タイトル
Crystal structure of the N-terminal domain (domain 1) of RctB
要素
RctB replication initiator protein
キーワード
DNA BINDING PROTEIN / DNA replication initiation / DNA binding / secondary chromosome / Vibrio cholerae
機能・相同性
Replication initiator protein RctB, central region / RctB, helix turn helix domain / Vibrionales, replication initiator protein RctB, central region / RctB helix turn helix domain / DUF3346 domain-containing protein / Uncharacterized protein
温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7 詳細: Crystals of RctB domain 1 (1-124) were prepared using the sitting drop vapor diffusion method by mixing 0.1, or 0.2, or 0.4 uL of the protein solution (22.4 mg/ml RctB-2-124 in 20 mM Tris pH ...詳細: Crystals of RctB domain 1 (1-124) were prepared using the sitting drop vapor diffusion method by mixing 0.1, or 0.2, or 0.4 uL of the protein solution (22.4 mg/ml RctB-2-124 in 20 mM Tris pH 7.4, 50 mM sodium chloride, 5% glycerol, 5 mM 2-mercaptoethanol) and 0.2 uL of reservoir solution (0.24 M Sodium malonate pH 7.0, 20% w/v PEG 3350). Temp details: cold room