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Open data
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Basic information
Entry | Database: PDB / ID: 3prn | ||||||
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Title | E1M, A104W MUTANT OF RH. BLASTICA PORIN | ||||||
![]() | PORIN | ||||||
![]() | MEMBRANE PROTEIN / INTEGRAL MEMBRANE PROTEIN / PORIN / PORE EYELET MUTANT | ||||||
Function / homology | ![]() porin activity / pore complex / monoatomic ion transport / cell outer membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Maveyraud, L. / Schmid, B. / Schulz, G.E. | ||||||
![]() | ![]() Title: Porin mutants with new channel properties. Authors: Schmid, B. / Maveyraud, L. / Kromer, M. / Schulz, G.E. #1: ![]() Title: Expression of Porin from Rhodopseudomonas Blastica in Escherichia Coli Inclusion Bodies and Folding Into Exact Native Structure Authors: Schmid, B. / Kromer, M. / Schulz, G.E. #2: ![]() Title: Refined Structure of the Porin from Rhodopseudomonas Blastica. Comparison with the Porin from Rhodobacter Capsulatus Authors: Kreusch, A. / Schulz, G.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.4 KB | Display | ![]() |
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PDB format | ![]() | 53 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 872.8 KB | Display | ![]() |
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Full document | ![]() | 900 KB | Display | |
Data in XML | ![]() | 15.1 KB | Display | |
Data in CIF | ![]() | 20.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1bh3C ![]() 2prnC ![]() 5prnC ![]() 6prnC ![]() 7prnC ![]() 8prnC ![]() 1prnS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30726.068 Da / Num. of mol.: 1 / Mutation: E1M, A104W Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-MG / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.28 Å3/Da / Density % sol: 66 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.8 / Details: pH 7.8 | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.2 / Method: vapor diffusion, hanging drop / Details: Kreusch, A., (1994) J.Mol.Biol., 243, 891. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Dec 1, 1996 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→12.8 Å / Num. obs: 34853 / % possible obs: 85.7 % / Redundancy: 2.5 % / Biso Wilson estimate: 20.9 Å2 / Rmerge(I) obs: 0.038 / Rsym value: 0.038 / Net I/σ(I): 15.9 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 1.3 % / Rmerge(I) obs: 0.19 / Mean I/σ(I) obs: 2.5 / Rsym value: 0.19 / % possible all: 51 |
Reflection shell | *PLUS % possible obs: 51 % / Num. unique obs: 2094 |
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Processing
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Refinement | Method to determine structure: DIFFERENCE FOURIER Starting model: PDB ENTRY 1PRN Resolution: 1.9→12.8 Å / Cross valid method: THROUGHOUT / σ(F): 1
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Displacement parameters | Biso mean: 31.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→12.8 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.169 / Rfactor Rfree: 0.19 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |