[English] 日本語
![](img/lk-miru.gif)
- PDB-5oa5: CELLOBIOHYDROLASE I (CEL7A) FROM HYPOCREA JECORINA WITH IMPROVED ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 5oa5 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | CELLOBIOHYDROLASE I (CEL7A) FROM HYPOCREA JECORINA WITH IMPROVED THERMAL STABILITY | |||||||||
![]() | Exoglucanase 1 | |||||||||
![]() | HYDROLASE / CELLULASE / PROTEIN ENGINEERING | |||||||||
Function / homology | ![]() cellulose 1,4-beta-cellobiosidase (non-reducing end) / cellulose 1,4-beta-cellobiosidase activity / cellulose binding / cellulose catabolic process / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Goedegebuur, F. / Hansson, H. / Karkehabadi, S. / Mikkelsen, N. / Stahlberg, J. / Sandgren, M. | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution. Authors: Goedegebuur, F. / Dankmeyer, L. / Gualfetti, P. / Karkehabadi, S. / Hansson, H. / Jana, S. / Huynh, V. / Kelemen, B.R. / Kruithof, P. / Larenas, E.A. / Teunissen, P.J.M. / Stahlberg, J. / ...Authors: Goedegebuur, F. / Dankmeyer, L. / Gualfetti, P. / Karkehabadi, S. / Hansson, H. / Jana, S. / Huynh, V. / Kelemen, B.R. / Kruithof, P. / Larenas, E.A. / Teunissen, P.J.M. / Stahlberg, J. / Payne, C.M. / Mitchinson, C. / Sandgren, M. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 186.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 146.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 476.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 478.6 KB | Display | |
Data in XML | ![]() | 36.7 KB | Display | |
Data in CIF | ![]() | 53.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2v3iS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 46262.238 Da / Num. of mol.: 2 / Fragment: CATALYTIC DOMAIN, RESIDUES 18-451 Mutation: S8P, T41I, N49S, A68T, N89D, S92T, S113N, S196T, P227L, D249K, T255P, S278P, E295K, T296P, T332Y, V304D, S411F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P62694, cellulose 1,4-beta-cellobiosidase (non-reducing end) #2: Sugar | ChemComp-NAG / #3: Chemical | ChemComp-GOL / | #4: Water | ChemComp-HOH / | Sequence details | ENGINEERED | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.45 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 25.5% POLYETHYLENE GLYCOL (PEG) 4000, 0.17 M AMSO4 AND 15% GLYCEROL |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 5, 2012 / Details: PT COATED MIRRORS |
Radiation | Monochromator: HORIZONTALLY DIFFRACTING SI (111) MONOCHROMATOR Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 1.99→41.16 Å / Num. obs: 56990 / % possible obs: 99.6 % / Observed criterion σ(I): 2 / Redundancy: 3.71 % / Rmerge(I) obs: 0.13 / Net I/σ(I): 4.9 |
Reflection shell | Resolution: 2.1→2.21 Å / Redundancy: 3.73 % / Rmerge(I) obs: 0.41 / Mean I/σ(I) obs: 1.86 / % possible all: 99.8 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2V3I Resolution: 2.1→41.16 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.926 / SU B: 4.952 / SU ML: 0.13 / Cross valid method: THROUGHOUT / ESU R: 0.226 / ESU R Free: 0.19 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.9 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→41.16 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|