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- PDB-5oki: Crystal structure of the Ctf18-1-8 module from Ctf18-RFC in compl... -

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Basic information

Entry
Database: PDB / ID: 5oki
TitleCrystal structure of the Ctf18-1-8 module from Ctf18-RFC in complex with a 63 kDa fragment of DNA Polymerase epsilon
Components
  • Chromosome transmission fidelity protein 18
  • Chromosome transmission fidelity protein 8
  • DNA polymerase epsilon catalytic subunit A
  • Sister chromatid cohesion protein DCC1
KeywordsREPLICATION / Clamp loader DNA-binding protein DNA polymerase Winged-helix domain
Function / homology
Function and homology information


maintenance of mitotic sister chromatid cohesion / gene conversion / DNA replication initiation / epsilon DNA polymerase complex / Ctf18 RFC-like complex / maintenance of DNA trinucleotide repeats / SUMO binding / DNA clamp loader activity / Activation of the pre-replicative complex / single-stranded DNA 3'-5' DNA exonuclease activity ...maintenance of mitotic sister chromatid cohesion / gene conversion / DNA replication initiation / epsilon DNA polymerase complex / Ctf18 RFC-like complex / maintenance of DNA trinucleotide repeats / SUMO binding / DNA clamp loader activity / Activation of the pre-replicative complex / single-stranded DNA 3'-5' DNA exonuclease activity / Termination of translesion DNA synthesis / mitotic DNA replication checkpoint signaling / mitotic intra-S DNA damage checkpoint signaling / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / single-stranded DNA helicase activity / nucleotide-excision repair, DNA gap filling / DNA replication proofreading / mitotic sister chromatid cohesion / leading strand elongation / nuclear replication fork / chromosome, centromeric region / Dual incision in TC-NER / DNA replication initiation / error-prone translesion synthesis / base-excision repair, gap-filling / replication fork / double-strand break repair via homologous recombination / base-excision repair / DNA-templated DNA replication / double-strand break repair via nonhomologous end joining / double-strand break repair / mitotic cell cycle / single-stranded DNA binding / 4 iron, 4 sulfur cluster binding / double-stranded DNA binding / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nucleotide binding / mRNA binding / chromatin / ATP hydrolysis activity / mitochondrion / DNA binding / zinc ion binding / ATP binding / nucleus
Similarity search - Function
Chromosome transmission fidelity protein 8 / Ctf8 / Sister chromatid cohesion protein Dcc1 / Sister chromatid cohesion protein Dcc1 / DNA polymerase epsilon, catalytic subunit A, C-terminal / DNA polymerase epsilon catalytic subunit / Domain of unknown function (DUF1744) / DUF1744 / : / DNA polymerase family B, thumb domain ...Chromosome transmission fidelity protein 8 / Ctf8 / Sister chromatid cohesion protein Dcc1 / Sister chromatid cohesion protein Dcc1 / DNA polymerase epsilon, catalytic subunit A, C-terminal / DNA polymerase epsilon catalytic subunit / Domain of unknown function (DUF1744) / DUF1744 / : / DNA polymerase family B, thumb domain / DNA polymerase family B / DNA polymerase family B, exonuclease domain / DNA-directed DNA polymerase, family B, exonuclease domain / DNA-directed DNA polymerase, family B, multifunctional domain / DNA polymerase, palm domain superfamily / DNA polymerase type-B family / DNA-directed DNA polymerase, family B / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / Ribonuclease H superfamily / Ribonuclease H-like superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
DNA polymerase epsilon catalytic subunit A / Sister chromatid cohesion protein DCC1 / Chromosome transmission fidelity protein 8 / Chromosome transmission fidelity protein 18
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 4.5 Å
AuthorsGrabarczyk, D.B. / Kisker, C.
Funding support Germany, 1items
OrganizationGrant numberCountry
Alexander von Humboldt Foundation Germany
CitationJournal: Structure / Year: 2018
Title: Structural Basis for the Recruitment of Ctf18-RFC to the Replisome.
Authors: Grabarczyk, D.B. / Silkenat, S. / Kisker, C.
History
DepositionJul 25, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 20, 2017Provider: repository / Type: Initial release
Revision 1.1Jan 10, 2018Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA polymerase epsilon catalytic subunit A
B: DNA polymerase epsilon catalytic subunit A
C: Sister chromatid cohesion protein DCC1
D: Chromosome transmission fidelity protein 8
E: Chromosome transmission fidelity protein 18
G: Sister chromatid cohesion protein DCC1
H: Chromosome transmission fidelity protein 8
I: Chromosome transmission fidelity protein 18


