- PDB-5tf2: CRYSTAL STRUCTURE OF THE WD40 DOMAIN OF THE HUMAN PROLACTIN REGUL... -
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Basic information
Entry
Database: PDB / ID: 5tf2
Title
CRYSTAL STRUCTURE OF THE WD40 DOMAIN OF THE HUMAN PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN
Components
Prolactin regulatory element-binding protein
Keywords
TRANSCRIPTION / WD40 / Activator / Protein Transport / Structural Genomics Consortium (SGC)
Function / homology
Function and homology information
regulation of COPII vesicle coating / : / protein exit from endoplasmic reticulum / COPII vesicle coating / XBP1(S) activates chaperone genes / Cargo concentration in the ER / COPII-mediated vesicle transport / endoplasmic reticulum exit site / protein secretion / endoplasmic reticulum to Golgi vesicle-mediated transport ...regulation of COPII vesicle coating / : / protein exit from endoplasmic reticulum / COPII vesicle coating / XBP1(S) activates chaperone genes / Cargo concentration in the ER / COPII-mediated vesicle transport / endoplasmic reticulum exit site / protein secretion / endoplasmic reticulum to Golgi vesicle-mediated transport / GTPase activator activity / guanyl-nucleotide exchange factor activity / GTPase binding / endoplasmic reticulum membrane / DNA binding / membrane / nucleus Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 20 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.4 Å3/Da / Density % sol: 63.81 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: PREB protein was mixed with elastase at 1:1000 (w/w) ratio before setting up crystallization. Crystals were grown at 293 K using the sitting drop method by mixing 0.5 uL protein with 0.5 uL ...Details: PREB protein was mixed with elastase at 1:1000 (w/w) ratio before setting up crystallization. Crystals were grown at 293 K using the sitting drop method by mixing 0.5 uL protein with 0.5 uL well solution consisting of 1.4M Malonate, pH 7.0. The crystals were cryoprotected by immersion in Paratone.
Resolution: 2.8→50 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.926 / SU B: 21.049 / SU ML: 0.183 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.384 / ESU R Free: 0.25 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.214
784
4.9 %
RANDOM
Rwork
0.1888
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obs
0.19
15265
99.93 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å
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