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Open data
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Basic information
Entry | Database: PDB / ID: 5prn | ||||||
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Title | E1M, Y96W, S119W MUTANT OF RH. BLASTICA PORIN | ||||||
![]() | PORIN![]() | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Maveyraud, L. / Schmid, B. / Schulz, G.E. | ||||||
![]() | ![]() Title: Porin mutants with new channel properties. Authors: Schmid, B. / Maveyraud, L. / Kromer, M. / Schulz, G.E. #1: ![]() Title: Expression of Porin from Rhodopseudomonas Blastica in Escherichia Coli Inclusion Bodies and Folding Into Exact Native Structure Authors: Schmid, B. / Kromer, M. / Schulz, G.E. #2: ![]() Title: Refined Structure of the Porin from Rhodopseudomonas Blastica. Comparison with the Porin from Rhodobacter Capsulatus Authors: Kreusch, A. / Schulz, G.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.5 KB | Display | ![]() |
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PDB format | ![]() | 53.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1bh3C ![]() 2prnC ![]() 3prnC ![]() 6prnC ![]() 7prnC ![]() 8prnC ![]() 1prnS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 30733.104 Da / Num. of mol.: 1 / Mutation: E1M, Y96W, S119W Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.27 Å3/Da / Density % sol: 66 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 7.8 / Details: pH 7.8 | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.2 / Method: vapor diffusion, hanging drop / Details: Kreusch, A., (1994) J.Mol.Biol., 243, 891. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Oct 1, 1996 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→12 Å / Num. obs: 31979 / % possible obs: 92 % / Observed criterion σ(I): 4 / Redundancy: 2.2 % / Biso Wilson estimate: 21.7 Å2 / Rmerge(I) obs: 0.046 / Rsym value: 0.046 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 1.4 % / Rmerge(I) obs: 0.142 / Mean I/σ(I) obs: 3.4 / Rsym value: 0.142 / % possible all: 78.1 |
Reflection shell | *PLUS % possible obs: 78 % / Num. unique obs: 2657 |
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Processing
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Refinement | Method to determine structure![]() Starting model: PDB ENTRY 1PRN Resolution: 2→12 Å / Cross valid method: THROUGHOUT / σ(F): 1
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Displacement parameters | Biso mean: 27.89 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→12 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.163 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |