+Open data
-Basic information
Entry | Database: PDB / ID: 5obz | |||||||||
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Title | low resolution structure of the p34ct/p44ct minimal complex | |||||||||
Components | (Putative transcription factor) x 2 | |||||||||
Keywords | TRANSCRIPTION / complex | |||||||||
Function / homology | Function and homology information transcription factor TFIIH holo complex / transcription factor TFIIH core complex / nucleotide-excision repair / DNA-templated transcription / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / zinc ion binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Chaetomium thermophilum (fungus) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.7 Å | |||||||||
Authors | Schoenwetter, E. / Koelmel, W. / Schmitt, D.R. / Kuper, J. / Kisker, C. | |||||||||
Funding support | Germany, 2items
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Citation | Journal: Nucleic Acids Res. / Year: 2017 Title: The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH. Authors: Radu, L. / Schoenwetter, E. / Braun, C. / Marcoux, J. / Koelmel, W. / Schmitt, D.R. / Kuper, J. / Cianferani, S. / Egly, J.M. / Poterszman, A. / Kisker, C. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5obz.cif.gz | 71.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5obz.ent.gz | 50 KB | Display | PDB format |
PDBx/mmJSON format | 5obz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ob/5obz ftp://data.pdbj.org/pub/pdb/validation_reports/ob/5obz | HTTPS FTP |
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-Related structure data
Related structure data | 5nusSC 5o85C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31972.957 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Gene: CTHT_0004460 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL / References: UniProt: G0RXV8 | ||
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#2: Protein | Mass: 17850.045 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Gene: CTHT_0002690 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL / References: UniProt: G0RZE6 | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.27 Å3/Da / Density % sol: 62.38 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / Details: 15% PEG 20,000 100 mM MES pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 11, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 3.7→45.2 Å / Num. obs: 6120 / % possible obs: 99.9 % / Redundancy: 8.1 % / Rmerge(I) obs: 0.2 / Net I/σ(I): 9.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5NUS Resolution: 3.7→45.2 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.945 / SU B: 39.081 / SU ML: 0.506 / Cross valid method: THROUGHOUT / ESU R Free: 0.553 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 152.72 Å2
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Refinement step | Cycle: 1 / Resolution: 3.7→45.2 Å
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Refine LS restraints |
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