PDB-3m6a: Crystal structure of Bacillus subtilis Lon C-terminal domain

ID or keywords:

Entry

Database: PDB / ID: 3m6a

Title

Crystal structure of Bacillus subtilis Lon C-terminal domain

Components

ATP-dependent protease La 1

Endopeptidase La

Keywords

HYDROLASE / alpha / beta / ATP-binding / nucleotide-binding /

protease /

serine protease /

stress response

Function / homology

Function and homology information

endopeptidase La / ATP-dependent peptidase activity / protein quality control for misfolded or incompletely synthesized proteins / cellular response to heat / sequence-specific DNA binding / serine-type endopeptidase activity /

ATP hydrolysis activity /

ATP binding /

cytoplasm
Similarity search - Function

Biological species

Bacillus subtilis (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

SAD / Resolution: 3.4 Å

Authors

Duman, R.E. / Lowe, J.Y.

Citation

Journal: J.Mol.Biol. / Year: 2010
Title: Crystal Structures of Bacillus subtilis Lon Protease.
Authors: Duman, R.E. / Lowe, J.

History

Deposition	Mar 15, 2010	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jun 30, 2010	Provider: repository / Type: Initial release
Revision 1.1	Jul 13, 2011	Group: Version format compliance
Revision 1.2	Feb 21, 2024	Group: Data collection / Database references / Derived calculations Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_ref_seq_dif / struct_site Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	3m6a.cif.gz	1.1 MB	Display	PDBx/mmCIF format
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PDBx/mmJSON format	3m6a.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/m6/3m6a ftp://data.pdbj.org/pub/pdb/validation_reports/m6/3m6a	HTTPS FTP

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Related structure data

Related structure data	3m65C C: citing same article (ref.)
Similar structure data	6u5z-d 6v11-d 21009 4dx7-d 5jmn-d 6q4o-d 3371 2522 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Related structure data

3m65C

C: citing same article (ref.)

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#116 - Aug 2009 Sulfotransferases similarity (1)

Deposited unit

A: ATP-dependent protease La 1

B: ATP-dependent protease La 1

C: ATP-dependent protease La 1

D: ATP-dependent protease La 1

E: ATP-dependent protease La 1

F: ATP-dependent protease La 1

hetero molecules

363 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author

2

A: ATP-dependent protease La 1

hetero molecules

defined by software
60.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

B: ATP-dependent protease La 1

hetero molecules

defined by software
60.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

C: ATP-dependent protease La 1

hetero molecules

defined by software
60.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

5

D: ATP-dependent protease La 1

hetero molecules

defined by software
60.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

6

E: ATP-dependent protease La 1

hetero molecules

defined by software
60.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

7

F: ATP-dependent protease La 1

hetero molecules

defined by software
60.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	103.382, 127.400, 148.993
Angle α, β, γ (deg.)	90.00, 100.50, 90.00
Int Tables number	4
Space group name H-M	P12₁1

Noncrystallographic symmetry (NCS)

NCS domain:
NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Selection details
1	1	1	chain A and (resseq 246:298 )
2	1	1	chain B and (resseq 246:298 )
3	1	1	chain C and (resseq 246:298 )
4	1	1	chain D and (resseq 246:298 )
5	1	1	chain E and (resseq 246:298 )
6	1	1	chain F and (resseq 246:298 )
1	1	2	chain A and (resseq 308:382 or resseq 403:427 or resseq 437:490 )
2	1	2	chain B and (resseq 308:382 or resseq 403:427 or resseq 437:490 )
3	1	2	chain C and (resseq 308:382 or resseq 403:427 or resseq 437:490 )
4	1	2	chain D and (resseq 308:382 or resseq 403:427 or resseq 437:490 )
5	1	2	chain E and (resseq 308:382 or resseq 403:427 or resseq 437:490 )
6	1	2	chain F and (resseq 308:382 or resseq 403:427 or resseq 437:490 )
1	1	3	chain A and (resseq 491:582 )
2	1	3	chain B and (resseq 491:582 )
3	1	3	chain C and (resseq 491:582 )
4	1	3	chain D and (resseq 491:582 )
5	1	3	chain E and (resseq 491:582 )
6	1	3	chain F and (resseq 491:582 )
1	1	4	chain A and (resseq 591:770 )
2	1	4	chain B and (resseq 591:770 )
3	1	4	chain C and (resseq 591:770 )
4	1	4	chain D and (resseq 591:770 )
5	1	4	chain E and (resseq 591:770 )
6	1	4	chain F and (resseq 591:770 )

