+
データを開く
-
基本情報
登録情報 | データベース: PDB / ID: 5t04 | ||||||
---|---|---|---|---|---|---|---|
タイトル | STRUCTURE OF CONSTITUTIVELY ACTIVE NEUROTENSIN RECEPTOR | ||||||
![]() |
| ||||||
![]() | SIGNALING PROTEIN / MEMBRANE PROTEIN / G PROTEIN-COUPLED RECEPTOR / GPCR / NEUROTENSIN RECEPTOR / NTSR1 / agonist | ||||||
機能・相同性 | ![]() regulation of locomotion involved in locomotory behavior / Peptide ligand-binding receptors / positive regulation of locomotion / neuropeptide receptor binding / G protein-coupled neurotensin receptor activity / regulation of inositol trisphosphate biosynthetic process / inositol phosphate catabolic process / symmetric synapse / positive regulation of gamma-aminobutyric acid secretion / D-aspartate import across plasma membrane ...regulation of locomotion involved in locomotory behavior / Peptide ligand-binding receptors / positive regulation of locomotion / neuropeptide receptor binding / G protein-coupled neurotensin receptor activity / regulation of inositol trisphosphate biosynthetic process / inositol phosphate catabolic process / symmetric synapse / positive regulation of gamma-aminobutyric acid secretion / D-aspartate import across plasma membrane / regulation of membrane depolarization / positive regulation of arachidonate secretion / vocalization behavior / response to antipsychotic drug / neuron spine / L-glutamate import across plasma membrane / regulation of behavioral fear response / regulation of respiratory gaseous exchange / neuropeptide hormone activity / cAMP biosynthetic process / positive regulation of inhibitory postsynaptic potential / negative regulation of systemic arterial blood pressure / negative regulation of release of sequestered calcium ion into cytosol / digestive tract development / positive regulation of glutamate secretion / hyperosmotic response / G alpha (q) signalling events / response to mineralocorticoid / response to food / positive regulation of inositol phosphate biosynthetic process / response to lipid / response to corticosterone / cellular response to lithium ion / temperature homeostasis / response to stress / detection of temperature stimulus involved in sensory perception of pain / associative learning / conditioned place preference / neuropeptide signaling pathway / cellular response to dexamethasone stimulus / response to axon injury / viral release from host cell by cytolysis / transport vesicle / axon terminus / peptidoglycan catabolic process / positive regulation of release of sequestered calcium ion into cytosol / response to amphetamine / blood vessel diameter maintenance / adult locomotory behavior / dendritic shaft / learning / response to cocaine / liver development / visual learning / cellular response to nerve growth factor stimulus / cytoplasmic side of plasma membrane / terminal bouton / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / response to estradiol / perikaryon / dendritic spine / host cell cytoplasm / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / defense response to bacterium / positive regulation of apoptotic process / membrane raft / receptor ligand activity / axon / negative regulation of gene expression / neuronal cell body / dendrite / positive regulation of gene expression / negative regulation of apoptotic process / protein-containing complex binding / cell surface / extracellular region / identical protein binding / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Krumm, B. / Botos, I. / Grisshammer, R. | ||||||
![]() | ![]() タイトル: Structure and dynamics of a constitutively active neurotensin receptor. 著者: Krumm, B.E. / Lee, S. / Bhattacharya, S. / Botos, I. / White, C.F. / Du, H. / Vaidehi, N. / Grisshammer, R. | ||||||
履歴 |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-
ダウンロード
PDBx/mmCIF形式 | ![]() | 201.2 KB | 表示 | ![]() |
---|---|---|---|---|
PDB形式 | ![]() | 161.3 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 717.4 KB | 表示 | ![]() |
---|---|---|---|---|
文書・詳細版 | ![]() | 720.9 KB | 表示 | |
XML形式データ | ![]() | 18.2 KB | 表示 | |
CIF形式データ | ![]() | 23.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 4xeeS S: 精密化の開始モデル |
---|---|
類似構造データ |
-
リンク
-
集合体
登録構造単位 | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
単位格子 |
|
-
要素
#1: タンパク質 | 分子量: 57613.445 Da / 分子数: 1 断片: unp residues 43-268; 2-161; 297-396,unp residues 43-268; 2-161; 297-396,unp residues 43-268; 2-161; 297-396 変異: A86L, G215A, F358A, V360A, R12G, C54T, C97A, Q122N, Q123N, I137R,A86L, G215A, F358A, V360A, R12G, C54T, C97A, Q122N, Q123N, I137R,A86L, G215A, F358A, V360A, R12G, C54T, C97A, Q122N, Q123N, I137R 由来タイプ: 組換発現 由来: (組換発現) ![]() ![]() ![]() 遺伝子: Ntsr1, Ntsr / プラスミド: PFASTBAC1 / 発現宿主: ![]() | ||
---|---|---|---|
#2: タンパク質・ペプチド | 分子量: 819.007 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) ![]() ![]() | ||
#3: 化合物 | ChemComp-TCE / | ||
#4: 化合物 | ChemComp-GOL / | ||
#5: 化合物 | Has protein modification | Y | |
-実験情報
-実験
実験 | 手法: ![]() |
---|
-
試料調製
結晶 | マシュー密度: 2.81 Å3/Da / 溶媒含有率: 56.2 % |
---|---|
結晶化 | 温度: 293 K / 手法: 脂質キュービック相法 詳細: 13-16% (v/v) PEG 400, 80 mM TrisHCl pH 8.5-9.0, 1.9 mM TCEP, 68-91 mM lithium acetate, 0.9 mM Neurotensin PH範囲: 8.5-9.0 |
-データ収集
回折 |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
放射光源 |
| |||||||||||||||
検出器 |
| |||||||||||||||
放射 |
| |||||||||||||||
放射波長 |
| |||||||||||||||
反射 | 解像度: 3.3→38.141 Å / Num. obs: 10042 / % possible obs: 97.3 % / 冗長度: 7.1 % / Rmerge(I) obs: 0.18 / Net I/σ(I): 8.3 | |||||||||||||||
反射 シェル | 解像度: 3.3→3.56 Å / 冗長度: 3.7 % / Rmerge(I) obs: 0.72 / Mean I/σ(I) obs: 1.6 / % possible all: 88.3 |
-
解析
ソフトウェア |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 構造決定の手法: ![]() 開始モデル: 4XEE 解像度: 3.3→38.141 Å / SU ML: 0.47 / 交差検証法: FREE R-VALUE / σ(F): 0.01 / 位相誤差: 30.97 / 立体化学のターゲット値: ML
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 3.3→38.141 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS精密化 シェル |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 TLS | 手法: refined / Origin x: 228.6574 Å / Origin y: 16.6097 Å / Origin z: 96.5727 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 TLSグループ | Selection details: all |