Theoretical massNumber of molelcules
Total (without water)244,4898
Polymers244,4898
Non-polymers00
Water00
1
A: DNA polymerase epsilon catalytic subunit A
G: Sister chromatid cohesion protein DCC1
H: Chromosome transmission fidelity protein 8
I: Chromosome transmission fidelity protein 18


Theoretical massNumber of molelcules
Total (without water)122,2444
Polymers122,2444
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: DNA polymerase epsilon catalytic subunit A
C: Sister chromatid cohesion protein DCC1
D: Chromosome transmission fidelity protein 8
E: Chromosome transmission fidelity protein 18


Theoretical massNumber of molelcules
Total (without water)122,2444
Polymers122,2444
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)121.678, 145.826, 378.752
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11chain D
21chain H
12chain E
22chain I
13chain C
23chain G
14chain A
24chain B

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111chain DD2 - 132
211chain HH2 - 132
112chain EE715 - 740
212chain II715 - 740
113chain CC2 - 380
213chain GG2 - 380
114chain AA31 - 524
214chain BB27 - 524

NCS ensembles :
ID
1
2
3
4

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Components

#1: Protein DNA polymerase epsilon catalytic subunit A / DNA polymerase II subunit A


Mass: 59749.184 Da / Num. of mol.: 2 / Mutation: D290A. E292A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: POL2, DUN2, YNL262W, N0825 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P21951, DNA-directed DNA polymerase
#2: Protein Sister chromatid cohesion protein DCC1 / Defective in sister chromatid cohesion protein 1


Mass: 44133.785 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: DCC1, YCL016C, YCL16C / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P25559
#3: Protein Chromosome transmission fidelity protein 8


Mass: 15189.688 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: CTF8, YHR191C / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P38877
#4: Protein/peptide Chromosome transmission fidelity protein 18


Mass: 3171.607 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: CTF18, CHL12, YMR078C, YM9582.03C / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P49956

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.44 Å3/Da / Density % sol: 64.25 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 12% PEG 10000, 0.1 M MES pH 6.5, 0.2 M ammonium chloride

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.918 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 5, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.918 Å / Relative weight: 1
ReflectionResolution: 4.5→47.72 Å / Num. obs: 20450 / % possible obs: 99.9 % / Redundancy: 6.6 % / Biso Wilson estimate: 141.74 Å2 / CC1/2: 0.969 / Rmerge(I) obs: 0.668 / Rpim(I) all: 0.278 / Rrim(I) all: 0.725 / Net I/σ(I): 3.5 / Num. measured all: 135607 / Scaling rejects: 0
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
4.5-4.936.62.5483199048140.3231.0672.7650.8100
11.02-47.725.50.075823914920.9970.0340.08218.998.9

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
Aimless0.5.32data scaling
PDB_EXTRACT3.22data extraction
XDSdata reduction
PHASERphasing
RefinementResolution: 4.5→47.724 Å / SU ML: 0.89 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 37.5 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.3121 1011 4.96 %Random selection
Rwork0.2926 19376 --
obs0.2935 20387 99.84 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 354.28 Å2 / Biso mean: 186.3768 Å2 / Biso min: 65.41 Å2
Refinement stepCycle: final / Resolution: 4.5→47.724 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15910 0 0 0 15910
Num. residues----1967
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00416267
X-RAY DIFFRACTIONf_angle_d1.04321979
X-RAY DIFFRACTIONf_chiral_restr0.0422426
X-RAY DIFFRACTIONf_plane_restr0.0062808
X-RAY DIFFRACTIONf_dihedral_angle_d12.176097
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11D1256X-RAY DIFFRACTION9.22TORSIONAL
12H1256X-RAY DIFFRACTION9.22TORSIONAL
21E236X-RAY DIFFRACTION9.22TORSIONAL
22I236X-RAY DIFFRACTION9.22TORSIONAL
31C3496X-RAY DIFFRACTION9.22TORSIONAL
32G3496X-RAY DIFFRACTION9.22TORSIONAL
41A4197X-RAY DIFFRACTION9.22TORSIONAL
42B4197X-RAY DIFFRACTION9.22TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 7