NCS ensembles :

ID
1
2
3
4

#1: Protein	ATP-dependent protease La 1 / Endopeptidase La Mass: 60038.691 Da / Num. of mol.: 6 / Fragment: C-terminal domain (240-774) / Mutation: S677A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Strain: 168 / Gene: BSU28200, lon, lonA / Plasmid: pET28a+ / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(AI) / References: UniProt: P37945, endopeptidase La
#2: Chemical	ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / Adenosine diphosphate Mass: 427.201 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₀P₂ / Comment: ADP, energy-carrying molecule*YM

#1: Protein

ATP-dependent protease La 1 /

Endopeptidase La

Mass: 60038.691 Da / Num. of mol.: 6 / Fragment: C-terminal domain (240-774) / Mutation: S677A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Bacillus subtilis (bacteria) / Strain: 168 / Gene: BSU28200, lon, lonA / Plasmid: pET28a+ / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21(AI) / References: UniProt: P37945,

endopeptidase La

#2: Chemical

ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE /

Adenosine diphosphate

Mass: 427.201 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₀P₂ / Comment: ADP, energy-carrying molecule*YM

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.76 Å³/Da / Density % sol: 55.36 %
Crystal grow	Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 0.1M Sodium Citrate, 0.5M Ammonium Acetate, 14% PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

ESRF

/ Beamline: ID23-1 / Wavelength: 0.9537,1.2546

Detector

Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 4, 2008

Radiation

Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

ID	Wavelength (Å)	Relative weight
1	0.9537	1
2	1.2546	1

Reflection

Resolution: 3.4→58.42 Å / Num. all: 52274 / Num. obs: 52431 / % possible obs: 99.7 % / Observed criterion σ(F): 1.3 / Observed criterion σ(I): 1 / Redundancy: 3.5 % / R_merge(I) obs: 0.089 / R_sym value: 0.105 / Net I/σ(I): 8

Reflection shell

Resolution: 3.4→3.58 Å / Redundancy: 3.6 % / R_merge(I) obs: 0.578 / Mean I/σ(I) obs: 2.3 / R_sym value: 0.68 / % possible all: 100

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Processing

Software

Name	Version	Classification
DNA		data collection
PHASES		phasing
PHENIX	(phenix.refine: dev_249)	refinement
MOSFLM		data reduction
SCALA		data scaling

Refinement

Method to determine structure:

SAD / Resolution: 3.4→29.915 Å / SU ML: 0.51 / Isotropic thermal model: isotropic / σ(F): 0.91 / Phase error: 33.79 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.3127	5051	4.98 %
R_work	0.265	-	-
obs	0.2673	101419	98.74 %
all	-	102713	-

Solvent computation

Shrinkage radii: 1 Å / VDW probe radii: 1.4 Å / Solvent model: FLAT BULK SOLVENT MODEL / B_sol: 80 Å² / k_sol: 0.2 e/Å³

Displacement parameters

	B_aniso -1	B_aniso -2	B_aniso -3
1-	1.4017 Å²	0 Å²	7.917 Å²
2-	-	6.68 Å²	-0 Å²
3-	-	-	-8.0817 Å²

Refinement step

Cycle: LAST / Resolution: 3.4→29.915 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	23034	0	162	0	23196

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.01	23526
X-RAY DIFFRACTION	f_angle_d	1.298	31746
X-RAY DIFFRACTION	f_dihedral_angle_d	17.474	9030
X-RAY DIFFRACTION	f_chiral_restr	0.073	3714
X-RAY DIFFRACTION	f_plane_restr	0.004	4032

