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- PDB-5tno: Discovery of novel aminobenzisoxazole derivatives as orally avail... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5tno | ||||||
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Title | Discovery of novel aminobenzisoxazole derivatives as orally available factor IXa inhibitors | ||||||
![]() | (Coagulation factor ...) x 2 | ||||||
![]() | HYDROLASE/HYDROLASE inhibitor / SERINE PROTEINASE / BLOOD COAGULATION / COAGULATION FACTOR / HYDROLASE-2 / HYDROLASE INHIBITOR COMPLEX / HYDROLASE-HYDROLASE INHIBITOR COMPLEX | ||||||
Function / homology | ![]() Defective F9 secretion / Defective gamma-carboxylation of F9 / coagulation factor IXa / Defective F9 activation / Defective factor IX causes thrombophilia / Defective cofactor function of FVIIIa variant / Defective F9 variant does not activate FX / zymogen activation / Extrinsic Pathway of Fibrin Clot Formation / Protein hydroxylation ...Defective F9 secretion / Defective gamma-carboxylation of F9 / coagulation factor IXa / Defective F9 activation / Defective factor IX causes thrombophilia / Defective cofactor function of FVIIIa variant / Defective F9 variant does not activate FX / zymogen activation / Extrinsic Pathway of Fibrin Clot Formation / Protein hydroxylation / Gamma-carboxylation of protein precursors / Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus / Removal of aminoterminal propeptides from gamma-carboxylated proteins / Intrinsic Pathway of Fibrin Clot Formation / Golgi lumen / blood coagulation / collagen-containing extracellular matrix / endopeptidase activity / endoplasmic reticulum lumen / serine-type endopeptidase activity / calcium ion binding / proteolysis / extracellular space / extracellular exosome / extracellular region / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sakurada, I. / Endo, T. / Hikita, K. / Hirabayashi, T. / Hosaka, Y. / Kato, Y. / Maeda, Y. / Matsumoto, S. / Mizuno, T. / Nagasue, H. ...Sakurada, I. / Endo, T. / Hikita, K. / Hirabayashi, T. / Hosaka, Y. / Kato, Y. / Maeda, Y. / Matsumoto, S. / Mizuno, T. / Nagasue, H. / Nishimura, T. / Shimada, S. / Shinozaki, M. / Taguchi, K. / Takeuchi, K. / Yokoyama, T. / Hruza, A. / Reichert, P. / Zhang, T. / Wood, H.B. / Nakao, K. / Furusako, S. | ||||||
![]() | ![]() Title: Discovery of novel aminobenzisoxazole derivatives as orally available factor IXa inhibitors. Authors: Sakurada, I. / Endo, T. / Hikita, K. / Hirabayashi, T. / Hosaka, Y. / Kato, Y. / Maeda, Y. / Matsumoto, S. / Mizuno, T. / Nagasue, H. / Nishimura, T. / Shimada, S. / Shinozaki, M. / Taguchi, ...Authors: Sakurada, I. / Endo, T. / Hikita, K. / Hirabayashi, T. / Hosaka, Y. / Kato, Y. / Maeda, Y. / Matsumoto, S. / Mizuno, T. / Nagasue, H. / Nishimura, T. / Shimada, S. / Shinozaki, M. / Taguchi, K. / Takeuchi, K. / Yokoyama, T. / Hruza, A. / Reichert, P. / Zhang, T. / Wood, H.B. / Nakao, K. / Furusako, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 248.3 KB | Display | ![]() |
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PDB format | ![]() | 199.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5tntC ![]() 1rfnS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Coagulation factor ... , 2 types, 2 molecules AB
#1: Protein | Mass: 26104.703 Da / Num. of mol.: 1 / Fragment: UNP residues 227-461 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein | Mass: 6841.809 Da / Num. of mol.: 1 / Fragment: UNP residues 131-191 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Non-polymers , 4 types, 258 molecules ![](data/chem/img/NA.gif)
![](data/chem/img/NHE.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NHE.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-UNL / Num. of mol.: 1 / Source method: obtained synthetically | ||
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#4: Chemical | ChemComp-NA / | ||
#5: Chemical | #6: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.56 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 9 Details: 50 MM CHES, PH 9.0, 1.3 M TRI-SODIUM CITRATE AND 3 MM COMPOUND (CROSS SEEDED WITH CRYSTALS GROWN FROM REMARK 280 50 MM TRIS, PH 7.2, 1.45 M AMMONIUM SULFATE, 2.0 M SODIUM REMARK 280 CHLORIDE AND 3 MM COMPOUND) |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 14, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.537→63.36 Å / Num. obs: 51117 / % possible obs: 99.9 % / Redundancy: 5.1 % / Biso Wilson estimate: 25.14 Å2 / Rmerge(I) obs: 0.03 / Net I/σ(I): 25.7 |
Reflection shell | Resolution: 1.537→1.542 Å / Redundancy: 5.2 % / Rmerge(I) obs: 0.524 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1RFN Resolution: 1.54→22.55 Å / Cor.coef. Fo:Fc: 0.973 / Cor.coef. Fo:Fc free: 0.97 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.063 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.057 / SU Rfree Blow DPI: 0.055 / SU Rfree Cruickshank DPI: 0.055
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Displacement parameters | Biso mean: 32.9 Å2
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Refine analyze | Luzzati coordinate error obs: 0.15 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.54→22.55 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.54→1.58 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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