+Open data
-Basic information
Entry | Database: PDB / ID: 5no8 | ||||||
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Title | Polysaccharide Lyase BACCELL_00875 | ||||||
Components | BACCELL_00875 | ||||||
Keywords | LYASE / Polysaccharide Lyase / Arabinogalactan / L-Rhamnose / Glucuronic Acid | ||||||
Function / homology | Six-hairpin glycosidase superfamily / carbohydrate metabolic process / Six-hairpin glycosidase Function and homology information | ||||||
Biological species | Bacteroides cellulosilyticus DSM 14838 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Cartmell, A. / Munoz-Munoz, J. / Terrapon, N. / Basle, A. / Henrissat, B. / Gilbert, H.J. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: J. Biol. Chem. / Year: 2017 Title: An evolutionarily distinct family of polysaccharide lyases removes rhamnose capping of complex arabinogalactan proteins. Authors: Munoz-Munoz, J. / Cartmell, A. / Terrapon, N. / Basle, A. / Henrissat, B. / Gilbert, H.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5no8.cif.gz | 296.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5no8.ent.gz | 246.7 KB | Display | PDB format |
PDBx/mmJSON format | 5no8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/no/5no8 ftp://data.pdbj.org/pub/pdb/validation_reports/no/5no8 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 79956.438 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacteroides cellulosilyticus DSM 14838 (bacteria) Gene: BACCELL_00875 / Production host: Escherichia coli (E. coli) / References: UniProt: E2N9D0 #2: Chemical | ChemComp-GOL / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.17 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 25% PEG1500, 0.1 M MMT buffer, pH 4.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.98 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jul 31, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→45.45 Å / Num. obs: 177287 / % possible obs: 100 % / Redundancy: 8.4 % / Net I/σ(I): 10.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.7→45.45 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.96 / SU B: 1.925 / SU ML: 0.061 / Cross valid method: THROUGHOUT / ESU R: 0.087 / ESU R Free: 0.084 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.915 Å2
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Refinement step | Cycle: 1 / Resolution: 1.7→45.45 Å
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Refine LS restraints |
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