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Number	Refine-ID	Type	Rms dev position (Å)
1	1	A	419	X-RAY DIFFRACTION	POSITIONAL
1	2	B	419	X-RAY DIFFRACTION	POSITIONAL	0.018
1	3	C	419	X-RAY DIFFRACTION	POSITIONAL	0.023
1	4	D	419	X-RAY DIFFRACTION	POSITIONAL	0.017
1	5	E	419	X-RAY DIFFRACTION	POSITIONAL	0.018
1	6	F	419	X-RAY DIFFRACTION	POSITIONAL	0.015
2	1	A	1191	X-RAY DIFFRACTION	POSITIONAL
2	2	B	1191	X-RAY DIFFRACTION	POSITIONAL	0.047
2	3	C	1191	X-RAY DIFFRACTION	POSITIONAL	0.048
2	4	D	1191	X-RAY DIFFRACTION	POSITIONAL	0.062
2	5	E	1191	X-RAY DIFFRACTION	POSITIONAL	0.052
2	6	F	1191	X-RAY DIFFRACTION	POSITIONAL	0.055
3	1	A	755	X-RAY DIFFRACTION	POSITIONAL
3	2	B	755	X-RAY DIFFRACTION	POSITIONAL	0.067
3	3	C	755	X-RAY DIFFRACTION	POSITIONAL	0.062
3	4	D	755	X-RAY DIFFRACTION	POSITIONAL	0.068
3	5	E	755	X-RAY DIFFRACTION	POSITIONAL	0.066
3	6	F	755	X-RAY DIFFRACTION	POSITIONAL	0.065
4	1	A	1332	X-RAY DIFFRACTION	POSITIONAL
4	2	B	1332	X-RAY DIFFRACTION	POSITIONAL	0.055
4	3	C	1332	X-RAY DIFFRACTION	POSITIONAL	0.048
4	4	D	1332	X-RAY DIFFRACTION	POSITIONAL	0.055
4	5	E	1332	X-RAY DIFFRACTION	POSITIONAL	0.052
4	6	F	1332	X-RAY DIFFRACTION	POSITIONAL	0.054

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.4-3.4386	0.4549	200	0.3821	3329	X-RAY DIFFRACTION	99
3.4386-3.479	0.3992	202	0.3646	3073	X-RAY DIFFRACTION	99
3.479-3.5213	0.4137	153	0.3623	3229	X-RAY DIFFRACTION	99
3.5213-3.5658	0.4195	169	0.3528	3322	X-RAY DIFFRACTION	99
3.5658-3.6127	0.4138	183	0.3533	3108	X-RAY DIFFRACTION	99
3.6127-3.6621	0.3898	174	0.3406	3265	X-RAY DIFFRACTION	99
3.6621-3.7143	0.3454	153	0.3003	3243	X-RAY DIFFRACTION	99
3.7143-3.7697	0.3723	172	0.2815	3194	X-RAY DIFFRACTION	99
3.7697-3.8284	0.3254	187	0.2768	3293	X-RAY DIFFRACTION	99
3.8284-3.8911	0.3275	146	0.2872	3182	X-RAY DIFFRACTION	99
3.8911-3.958	0.3821	174	0.2825	3240	X-RAY DIFFRACTION	99
3.958-4.0298	0.3002	128	0.2816	3278	X-RAY DIFFRACTION	100
4.0298-4.1072	0.4058	161	0.2717	3329	X-RAY DIFFRACTION	100
4.1072-4.1908	0.2623	158	0.2519	3195	X-RAY DIFFRACTION	100
4.1908-4.2816	0.3356	211	0.2524	3185	X-RAY DIFFRACTION	100
4.2816-4.3809	0.2717	198	0.2341	3194	X-RAY DIFFRACTION	100
4.3809-4.4902	0.2882	146	0.2274	3307	X-RAY DIFFRACTION	100
4.4902-4.6111	0.2861	172	0.2253	3210	X-RAY DIFFRACTION	100
4.6111-4.7463	0.2994	174	0.2354	3274	X-RAY DIFFRACTION	100
4.7463-4.8989	0.2931	170	0.2336	3268	X-RAY DIFFRACTION	100
4.8989-5.0731	0.306	140	0.2247	3244	X-RAY DIFFRACTION	100
5.0731-5.2752	0.304	171	0.2376	3231	X-RAY DIFFRACTION	100
5.2752-5.5139	0.3338	175	0.2598	3270	X-RAY DIFFRACTION	100
5.5139-5.8026	0.3492	170	0.2665	3221	X-RAY DIFFRACTION	99
5.8026-6.1632	0.3334	172	0.2586	3207	X-RAY DIFFRACTION	100
6.1632-6.6343	0.2564	149	0.2456	3236	X-RAY DIFFRACTION	99
6.6343-7.2931	0.2595	168	0.2187	3202	X-RAY DIFFRACTION	99
7.2931-8.3285	0.2444	171	0.2043	3041	X-RAY DIFFRACTION	93
8.3285-10.4188	0.1989	189	0.1748	3129	X-RAY DIFFRACTION	97
10.4188-29.9161	0.2903	115	0.2806	2869	X-RAY DIFFRACTION	87