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
4.5001-4.73710.3871450.357427332878100
4.7371-5.03360.38851580.34927062864100
5.0336-5.42180.38151350.363127282863100
5.4218-5.96650.39941540.362827472901100
5.9665-6.82770.39381330.350227852918100
6.8277-8.59390.29341460.292427862932100
8.5939-47.72710.19961400.20372891303199
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.3764-3.02973.96295.92.76895.9630.50611.1461-1.33710.0239-0.0780.45481.07290.5004-0.42142.5375-0.5422-0.22041.8837-0.04812.0723-59.0926-26.21091.5082
20.47930.4459-0.51420.7428-1.16351.31180.49920.0575-0.20030.5197-0.0008-0.09170.87061.1211-0.32252.8982-0.2476-0.45092.1187-0.68442.4002-56.609-17.6274-4.567
32.71710.01790.20435.8932-0.2582.06720.62720.41880.95571.0301-0.61980.38090.47830.2701-0.00383.105-0.63850.32380.930.11371.7063-46.5458-7.913427.6489
46.6046-2.9385-1.56771.65660.69992.4596-0.608-0.3131-0.96040.33590.61610.49391.518-0.8395-0.12062.2061-0.6008-0.07051.64510.0671.8534-22.49371.860884.4081
5-0.2246-0.402-0.22397.78723.25415.27751.2643-1.51450.0674-0.3803-0.323-1.7765-0.4806-0.78-0.68521.2684-0.18-0.14682.403-0.03272.2347-23.289214.256108.6887
63.2166-0.1051.73596.14730.1595.3141-0.31970.3975-0.3388-1.1310.58080.41060.20970.2254-0.20941.1145-0.42840.07531.4026-0.21381.5063-9.530820.364866.3673
74.89070.03152.22681.2036-0.79923.99530.2381-0.8683-1.12980.01340.3308-1.1306-0.3765-0.0817-0.14831.8818-0.7164-0.52761.7856-0.16242.0072-3.250417.55776.363
81.4445-1.62670.73012.9350.31411.225-0.92660.41210.1543-0.03910.1750.14841.46530.50340.87722.9592-0.4013-0.13121.16450.07731.910623.502415.979329.4172
98.33482.6387-4.34395.9988-3.6954.5412-0.46582.44880.7897-0.92680.06830.8991-1.2293-0.69580.60433.09330.0294-0.59892.22090.31981.98462.903-13.194123.0727
103.95-1.84380.45454.0004-3.7419.9480.71760.2899-1.9308-1.30050.0745-0.25680.4386-0.1331-0.84162.7328-0.30280.08391.19010.0882.11361.8375-29.786132.9135
115.6513-0.81120.2610.0481-0.12930.48010.54310.66971.6372-0.2868-0.4228-0.31690.58732.35230.00844.3207-1.3955-0.50390.5625-0.13121.7492-1.5016-22.521342.2869
122.8265-4.8794.04668.5669-6.98755.74851.50560.0093-1.4122-1.57230.9131.41870.5702-0.8796-1.37664.6692-1.0301-0.20521.76930.1052.3326-22.6118-20.415147.2594
134.56680.1251-0.32690.30750.66493.09890.8345-1.3368-0.6253-0.34340.2786-1.7822-3.4067-1.3569-0.2692.4722-1.2997-0.4021.0705-0.16082.1966-13.0917-15.391245.3954
146.43957.0043-3.80599.7389-7.06596.8606-0.8471.0779-0.314-1.89780.04120.5222.2174-0.62580.5833.16180.0171-0.09881.2034-0.41641.205-14.7825-1.075244.5398
158.03733.7051-3.90021.6974-1.84342.06570.7459-2.4605-0.08020.6863-1.25680.36840.21870.95370.29591.7802-0.2893-0.38261.6639-0.17571.6108-10.55844.837150.1571
161.9936-4.45022.02223.7815-3.0117.26031.4417-0.3944-4.0669-1.23710.90272.61711.4667-0.7181-2.01814.0861-1.09220.9755-1.02940.91362.626520.69932.095123.3081
174.55331.6856-2.41527.4014-4.88561.99950.99880.45682.0312-2.82490.8010.4391-0.4334-0.2211-1.36655.6227-0.3641-0.70951.1886-0.42473.334334.93634.9530.594
181.98486.6632-1.12124.681-6.27281.98941.35771.1030.63-2.84030.05670.7955-0.720.2679-1.10232.7358-0.0103-0.64742.00930.41722.179137.130914.879438.1695
192.5118-2.87521.66874.367-1.54781.535-0.04291.8228-0.7701-0.3866-0.7095-1.20620.7197-0.2646-0.09083.2045-0.38020.4610.59640.12912.128325.410529.618528.0962
200.2684-0.5621-0.15161.24720.2790.0611.2211.4412-0.0052-1.23420.54971.32660.5074-0.1515-0.87013.1746-1.1741-1.41034.0392-0.27932.753834.949841.572116.0474
212.0115-8.3458-6.42564.69354.85882.00620.68132.26050.1256-1.9786-2.24181.504-1.3225-1.11311.3822.7833-1.0557-1.05253.5472-0.12971.151235.224130.213915.5447
222.06340.05260.59452.39294.79099.66950.1329-0.46211.0008-0.94490.94740.05862.89930.8982-0.51382.1848-1.1989-0.27981.6198-0.35091.875633.616135.648436.0255
230.05050.4350.18482.00265.1762.47321.03450.7250.0031.9827-1.1038-1.21710.5497-1.1852-0.06042.2456-0.58680.17261.76360.72821.04228.048232.850940.891
246.7949-2.4927-2.07373.2124.57032.0107-0.20160.3491-2.5678-1.40250.3031-0.31330.4174-0.83470.02393.1934-0.13950.61351.16940.13512.335423.965611.737626.6917
255.18-1.8274-1.6091.87181.67071.6114-1.16470.4914-0.4567-0.8634-0.42582.00471.1460.27931.18043.28230.3998-0.77470.60060.54361.89225.507833.028139.4882
261.99882.0036-2.75772.0003-2.140.3661-3.19866.65132.8302-4.29653.2930.35930.5285-0.9273-0.61771.1503-0.4559-0.06791.8387-0.00161.196826.464945.208924.5744
271.99839.09184.18981.9975-1.28375.98681.63370.0592.07080.8619-0.29721.4849-1.7136-3.6071-1.60361.26680.54742.07151.99570.91862.392516.464138.603133.884
286.76537.4537-0.91598.0845-0.97760.1094-1.0229-0.32430.00441.75260.69471.5854-0.86220.20440.52493.7762-0.13150.51371.1486-0.58822.35557.266320.807733.996
299.35839.5059-1.45872.00222.04942.0045-0.3368-0.48481.08210.2255-0.1664-0.91841.1681-0.31120.36542.5629-0.2639-0.38672.0164-0.48321.848811.336110.963420.0059
303.0741.6229-2.19371.2809-1.15372.1265-0.90431.92372.94051.90171.68591.43010.0625-2.60940.23941.4589-1.5538-0.7766-1.12951.0282.5919-61.18124.175864.2732
316.51740.79311.130.29820.82952.9019-0.0153-0.09451.19140.35690.63740.5455-0.18850.1927-0.50981.2374-0.1605-0.45011.45380.58482.769-53.913424.08964.7638
324.8617-2.33842.31885.82340.86355.3034-1.6773-0.88670.58240.81430.71970.77580.4411-1.62220.75021.7502-0.8044-0.45891.69040.24462.7898-91.0953-5.007364.2357
332.0008-6.37387.47396.1616-5.58922.0101-0.194-1.5342-2.7497-1.51590.77683.35953.263-1.1529-0.18362.8303-0.3620.44632.09430.63012.7415-85.6663-28.227647.4308
341.3973-1.1891-1.79822.08460.86991.00220.1285-0.28751.7837-1.2324-0.66550.8064-0.0150.76340.12232.1541-1.07710.25292.26160.27611.9335-82.1088-18.631849.0176
350.1053-0.0702-0.12810.11530.1230.17510.66640.2458-0.23751.5450.2824-0.8148-0.05760.6704-0.10591.436-0.6511.20973.61730.58131.9619-67.3234-18.665349.1643
368.08843.85866.6745.0958-1.31621.9267-1.0604-0.56063.6088-0.1127-1.70160.3970.21791.03522.40531.7066-0.215-0.02173.1861-0.13132.26-63.821-12.686843.8813
372.00321.2472-2.54612.99552.96944.3384-0.17040.16580.5797-0.07070.3441.1795-0.3048-0.7511-0.433-1.2431.5370.47212.48021.24914.0589-70.35317.925470.9017
380.4876-0.17590.84910.28241.04769.8858-0.47010.3201-0.8494-0.4564-0.01640.34571.4191-2.12920.4931.1555-1.3068-0.91512.46950.09224.9905-70.012732.196663.8153
391.46790.39172.04920.09090.5872.85760.20331.34992.59710.0920.5349-0.3905-1.9857-0.43120.1110.8715-2.0944-0.77741.9640.62972.9454-56.824233.556658.6053
408.0335-7.23658.13116.6006-7.38768.2165-0.7119-1.82151.46081.90390.35820.3744-0.8211-1.62120.69272.2937-0.7806-0.77111.434-0.57020.7196-35.997225.074570.5148
416.9849-0.09392.17729.77046.36295.76181.0176-0.3006-3.3261-2.31852.1013-0.95130.034-0.4994-3.01653.79820.1374-1.79152.9603-0.54752.8352-34.448239.824878.6283
426.63486.1918-3.71947.3399-1.45384.7168-0.21050.8586-0.34110.51780.66941.6905-0.02190.1134-0.07443.23-0.5391-1.75561.8984-0.11944.0829-45.173637.301878.8024
433.4472-0.3945-6.37742.4168-0.27581.9710.01260.03480.5104-0.1996-0.21921.1955-0.846-1.52210.10941.113-3.0147-0.8641-1.17641.96233.1043-41.144536.64557.0165
442.05793.01444.25431.9615-1.37641.9689-0.93661.01352.0108-1.13212.57531.2277-0.0311-1.326-0.47792.7036-0.0563-1.7741.31082.1023.2663-38.662732.451955.9709
453.4079-0.8983-5.57762.81912.3092.0017-1.2035-0.85880.29420.53560.7270.8431-0.4096-0.05850.34751.133-1.2096-0.7491.3355-0.16912.6505-59.135422.017963.0627
460.83791.85620.31293.5753-0.15321.4172-0.41460.45431.1012-0.1573-0.69231.1976-1.19990.12490.31311.9868-0.6334-0.83551.86310.18362.5704-51.305525.965762.3265
472.03755.5537-4.71241.9899-3.99082.09990.26050.2113-0.463.97770.21520.2952-1.729-0.0615-0.60372.6144-0.04570.35431.3172-0.14361.9998-31.38625.837764.7348
481.98566.39515.94142.03792.53371.9862-1.3841.62642.11330.18852.72321.9975-1.3081.5184-1.19092.1653-0.31940.03031.5476-0.0061.9155-49.46358.493659.2468
499.13776.9766-4.47877.042-6.94099.3660.4651-1.08421.70983.44240.42932.792-1.4134-1.9425-1.05711.6716-0.48930.61391.48161.34141.4253-59.64069.7373.8748
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 31 through 212 )A31 - 212
2X-RAY DIFFRACTION2chain 'A' and (resid 213 through 274 )A213 - 274
3X-RAY DIFFRACTION3chain 'A' and (resid 275 through 524 )A275 - 524
4X-RAY DIFFRACTION4chain 'B' and (resid 27 through 145 )B27 - 145
5X-RAY DIFFRACTION5chain 'B' and (resid 146 through 248 )B146 - 248
6X-RAY DIFFRACTION6chain 'B' and (resid 249 through 466 )B249 - 466
7X-RAY DIFFRACTION7chain 'B' and (resid 467 through 524 )B467 - 524
8X-RAY DIFFRACTION8chain 'C' and (resid 2 through 112 )C2 - 112
9X-RAY DIFFRACTION9chain 'C' and (resid 113 through 167 )C113 - 167
10X-RAY DIFFRACTION10chain 'C' and (resid 168 through 241 )C168 - 241
11X-RAY DIFFRACTION11chain 'C' and (resid 242 through 268 )C242 - 268
12X-RAY DIFFRACTION12chain 'C' and (resid 269 through 286 )C269 - 286
13X-RAY DIFFRACTION13chain 'C' and (resid 287 through 324 )C287 - 324
14X-RAY DIFFRACTION14chain 'C' and (resid 325 through 350 )C325 - 350
15X-RAY DIFFRACTION15chain 'C' and (resid 351 through 380 )C351 - 380
16X-RAY DIFFRACTION16chain 'D' and (resid 2 through 8 )D2 - 8
17X-RAY DIFFRACTION17chain 'D' and (resid 9 through 16 )D9 - 16
18X-RAY DIFFRACTION18chain 'D' and (resid 17 through 26 )D17 - 26
19X-RAY DIFFRACTION19chain 'D' and (resid 27 through 44 )D27 - 44
20X-RAY DIFFRACTION20chain 'D' and (resid 45 through 58 )D45 - 58
21X-RAY DIFFRACTION21chain 'D' and (resid 59 through 68 )D59 - 68
22X-RAY DIFFRACTION22chain 'D' and (resid 69 through 87 )D69 - 87
23X-RAY DIFFRACTION23chain 'D' and (resid 88 through 96 )D88 - 96
24X-RAY DIFFRACTION24chain 'D' and (resid 97 through 119 )D97 - 119
25X-RAY DIFFRACTION25chain 'D' and (resid 120 through 132 )D120 - 132
26X-RAY DIFFRACTION26chain 'E' and (resid 715 through 719 )E715 - 719
27X-RAY DIFFRACTION27chain 'E' and (resid 720 through 724 )E720 - 724
28X-RAY DIFFRACTION28chain 'E' and (resid 725 through 734 )E725 - 734
29X-RAY DIFFRACTION29chain 'E' and (resid 735 through 740 )E735 - 740
30X-RAY DIFFRACTION30chain 'G' and (resid 2 through 37 )G2 - 37
31X-RAY DIFFRACTION31chain 'G' and (resid 38 through 112 )G38 - 112
32X-RAY DIFFRACTION32chain 'G' and (resid 113 through 268 )G113 - 268
33X-RAY DIFFRACTION33chain 'G' and (resid 269 through 286 )G269 - 286
34X-RAY DIFFRACTION34chain 'G' and (resid 287 through 324 )G287 - 324
35X-RAY DIFFRACTION35chain 'G' and (resid 325 through 348 )G325 - 348
36X-RAY DIFFRACTION36chain 'G' and (resid 349 through 380 )G349 - 380
37X-RAY DIFFRACTION37chain 'H' and (resid 2 through 8 )H2 - 8
38X-RAY DIFFRACTION38chain 'H' and (resid 9 through 16 )H9 - 16
39X-RAY DIFFRACTION39chain 'H' and (resid 17 through 35 )H17 - 35
40X-RAY DIFFRACTION40chain 'H' and (resid 36 through 44 )H36 - 44
41X-RAY DIFFRACTION41chain 'H' and (resid 45 through 58 )H45 - 58
42X-RAY DIFFRACTION42chain 'H' and (resid 59 through 68 )H59 - 68
43X-RAY DIFFRACTION43chain 'H' and (resid 69 through 84 )H69 - 84
44X-RAY DIFFRACTION44chain 'H' and (resid 85 through 96 )H85 - 96
45X-RAY DIFFRACTION45chain 'H' and (resid 97 through 107 )H97 - 107
46X-RAY DIFFRACTION46chain 'H' and (resid 108 through 132 )H108 - 132
47X-RAY DIFFRACTION47chain 'I' and (resid 715 through 724 )I715 - 724
48X-RAY DIFFRACTION48chain 'I' and (resid 725 through 734 )I725 - 734
49X-RAY DIFFRACTION49chain 'I' and (resid 735 through 740 )I735 - 740

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