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Yorodumi- PDB-5o33: A structure of the GEF Kalirin DH1 domain in complex with the sma... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5o33 | ||||||
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Title | A structure of the GEF Kalirin DH1 domain in complex with the small GTPase Rac1 | ||||||
Components |
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Keywords | HYDROLASE / Complex. GEF. Small GTPase | ||||||
Function / homology | Function and homology information RHOG GTPase cycle / NRAGE signals death through JNK / G alpha (12/13) signalling events / MAPK6/MAPK4 signaling / RAC1 GTPase cycle / maternal process involved in parturition / RHOA GTPase cycle / regulation of respiratory burst / modification of postsynaptic actin cytoskeleton / negative regulation of interleukin-23 production ...RHOG GTPase cycle / NRAGE signals death through JNK / G alpha (12/13) signalling events / MAPK6/MAPK4 signaling / RAC1 GTPase cycle / maternal process involved in parturition / RHOA GTPase cycle / regulation of respiratory burst / modification of postsynaptic actin cytoskeleton / negative regulation of interleukin-23 production / regulation of neutrophil migration / regulation of dendrite development / localization within membrane / NMDA selective glutamate receptor signaling pathway / Activated NTRK2 signals through CDK5 / negative regulation of growth hormone secretion / NADPH oxidase complex / negative regulation of receptor-mediated endocytosis / regulation of hydrogen peroxide metabolic process / ruffle assembly / NTRK2 activates RAC1 / engulfment of apoptotic cell / Inactivation of CDC42 and RAC1 / positive regulation of dendritic spine morphogenesis / WNT5:FZD7-mediated leishmania damping / EPHB-mediated forward signaling / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / regulation of modification of postsynaptic actin cytoskeleton / cortical cytoskeleton organization / habituation / respiratory burst / hepatocyte growth factor receptor signaling pathway / maternal behavior / ruffle organization / cell projection assembly / thioesterase binding / negative regulation of fibroblast migration / regulation of stress fiber assembly / RHO GTPases activate CIT / Nef and signal transduction / sphingosine-1-phosphate receptor signaling pathway / regulation of nitric oxide biosynthetic process / PCP/CE pathway / G alpha (q) signalling events / motor neuron axon guidance / RHO GTPases activate KTN1 / regulation of lamellipodium assembly / positive regulation of neutrophil chemotaxis / Azathioprine ADME / Activation of RAC1 / DCC mediated attractive signaling / positive regulation of cell-substrate adhesion / MET activates RAP1 and RAC1 / neurotransmitter receptor localization to postsynaptic specialization membrane / Wnt signaling pathway, planar cell polarity pathway / Sema4D mediated inhibition of cell attachment and migration / CD28 dependent Vav1 pathway / Ephrin signaling / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / lamellipodium assembly / positive regulation of Rho protein signal transduction / establishment or maintenance of cell polarity / regulation of cell size / DSCAM interactions / extrinsic component of membrane / neuromuscular junction development / Activation of RAC1 downstream of NMDARs / small GTPase-mediated signal transduction / Rho GDP-dissociation inhibitor binding / NRAGE signals death through JNK / Rac protein signal transduction / RHO GTPases activate PAKs / positive regulation of focal adhesion assembly / social behavior / semaphorin-plexin signaling pathway / ficolin-1-rich granule membrane / Sema3A PAK dependent Axon repulsion / EPH-ephrin mediated repulsion of cells / RHO GTPases Activate NADPH Oxidases / RHO GTPases Activate WASPs and WAVEs / anatomical structure morphogenesis / RHO GTPases activate IQGAPs / localization / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / positive regulation of lamellipodium assembly / positive regulation of substrate adhesion-dependent cell spreading / regulation of cell migration / positive regulation of microtubule polymerization / RHO GTPases activate PKNs / positive regulation of stress fiber assembly / GPVI-mediated activation cascade / RAC1 GTPase cycle / EPHB-mediated forward signaling / actin filament polymerization / lactation / cell-matrix adhesion / axonogenesis / cell chemotaxis / adult locomotory behavior / substrate adhesion-dependent cell spreading Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.64 Å | ||||||
Authors | Gray, J. / Krojer, T. / Talon, R. / Fairhead, M. / Bountra, C. / Arrowsmith, C.H. / Edwards, A. / Brennan, P. / von Delft, F. | ||||||
Citation | Journal: To Be Published Title: A structure of the GEF Kalirin DH1 domain in complex with the small GTPase Rac1 Authors: Gray, J. / Krojer, T. / Talon, R. / Fairhead, M. / Bountra, C. / Arrowsmith, C.H. / Edwards, A. / Brennan, P. / von Delft, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5o33.cif.gz | 168.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5o33.ent.gz | 131.8 KB | Display | PDB format |
PDBx/mmJSON format | 5o33.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/o3/5o33 ftp://data.pdbj.org/pub/pdb/validation_reports/o3/5o33 | HTTPS FTP |
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-Related structure data
Related structure data | 2nz8S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19710.764 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAC1, TC25, MIG5 / Production host: Escherichia coli (E. coli) / References: UniProt: P63000 | ||||
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#2: Protein | Mass: 21056.195 Da / Num. of mol.: 1 / Fragment: UNP residues 1253-1432 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Kalrn, Duo, Hapip / Production host: Escherichia coli (E. coli) References: UniProt: P97924, non-specific serine/threonine protein kinase | ||||
#3: Chemical | ChemComp-EDO / #4: Chemical | ChemComp-GDP / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.37 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.2 Details: 0.1M citrate pH4.2, 0.2M sodium chloride, 18% (W/V) PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92819 Å |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Apr 11, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92819 Å / Relative weight: 1 |
Reflection | Resolution: 1.64→57.78 Å / Num. obs: 52137 / % possible obs: 100 % / Redundancy: 18.8 % / Rmerge(I) obs: 0.0971 / Net I/σ(I): 11.21 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2NZ8 Resolution: 1.64→54.78 Å / SU ML: 0.29 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 31.48
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.64→54.78 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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