IRON/SULFUR CLUSTER / DNA / DNA (> 10) / DNA (> 100) / DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 ...IRON/SULFUR CLUSTER / DNA / DNA (> 10) / DNA (> 100) / DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Transcription initiation factor IIA large subunit / Transcription initiation factor IIA subunit 2 / General transcription and DNA repair factor IIH subunit TFB1 / DNA-directed RNA polymerase II subunit RPB7 / Transcription initiation factor IIE subunit alpha / Transcription initiation factor IIE subunit beta / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / Transcription initiation factor IIF subunit alpha / Transcription initiation factor IIF subunit beta / General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 / General transcription and DNA repair factor IIH subunit TFB2 / RNA polymerase II transcription factor B subunit 3 / General transcription and DNA repair factor IIH subunit SSL1 / General transcription and DNA repair factor IIH subunit TFB4 / General transcription and DNA repair factor IIH subunit TFB5 類似検索 - 構成要素
ジャーナル: Nature / 年: 2017 タイトル: Structures of transcription pre-initiation complex with TFIIH and Mediator. 著者: S Schilbach / M Hantsche / D Tegunov / C Dienemann / C Wigge / H Urlaub / P Cramer / 要旨: For the initiation of transcription, RNA polymerase II (Pol II) assembles with general transcription factors on promoter DNA to form the pre-initiation complex (PIC). Here we report cryo-electron ...For the initiation of transcription, RNA polymerase II (Pol II) assembles with general transcription factors on promoter DNA to form the pre-initiation complex (PIC). Here we report cryo-electron microscopy structures of the Saccharomyces cerevisiae PIC and PIC-core Mediator complex at nominal resolutions of 4.7 Å and 5.8 Å, respectively. The structures reveal transcription factor IIH (TFIIH), and suggest how the core and kinase TFIIH modules function in the opening of promoter DNA and the phosphorylation of Pol II, respectively. The TFIIH core subunit Ssl2 (a homologue of human XPB) is positioned on downstream DNA by the 'E-bridge' helix in TFIIE, consistent with TFIIE-stimulated DNA opening. The TFIIH kinase module subunit Tfb3 (MAT1 in human) anchors the kinase Kin28 (CDK7), which is mobile in the PIC but preferentially located between the Mediator hook and shoulder in the PIC-core Mediator complex. Open spaces between the Mediator head and middle modules may allow access of the kinase to its substrate, the C-terminal domain of Pol II.
A: DNA-directed RNA polymerase II subunit RPB1 B: DNA-directed RNA polymerase II subunit RPB2 C: DNA-directed RNA polymerase II subunit RPB3 D: DNA-directed RNA polymerase II subunit RPB4 E: DNA-directed RNA polymerases I, II, and III subunit RPABC1 F: DNA-directed RNA polymerases I, II, and III subunit RPABC2 G: DNA-directed RNA polymerase II subunit RPB7 H: DNA-directed RNA polymerases I, II, and III subunit RPABC3 I: DNA-directed RNA polymerase II subunit RPB9 J: DNA-directed RNA polymerases I, II, and III subunit RPABC5 K: DNA-directed RNA polymerase II subunit RPB11 L: DNA-directed RNA polymerases I, II, and III subunit RPABC4 M: Transcription initiation factor IIB N: NONTEMPLATE DNA O: TATA-box-binding protein Q: Transcription initiation factor IIF subunit alpha R: Transcription initiation factor IIF subunit beta T: TEMPLATE DNA U: Transcription initiation factor IIA large subunit V: Transcription initiation factor IIA subunit 2 W: Transcription initiation factor IIE subunit alpha X: Transcription initiation factor IIE subunit beta 0: DNA repair helicase RAD3 1: RNA polymerase II transcription factor B subunit 1 2: RNA polymerase II transcription factor B subunit 2 3: RNA polymerase II transcription factor B subunit 3 4: RNA polymerase II transcription factor B subunit 4 5: RNA polymerase II transcription factor B subunit 5 6: Suppressor of stem-loop protein 1 7: DNA repair helicase RAD25 Z: Unknown protein ヘテロ分子
DNA-directed RNA polymerase II subunit ... , 7種, 7分子 ABCDGIK
#1: タンパク質
DNA-directedRNApolymeraseIIsubunitRPB1 / RNA polymerase II subunit B1 / DNA-directed RNA polymerase III largest subunit / RNA polymerase II ...RNA polymerase II subunit B1 / DNA-directed RNA polymerase III largest subunit / RNA polymerase II subunit B220
DNA-directed RNA polymerases I, II, and III subunit ... , 5種, 5分子 EFHJL
#5: タンパク質
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC1 / RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 ...RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 kDa polypeptide
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC2 / RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 ...RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 kDa polypeptide
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC3 / RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I / and III 14.5 kDa polypeptide
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC5 / RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I / and III 8.3 kDa polypeptide
Transcription initiation factor IIF subunit ... , 2種, 2分子 QR
#16: タンパク質
TranscriptioninitiationfactorIIFsubunitalpha / TFIIF-alpha / TFIIF large subunit / Transcription factor G 105 kDa subunit / P105
分子量: 82320.570 Da / 分子数: 1 Mutation: Residues indicated as UNK are of uncertain amino acid register and may deviate from the given register by few amino acids. For details about modelling of the subunits refer to the ...Mutation: Residues indicated as UNK are of uncertain amino acid register and may deviate from the given register by few amino acids. For details about modelling of the subunits refer to the associated publication. Residues indicated as UNK with the chain identifier Z are part of an assembly between the clutch domains of Tfb2 and Ssl2 but could not be assigned to any of the two proteins with confidence since domain swapping/beta-strand exchange may occur. To account for this uncertainty, a theoretical chain Z was introduced for this residue range but not assigned to any of the PIC components. Submission based on experimental data from EMDB EMD-3846. 由来タイプ: 組換発現 由来: (組換発現) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) 遺伝子: TFG1, SSU71, YGR186W, G7526 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: P41895
#17: タンパク質
TranscriptioninitiationfactorIIFsubunitbeta / ATP-dependent helicase TFG2 / TFIIF medium subunit / TFIIF-beta / Transcription factor G 54 kDa subunit
RNApolymeraseIItranscriptionfactorBsubunit3 / RNA polymerase II transcription factor B 38 kDa subunit / RNA polymerase II transcription factor B p38 subunit
分子量: 3677.524 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: Residues indicated as UNK with the chain identifier Z are part of an assembly between the clutch domains of Tfb2 and Ssl2 but could not be assigned to any of the two proteins with confidence ...詳細: Residues indicated as UNK with the chain identifier Z are part of an assembly between the clutch domains of Tfb2 and Ssl2 but could not be assigned to any of the two proteins with confidence since domain swapping/beta-strand exchange may occur. To account for this uncertainty, a theoretical chain Z was introduced for this residue range but not assigned to any of the PIC components. 由来: (組換発現) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) 発現宿主: Trichoplusia ni (イラクサキンウワバ)