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- PDB-5tvf: Crystal structure of Trypanosoma brucei AdoMetDC/prozyme heterodi... -

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Basic information

Entry
Database: PDB / ID: 5tvf
TitleCrystal structure of Trypanosoma brucei AdoMetDC/prozyme heterodimer in complex with inhibitor CGP 40215
Components(S-adenosylmethionine decarboxylase ...) x 3
KeywordsLYASE / AdoMetDC / CGP40215 / decarboxylase / allostery / pseudoenzyme / prozyme
Function / homology
Function and homology information


spermine biosynthetic process / adenosylmethionine decarboxylase / adenosylmethionine decarboxylase activity / putrescine binding / spermidine biosynthetic process / lyase activity / cytosol
Similarity search - Function
: / S-adenosylmethionine decarboxylase / S-adenosylmethionine decarboxylase, conserved site / Adenosylmethionine decarboxylase / S-adenosylmethionine decarboxylase signature. / S-adenosylmethionine decarboxylase / S-adenosylmethionine decarboxylase / S-adenosylmethionine decarboxylase, core / 4-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Chem-CGQ / 1,4-DIAMINOBUTANE / S-adenosylmethionine decarboxylase proenzyme / S-adenosylmethionine decarboxylase proenzyme-like, putative
Similarity search - Component
Biological speciesTrypanosoma brucei brucei (eukaryote)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.42 Å
AuthorsPhillips, M.A. / Volkov, O.A. / Chen, Z. / Tomchick, D.R.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)2R37AI034432 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI090599 United States
CitationJournal: Elife / Year: 2016
Title: Relief of autoinhibition by conformational switch explains enzyme activation by a catalytically dead paralog.
Authors: Volkov, O.A. / Kinch, L. / Ariagno, C. / Deng, X. / Zhong, S. / Grishin, N. / Tomchick, D.R. / Chen, Z. / Phillips, M.A.
History
DepositionNov 8, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 11, 2017Provider: repository / Type: Initial release
Revision 1.1Sep 27, 2017Group: Advisory / Author supporting evidence
Category: pdbx_audit_support / pdbx_validate_polymer_linkage
Item: _pdbx_audit_support.funding_organization
Revision 1.2Dec 11, 2019Group: Advisory / Author supporting evidence
Category: pdbx_audit_support / pdbx_validate_polymer_linkage
Item: _pdbx_audit_support.funding_organization
Revision 1.3Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag
Revision 2.0Nov 15, 2023Group: Atomic model / Data collection / Derived calculations
Category: atom_site / chem_comp_atom ...atom_site / chem_comp_atom / chem_comp_bond / struct_conn
Item: _atom_site.auth_atom_id / _atom_site.label_atom_id ..._atom_site.auth_atom_id / _atom_site.label_atom_id / _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_1 / _chem_comp_bond.atom_id_2 / _struct_conn.ptnr1_label_atom_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: S-adenosylmethionine decarboxylase beta chain
B: S-adenosylmethionine decarboxylase alpha chain
C: S-adenosylmethionine decarboxylase beta chain
D: S-adenosylmethionine decarboxylase alpha chain
E: S-adenosylmethionine decarboxylase proenzyme-like, putative
F: S-adenosylmethionine decarboxylase proenzyme-like, putative
hetero molecules


Theoretical massNumber of molelcules
Total (without water)157,90214
Polymers156,2806
Non-polymers1,6228
Water4,684260
1
A: S-adenosylmethionine decarboxylase beta chain
B: S-adenosylmethionine decarboxylase alpha chain
F: S-adenosylmethionine decarboxylase proenzyme-like, putative
hetero molecules


Theoretical massNumber of molelcules
Total (without water)78,9517
Polymers78,1403
Non-polymers8114
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12820 Å2
ΔGint-50 kcal/mol
Surface area25500 Å2
MethodPISA
2
C: S-adenosylmethionine decarboxylase beta chain
D: S-adenosylmethionine decarboxylase alpha chain
E: S-adenosylmethionine decarboxylase proenzyme-like, putative
hetero molecules


Theoretical massNumber of molelcules
Total (without water)78,9517
Polymers78,1403
Non-polymers8114
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12850 Å2
ΔGint-46 kcal/mol
Surface area26190 Å2
MethodPISA
Unit cell
Length a, b, c (Å)81.126, 96.306, 98.483
Angle α, β, γ (deg.)90.00, 102.22, 90.00
Int Tables number4
Space group name H-MP1211

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Components

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S-adenosylmethionine decarboxylase ... , 3 types, 6 molecules ACBDEF

#1: Protein S-adenosylmethionine decarboxylase beta chain


Mass: 9754.132 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (strain 927/4 GUTat10.1) (eukaryote)
Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4410, Tb927.6.4460 / Plasmid: pETDuet-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21
References: UniProt: Q587A7, adenosylmethionine decarboxylase
#2: Protein S-adenosylmethionine decarboxylase alpha chain


Mass: 32041.992 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (strain 927/4 GUTat10.1) (eukaryote)
Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4410, Tb927.6.4460 / Plasmid: pETDuet-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21
References: UniProt: Q587A7, adenosylmethionine decarboxylase
#3: Protein S-adenosylmethionine decarboxylase proenzyme-like, putative


Mass: 36343.695 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (strain 927/4 GUTat10.1) (eukaryote)
Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4470 / Plasmid: pETDuet-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21
References: UniProt: Q587B3, adenosylmethionine decarboxylase

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Non-polymers , 4 types, 268 molecules

#4: Chemical ChemComp-B3P / 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL


Mass: 282.334 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C11H26N2O6 / Comment: pH buffer*YM
#5: Chemical ChemComp-CGQ / 3-[C-[N'-(3-CARBAMIMIDOYL-BENZYLIDENIUM)-HYDRAZINO]-[[AMINOMETHYLIDENE]AMINIUM]-IMINOMETHYL]-BENZAMIDINIUM / CGP40215A / BIS[[3-(AMINOIMINOMETHYL)PHENYL]METHYLENE] CARBONIMIDIC DIHYDRAZIDE


Mass: 352.417 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C17H22N9
#6: Chemical
ChemComp-PUT / 1,4-DIAMINOBUTANE / PUTRESCINE


Mass: 88.151 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C4H12N2
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 260 / Source method: isolated from a natural source / Formula: H2O

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Details

Sequence detailsPOSTTRANSLATIONAL MODIFICATION

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.4 Å3/Da / Density % sol: 48.9 % / Description: plate
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.9 / Details: 17 % PEG 3350, 0.1 M BTP, pH 6.9, 0.3 M NaCl

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97934 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 10, 2015
RadiationMonochromator: Graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97934 Å / Relative weight: 1
ReflectionResolution: 2.42→50 Å / Num. obs: 57137 / % possible obs: 99.4 % / Observed criterion σ(I): 1.7 / Redundancy: 4 % / Biso Wilson estimate: 25.1 Å2 / Rsym value: 0.109 / Net I/σ(I): 16.6
Reflection shellResolution: 2.42→2.46 Å / Redundancy: 3.4 % / Mean I/σ(I) obs: 1.7 / Num. unique all: 2789 / CC1/2: 0.53 / % possible all: 98.5

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Processing

Software
NameVersionClassification
PHENIX(1.10.1_2155: ???)refinement
HKL-3000data reduction
HKL-3000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5TVM

