Mass: 53591.992 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: 34 N-terminal residues are replaced with the exogenous sequence MAKKTSSKGKL 6xHis tag is introduced at the C-terminus CYS 371 and CYS 392 are spontaneously oxidized to sulfenic acid Source: (gene. exp.) Naegleria fowleri (brain-eating amoeba) Gene: NF0102700 / Plasmid: pCW-LIC / Production host: Escherichia coli (E. coli) / Strain (production host): DH5alpha / References: UniProt: A0A2H4A2U9*PLUS
Resolution: 1.71→7.63 Å / Cor.coef. Fo:Fc: 0.973 / Cor.coef. Fo:Fc free: 0.952 / SU B: 3.712 / SU ML: 0.116 / Cross valid method: THROUGHOUT / ESU R: 0.119 / ESU R Free: 0.125 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2486
2522
5.1 %
RANDOM
Rwork
0.19484
-
-
-
obs
0.1976
47085
98.55 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å