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- PDB-5nh7: Crystal structure of xylose isomerase from Piromyces E2 in comple... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5nh7 | |||||||||
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Title | Crystal structure of xylose isomerase from Piromyces E2 in complex with two Mg2+ ions and xylose | |||||||||
![]() | Xylose isomerase | |||||||||
![]() | ISOMERASE / TIM-barrel | |||||||||
Function / homology | ![]() xylose catabolic process to ethanol / xylose isomerase / xylose isomerase activity / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Rozeboom, H.J. / Janssen, D.B. | |||||||||
![]() | ![]() Title: Metal Dependence of the Xylose Isomerase from Piromyces sp. E2 Explored by Activity Profiling and Protein Crystallography. Authors: Lee, M. / Rozeboom, H.J. / de Waal, P.P. / de Jong, R.M. / Dudek, H.M. / Janssen, D.B. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 711.3 KB | Display | ![]() |
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PDB format | ![]() | 588.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 509.6 KB | Display | ![]() |
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Full document | ![]() | 518.8 KB | Display | |
Data in XML | ![]() | 77.7 KB | Display | |
Data in CIF | ![]() | 118.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5nh4C ![]() 5nh5C ![]() 5nh6C ![]() 5nh8C ![]() 5nh9C ![]() 5nhaC ![]() 5nhbC ![]() 5nhcC ![]() 5nhdC ![]() 5nheC ![]() 5nhmC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Refine code: _
NCS ensembles :
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Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 49458.672 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Details (production host): pBR322 Ori and ampicillin resistance gene Production host: ![]() ![]() |
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-Sugars , 3 types, 18 molecules ![](data/chem/img/XLS.gif)
![](data/chem/img/XYP.gif)
![](data/chem/img/XYS.gif)
![](data/chem/img/XYP.gif)
![](data/chem/img/XYS.gif)
#3: Sugar | ChemComp-XLS / #4: Sugar | ChemComp-XYP / #5: Sugar | ChemComp-XYS / |
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-Non-polymers , 3 types, 1790 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-MG / #6: Chemical | ChemComp-SO4 / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 45 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 13-15 % PEG3350, 10 mM MgCl2, 0.1 M Hepes pH 7.0 |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 3, 2014 |
Radiation | Monochromator: Helios MX mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→39.5 Å / Num. obs: 125669 / % possible obs: 92 % / Redundancy: 2 % / Biso Wilson estimate: 1.6 Å2 / CC1/2: 0.985 / Rmerge(I) obs: 0.093 / Rpim(I) all: 0.089 / Net I/σ(I): 6.1 |
Reflection shell | Resolution: 1.9→1.93 Å / Redundancy: 2 % / Rmerge(I) obs: 0.354 / Mean I/σ(I) obs: 2 / Num. unique obs: 5765 / CC1/2: 0.746 / Rpim(I) all: 0.338 / % possible all: 84.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: D_1200004044 Resolution: 1.9→39.5 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.946 / SU B: 6.114 / SU ML: 0.094 / Cross valid method: THROUGHOUT / ESU R: 0.163 / ESU R Free: 0.128 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.51 Å2
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Refinement step | Cycle: 1 / Resolution: 1.9→39.5 Å
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Refine LS restraints |
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