5tvm


Resolution: 2.42→32.376 Å / SU ML: 0.32 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 26.79 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2551 1674 3 %Random selection
Rwork0.217 ---
obs0.2181 55801 98.62 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.42→32.376 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10224 0 114 260 10598
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00210592
X-RAY DIFFRACTIONf_angle_d0.47614356
X-RAY DIFFRACTIONf_dihedral_angle_d12.1616227
X-RAY DIFFRACTIONf_chiral_restr0.0431553
X-RAY DIFFRACTIONf_plane_restr0.0031847
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.42-2.49120.34071280.29714119X-RAY DIFFRACTION91
2.4912-2.57160.32031370.2814467X-RAY DIFFRACTION98
2.5716-2.66340.36181400.2724501X-RAY DIFFRACTION99
2.6634-2.770.28361400.25084526X-RAY DIFFRACTION99
2.77-2.8960.27911400.24654537X-RAY DIFFRACTION99
2.896-3.04860.28751400.24434526X-RAY DIFFRACTION100
3.0486-3.23940.27511410.23414538X-RAY DIFFRACTION100
3.2394-3.48930.25921410.21974559X-RAY DIFFRACTION100
3.4893-3.83990.26131410.19844555X-RAY DIFFRACTION100
3.8399-4.39440.23961410.18644571X-RAY DIFFRACTION100
4.3944-5.5320.18831420.17224593X-RAY DIFFRACTION100
5.532-32.37870.211430.20074635X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.6371.4317-3.73775.8379-3.58043.348-0.09910.5028-0.2291-0.904-0.4134-0.44220.45280.19490.40150.38380.07130.06230.2738-0.01450.2089-27.640530.8356-13.2483
27.8276-1.29852.02455.39452.60242.19170.52150.53920.833-0.8257-0.40630.0042-0.66581.3465-0.11470.4350.06390.28190.41370.10190.4629-13.358530.9638-16.4981
33.0156-1.08650.4524.0782-1.04915.7636-0.212-0.2419-0.60930.21350.112-0.90940.68240.13430.10450.2046-0.0064-0.02820.30070.09150.5037-8.945117.315911.8347
45.59581.9337-0.10515.02081.79426.11120.0580.223-0.1091-0.31820.2195-1.47340.35590.7412-0.35960.09490.0628-0.04390.4430.07650.658-0.081421.96245.9586
52.43380.304-1.40132.1981-0.44620.9930.23380.09340.293-0.0840.0775-0.4591-0.30760.4339-0.21750.11660.00980.02990.31160.00820.41-9.50623.22885.9379
62.3619-0.9186-0.04673.6928-1.1133.6429-0.10260.0804-0.4391-0.024-0.2273-0.33290.2610.06120.28290.1447-0.05340.05170.196-0.0040.2685-17.480920.07642.3186
72.3333-0.98240.18578.6221-3.02042.81140.0295-0.0484-0.3253-0.27680.0957-0.00120.44610.0218-0.05540.1084-0.06370.01940.1846-0.01220.1618-27.528421.64142.7959
82.1404-0.43960.65871.97090.88341.16240.0227-0.04260.04410.0414-0.0615-0.3449-0.20030.28470.07570.1154-0.03720.03130.20010.02730.226-15.567934.05842.5528
93.73330.65761.32251.7802-0.58521.2824-0.12510.52170.5203-0.5909-0.2147-0.9949-0.25180.79420.13390.2105-0.08680.11710.54560.12440.92215.450334.0457-2.7205
101.7233-0.5850.02643.0329-0.16820.0054-0.02590.39780.69930.1357-0.0872-1.15010.12250.37060.28650.1079-0.09910.11820.44030.11610.4839-4.931934.0684-0.373
113.18220.1692-0.15851.2270.45832.3252-0.0814-0.23270.58940.20440.0285-0.4884-0.40370.234-0.00250.2245-0.0517-0.05480.1918-0.02990.4227-17.131244.02186.3092
127.352-0.64162.74253.1663-3.85617.5934-0.1404-0.22310.19550.5866-0.21920.0759-1.0436-0.15080.37510.3461-0.0433-0.03820.2508-0.04690.1491-24.30418.6437-31.4324
137.1194-2.4013-0.55144.81-2.89042.40590.3269-0.9829-0.44960.7363-0.5558-0.58921.0952-0.25690.14050.8216-0.2009-0.25690.4198-0.01440.2273-16.74853.2064-19.4699