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.4337	-0.8042	1.1217	0.8947	-0.6847	1.0105	-0.3652	0.3841	-0.3612	0.2945	1.201	0.6748	-0.5886	-0.3812	0.0467	1.0648	-0.7465	-0.0956	-0.0013	-0.0639	2.1979	72.4859	20.6387	10.8574
2	0.2004	-0.0664	0.0939	0.1453	0.0904	0.2472	0.4526	-0.7108	-0.2421	-0.0291	0.9757	-0.8724	-0.0709	0.6513	0.0342	0.6228	0.0618	-0.3768	1.2409	0.6257	1.8847	51.3406	27.7171	62.9904
3	0.0372	-0.0354	-0.0268	-0.0052	0.0076	0.0107	-0.3541	-0.4732	-0.6039	-0.2634	-0.9883	0.3293	0.9763	0.4469	-0.0088	2.5589	0.5054	-1.884	2.2113	-1.4562	3.2379	67.5121	39.3758	22.3854
4	0.085	-0.0491	-0.0509	-0.0359	0.0678	0.0572	-0.0821	-0.1556	0.5289	0.6016	-0.2198	-1.1826	-0.4738	-0.5715	-0.0029	1.1565	-0.5228	0.21	2.4323	0.221	1.852	80.0296	-1.8761	27.5791
5	0.0488	0.0514	0.0099	0.0592	0.0557	0.1489	-0.3381	-1.0182	0.9935	0.1511	-0.7597	-0.1955	0.4636	0.4009	-0.0178	1.6394	0.2922	-0.5673	2.097	0.6409	1.9275	59.3311	40.8057	45.7838
6	0.09	-0.1409	0.0057	0.2871	-0.3236	0.5284	0.406	-0.1883	-0.4855	0.6408	1.3525	0.6839	1.1833	-0.8506	0.0401	1.8469	0.2311	-0.4086	1.3383	-0.8976	1.9893	34.944	9.5017	59.2042
7	0.9203	-0.4459	0.3008	0.9632	-1.0419	3.743	0.2888	0.636	-0.4678	0.7214	-0.1914	-0.1804	0.1572	-0.1455	1.1683	0.6004	0.0292	-0.1897	0.1861	-0.3123	0.7093	41.0533	18.0865	1.5604
8	4.3337	0.5684	-1.3875	3.0043	0.7138	1.9891	0.5199	0.0794	0.9951	-0.4347	-0.2923	-0.1202	-0.4143	0.2834	-1.4915	0.6257	-0.3547	-0.0147	0.2953	0.1012	0.3666	25.093	44.4903	74.1573
9	1.8788	0.6442	1.3344	1.6301	-0.5591	8.0154	1.5285	1.0653	-0.4896	1.1644	0.2395	0.6859	0.9704	-0.2152	2.7224	0.729	0.1924	-0.0399	0.6681	-0.274	-0.1763	37.6654	50.5554	12.5794
10	0.9294	0.1921	0.3522	1.8641	0.8556	1.1331	0.4031	0.5401	-0.4784	-0.6629	0.1429	0.0177	-1.6253	0.2753	1.2165	1.0815	-0.2446	-0.0809	-0.067	0.032	0.3619	48.4842	-10.6882	21.0301
11	0.4232	-0.1715	-0.6003	1.6263	0.3679	0.6035	1.049	0.568	0.2049	-0.1616	-1.2575	0.7319	0.2098	-0.0062	-0.0981	1.1904	-0.0936	0.3134	0.1436	0.6521	0.2342	33.535	60.7878	44.6116
