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Open data
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Basic information
Entry | Database: PDB / ID: 1h6s | ||||||
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Title | Asymmetric conductivity of engineered proteins | ||||||
![]() | PORIN | ||||||
![]() | MEMBRANE PROTEIN / INTEGRAL MEMBRANE PROTEIN PORIN | ||||||
Function / homology | ![]() porin activity / pore complex / monoatomic ion transport / cell outer membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Bannwarth, M. / Schulz, G.E. | ||||||
![]() | ![]() Title: Asymmetric Conductivity of Engineered Porins Authors: Bannwarth, M. / Schulz, G.E. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "1A" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "1A" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 16-STRANDED BARREL THIS IS REPRESENTED BY A 17-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 65.1 KB | Display | ![]() |
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PDB format | ![]() | 48 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 411 KB | Display | ![]() |
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Full document | ![]() | 414.5 KB | Display | |
Data in XML | ![]() | 11.9 KB | Display | |
Data in CIF | ![]() | 15 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1prnS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32222.725 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Details: INSERT SEQUENCE, GGGGPKLAKMEKARGGGG / Source: (gene. exp.) ![]() Description: RECOMBINANT ESCHERICHIA COLI. SYNTHETIC DNA INSERTION Plasmid: PET-3B-INS41L / Production host: ![]() ![]() |
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Compound details | CHAIN 1 AN 18 AMINO ACID INSERTION BETWEEN ASN 203 AND ASP 203 CHAIN 1 ENGINEERED MUTAION E1M: ...CHAIN 1 AN 18 AMINO ACID INSERTION BETWEEN ASN 203 AND ASP 203 CHAIN 1 ENGINEERED |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.05 Å3/Da / Density % sol: 69.63 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.8 Details: 50 MM NAOAC PH 4.75, 100 MM (NH4)2SO4, 5.5 % PEG4000 | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 4.75 / Method: unknown | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: IMAGE PLATE / Date: Oct 14, 2000 |
Radiation | Monochromator: NI-FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3→36 Å / Num. obs: 10136 / % possible obs: 99 % / Observed criterion σ(I): 3 / Redundancy: 6.6 % / Biso Wilson estimate: 37.904 Å2 / Net I/σ(I): 19.1 |
Reflection shell | Resolution: 3→3.03 Å / Redundancy: 6.5 % / Mean I/σ(I) obs: 5.1 / % possible all: 99 |
Reflection | *PLUS % possible obs: 99 % / Num. measured all: 66659 / Rmerge(I) obs: 0.081 |
Reflection shell | *PLUS Highest resolution: 3 Å / Lowest resolution: 3.15 Å / Num. unique obs: 1498 / Num. measured obs: 9696 / Rmerge(I) obs: 0.184 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1PRN Resolution: 3→36 Å / Data cutoff high absF: 10000 / Cross valid method: RMSD / σ(F): 3 Details: INSERT REGION IS DISORDERED ONLY MISSING BOND IN COMPARISON TO WT IS SEEN.
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Displacement parameters | Biso mean: 22.268 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2882 Å / Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 0.2253 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→36 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.14 Å / Total num. of bins used: 8
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Refinement | *PLUS Rfactor Rfree: 0.297 / Rfactor Rwork: 0.21 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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