144.2705-0.3576-1.1644.2816-0.83743.9278-0.1556-0.8125-0.00480.6936-0.21-0.28730.35021.02150.22540.2529-0.0215-0.12590.32530.07390.326-6.477911.2868-31.8573
151.32240.4188-1.50682.13151.16633.0463-0.0730.66840.2155-0.8372-0.0758-0.996-0.54420.0894-0.15060.5602-0.2340.14770.97170.43280.60455.921922.6914-58.6814
163.632-2.724-0.72122.7129-1.53077.5401-0.505-0.37060.52410.62710.1394-1.2377-1.13950.630.01640.4413-0.1344-0.1160.29930.18140.93756.432824.7998-41.2228
173.3827-0.30883.12525.34041.65095.957-0.1237-0.370.49530.25290.0277-0.7861-0.70640.41520.04110.2296-0.0393-0.15360.46620.13950.60319.359816.4746-37.5784
182.44080.62250.5042.7563-1.2774.8504-0.0531-0.2818-0.11680.2307-0.2963-0.53110.05931.04940.35240.2479-0.0196-0.08320.31780.13950.38240.719615.3953-41.2151
192.6580.66980.55463.5671-0.83961.6972-0.15120.1960.3939-0.0647-0.0736-0.1946-0.34330.08530.16820.33140.024-0.0550.23420.05920.229-11.128518.0959-42.48
202.4638-0.28560.48181.920.91962.3403-0.08510.41490.2605-0.5876-0.1374-0.42440.19210.62330.20830.44080.02790.0240.33360.09180.2346-10.55429.7186-50.0377
212.96210.37841.09881.7556-0.37122.27650.1606-0.1792-0.4140.3256-0.176-0.60040.13090.47210.0870.30740.0491-0.11060.37030.18760.46885.60922.8888-30.214
223.4114-0.60790.97913.254-0.58442.18420.1380.5212-0.6183-0.4843-0.225-0.70780.73190.49640.05190.35370.12030.03420.3624-0.01040.5088-2.8481-5.9646-45.3441
233.21930.4572-0.58610.99261.20791.9044-0.0620.168-0.49550.02090.0372-0.49150.3264-0.1563-0.09840.23280.0308-0.04460.21890.04090.2535-12.7197-3.8924-44.7016
242.7462.39372.24112.11691.93561.77270.0742-0.36630.66370.479-0.36980.4005-0.4431-0.18990.27450.5929-0.04910.04990.4176-0.08040.388-28.543714.6842-26.684
252.2781-0.1008-0.10193.5998-0.52281.99390.0851-0.1293-0.0564-0.4734-0.13760.29210.2211-0.3243-0.03330.2522-0.0074-0.07690.2158-0.07820.218-41.5961-10.2215-43.1361
264.24330.99521.76235.4568-0.77163.8820.0809-0.0405-0.2287-0.23150.37790.7204-0.1942-0.6558-0.26270.2298-0.0578-0.01030.4376-0.05110.4949-52.9966-4.1679-43.5103
272.96411.2644-0.01295.172-0.74081.75620.08010.10.1570.1237-0.22590.67720.0049-0.15180.14820.25030.0206-0.01230.2235-0.07360.2574-45.0822-4.9335-43.1697
283.22461.6994-0.65892.6038-0.40062.34630.03510.120.4020.15820.02060.2007-0.2905-0.0677-0.05750.27390.0161-0.02580.1709-0.03410.1613-31.45533.1115-40.8158
293.99410.9553-0.01112.5171-0.55145.32640.35450.0206-0.9054-0.5972-0.06470.23211.18210.1457-0.30160.3638-0.0245-0.07130.2848-0.02780.3056-30.4046-13.2386-47.1373
306.82760.7354-0.18978.3768-15.3926-0.1680.36420.2793-0.01760.07620.14440.1822-0.27290.08260.3852-0.0031-0.09550.3366-0.00990.1802-40.8821-3.5558-58.705
312.2668-0.86960.94663.2942-0.58713.0715-0.35980.64790.0138-1.27880.0403-0.550.75920.0840.13880.728-0.04640.01690.4259-0.03420.1766-29.5283-2.4807-62.29
322.3906-0.20360.78393.0611-2.00164.4862-0.1170.66710.0662-0.51720.1950.1349-0.240.1849-0.02140.37880.0108-0.00280.37960.02040.1475-23.0645-0.0307-55.6688
332.8751-3.1-0.3683.96311.36361.27620.00530.2303-0.333-0.7851-0.31760.23210.13910.45260.35910.5950.06930.02920.4502-0.00850.3471-30.368824.1665-19.0963
343.05080.06060.49653.8067-0.90081.60250.13610.19980.1255-0.54390.00130.43480.1903-0.2191-0.09360.1675-0.036-0.02890.2583-0.01110.1704-54.159145.8961-11.4591
