A: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30 B: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30 C: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30 D: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30 E: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30 F: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30 G: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30 H: Fusion protein of Nucleoprotein and Minor nucleoprotein VP30
FusionproteinofNucleoproteinandMinornucleoproteinVP30 / Nucleocapsid protein / Protein N / Transcription activator VP30
Mass: 20169.670 Da / Num. of mol.: 8 Fragment: UNP P27588 residues 552-579,UNP P35258 residues 146-281 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lake Victoria marburgvirus (strain Musoke-80) Strain: Musoke-80 / Gene: NP, VP30 / Plasmid: pET46 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta2 pLysS / References: UniProt: P27588, UniProt: P35258
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.79 Å3/Da / Density % sol: 67.5 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 20% isopropanol, 18% PEG 4000, 0.1M sodium citrate pH 5.6 using 0.2 uL 24.7 mg/mL protein mixed with 0.2 uL mother liquor