Angiopoietin-1receptor / Endothelial tyrosine kinase / Tunica interna endothelial cell kinase / Tyrosine kinase with Ig and ...Endothelial tyrosine kinase / Tunica interna endothelial cell kinase / Tyrosine kinase with Ig and EGF homology domains-2 / Tyrosine-protein kinase receptor TEK / Tyrosine-protein kinase receptor TIE-2 / hTIE2 / p140 TEK
分子量: 37583.312 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: In addition to the Tie2 Fn-like domains, the entity includes mellitin signal peptide, a N-terminal cloning artefact (ADP), C-terminal Factor Xa cleavage site (IEGR) and His-tag. The first Fn- ...詳細: In addition to the Tie2 Fn-like domains, the entity includes mellitin signal peptide, a N-terminal cloning artefact (ADP), C-terminal Factor Xa cleavage site (IEGR) and His-tag. The first Fn-like domain was not in the crystals apparently due to proteolytic removal prior crystallization. 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TEK, TIE2, VMCM, VMCM1 発現宿主: Spodoptera aff. frugiperda 2 RZ-2014 (蝶・蛾) 参照: UniProt: Q02763, receptor protein-tyrosine kinase
モノクロメーター: horizontally side diffracting Silicon 111 crystal プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9762 Å / 相対比: 1
反射
Biso Wilson estimate: 104.1 Å2
反射 シェル
解像度: 2.6→2.76 Å / 冗長度: 5.5 % / Mean I/σ(I) obs: 1.8 / Rsym value: 0.873 / % possible all: 93.6
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解析
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名称
バージョン
分類
PHENIX
1.9_1692
精密化
XDS
データ削減
XSCALE
データスケーリング
autoSHARP
位相決定
精密化
構造決定の手法: 分子置換 開始モデル: CRYSTAL STRUCTURE OF TIE2 FN-LIKE DOMAINS IN SPACE GROUP C2 解像度: 2.6→39.8 Å / SU ML: 0.4 / 交差検証法: FREE R-VALUE / σ(F): 1.36 / 位相誤差: 33.66