352.797-0.68230.2865.3691-1.50612.6950.05210.0404-0.0539-0.366-0.00890.41790.2138-0.1266-0.03420.1784-0.0255-0.02980.1713-0.01650.1642-52.033843.7162-10.6558
362.3646-1.48260.54442.6888-0.44841.66550.1269-0.0445-0.3314-0.3723-0.05960.27210.3165-0.0693-0.04750.1883-0.0141-0.00390.1387-0.01270.1499-38.657335.6148-7.397
372.42540.1034-1.26111.1411-0.44423.68970.3036-0.03090.74910.5641-0.02120.3213-0.8597-0.1631-0.23670.23770.02950.04760.15420.02090.3883-40.166252.186-1.1891
383.9167-0.47750.49342.9126-2.28557.4362-0.243-0.524-0.33970.59570.16310.4242-0.2987-0.440.05180.27680.02920.1260.32190.01850.2545-53.708942.43057.8788
392.1223-0.4509-1.54921.3662-2.1986.10010.0809-0.34780.20361.16110.11140.1892-0.73360.0046-0.04770.38950.03510.02970.2849-0.010.1729-41.918441.455611.6221
405.22052.5049-2.00935.2998-2.17214.5959-0.0355-0.60750.07280.42730.16660.12240.0260.0977-0.07590.20250.01590.01610.1862-0.00370.1107-36.580639.43739.1677
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 5 through 19 )
2X-RAY DIFFRACTION2chain 'A' and (resid 20 through 30 )
3X-RAY DIFFRACTION3chain 'A' and (resid 31 through 65 )
4X-RAY DIFFRACTION4chain 'A' and (resid 66 through 85 )
5X-RAY DIFFRACTION5chain 'B' and (resid 87 through 104 )
6X-RAY DIFFRACTION6chain 'B' and (resid 105 through 145 )
7X-RAY DIFFRACTION7chain 'B' and (resid 146 through 166 )
8X-RAY DIFFRACTION8chain 'B' and (resid 167 through 207 )
9X-RAY DIFFRACTION9chain 'B' and (resid 208 through 247 )
10X-RAY DIFFRACTION10chain 'B' and (resid 248 through 276 )
11X-RAY DIFFRACTION11chain 'B' and (resid 277 through 356 )
12X-RAY DIFFRACTION12chain 'C' and (resid 5 through 18 )
13X-RAY DIFFRACTION13chain 'C' and (resid 19 through 23 )
14X-RAY DIFFRACTION14chain 'C' and (resid 24 through 38 )
15X-RAY DIFFRACTION15chain 'C' and (resid 39 through 48 )
16X-RAY DIFFRACTION16chain 'C' and (resid 49 through 65 )
17X-RAY DIFFRACTION17chain 'C' and (resid 66 through 85 )
18X-RAY DIFFRACTION18chain 'D' and (resid 87 through 104 )
19X-RAY DIFFRACTION19chain 'D' and (resid 105 through 166 )
20X-RAY DIFFRACTION20chain 'D' and (resid 167 through 189 )
21X-RAY DIFFRACTION21chain 'D' and (resid 190 through 276 )
22X-RAY DIFFRACTION22chain 'D' and (resid 277 through 330 )
23X-RAY DIFFRACTION23chain 'D' and (resid 331 through 356 )
24X-RAY DIFFRACTION24chain 'E' and (resid 4 through 35 )
25X-RAY DIFFRACTION25chain 'E' and (resid 36 through 55 )
26X-RAY DIFFRACTION26chain 'E' and (resid 56 through 76 )
27X-RAY DIFFRACTION27chain 'E' and (resid 77 through 119 )
28X-RAY DIFFRACTION28chain 'E' and (resid 120 through 181 )
29X-RAY DIFFRACTION29chain 'E' and (resid 182 through 203 )
30X-RAY DIFFRACTION30chain 'E' and (resid 204 through 258 )
31X-RAY DIFFRACTION31chain 'E' and (resid 259 through 299 )
32X-RAY DIFFRACTION32chain 'E' and (resid 300 through 325 )
33X-RAY DIFFRACTION33chain 'F' and (resid 4 through 35 )
34X-RAY DIFFRACTION34chain 'F' and (resid 36 through 76 )
35X-RAY DIFFRACTION35chain 'F' and (resid 77 through 119 )
36X-RAY DIFFRACTION36chain 'F' and (resid 120 through 181 )
37X-RAY DIFFRACTION37chain 'F' and (resid 182 through 203 )
38X-RAY DIFFRACTION38chain 'F' and (resid 204 through 270 )
39X-RAY DIFFRACTION39chain 'F' and (resid 271 through 299 )
40X-RAY DIFFRACTION40chain 'F' and (resid 300 through 324 )

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