12	0.5691	-0.2915	-0.3645	1.1475	0.0843	0.7255	-0.0816	-0.2372	-0.4764	0.0475	0.0392	0.3093	0.0391	-0.3112	0.1412	0.744	-0.7318	0.1496	1.3616	-0.6534	0.7826	10.1286	12.6407	80.1744
13	1.996	0.5634	-0.6096	3.208	-2.2892	2.196	0.6964	-0.5855	0.953	0.992	2.2177	3.4918	-0.2971	-1.0936	4.6878	0.2635	-0.6419	-1.2388	-0.3949	-1.1333	-1.2006	29.5518	-12.0773	2.6677
14	0.5834	-0.096	-0.3313	0.2927	-1.2541	2.876	0.3342	0.8085	-0.5449	0.5464	0.2578	0.1113	-2.1268	-1.0273	-0.0757	1.5365	-0.3288	0.2752	0.036	0.1339	0.5731	21.2186	74.6007	62.4534
15	1.9259	-1.8423	-0.3762	3.444	1.6173	1.9346	-0.096	-0.0725	-0.8707	-0.5793	-0.5535	2.2112	0.3548	-0.1001	-0.4737	0.5088	-0.0136	-0.3633	0.7676	0.1744	0.085	24.3514	33.2839	-11.3844
16	1.111	-0.5403	1.3448	1.2961	-0.6213	3.0279	-0.3894	-1.5422	0.0658	0.287	0.5969	-0.0429	0.4599	-2.4195	-0.2854	0.5604	0.2426	0.156	-0.1115	0.5298	0.4197	39.3426	-28.3871	45.5552
17	0.7978	0.272	1.0893	0.6348	1.0959	4.8283	0.0684	-0.6521	-0.2459	-0.441	-0.5431	-0.3506	-0.4713	-2.2207	-0.3057	0.8832	0.2792	0.1966	0.2291	0.4461	0.5341	24.4632	72.2306	15.5734
18	2.1398	0.8659	0.5267	0.8129	0.1888	0.4269	0.2141	1.7737	0.1909	0.6153	0.5385	0.9485	-0.4623	0.0059	0.0825	0.5745	-0.2495	0.0819	0.5942	0.0763	0.1171	8.6563	41.6321	94.5557
19	2.0666	0.5092	-0.6836	3.7587	1.1381	1.7993	0.154	0.8333	0.3686	0.0228	0.7224	0.0244	0.0324	-0.5651	0.8104	0.1896	0.2217	-0.2659	0.738	0.1632	0.7093	3.5028	12.6674	14.1358
20	1.1242	0.5984	-0.2196	1.7871	0.3129	1.0364	0.3971	-0.7271	-0.1044	0.4253	-0.4538	-0.5489	0.6171	-1.0875	0.0379	0.487	-0.0524	0.1671	1.3388	0.2197	0.5229	-11.58	56.8678	65.4298
21	0.675	0.1652	-0.0828	1.7343	0.419	2.3891	0.1851	0.0138	0.2496	0.2933	-0.0469	0.1162	0.0455	0.6874	0.0297	0.2527	0.2895	0.0708	0.6944	0.2663	0.5463	-1.4869	42.5343	13.7403
22	1.4668	-0.4959	0.9276	1.1945	-0.6098	1.1257	-1.0768	-1.0735	-0.1832	0.843	0.5821	-0.0805	-1.3688	-0.4152	-0.1328	0.6008	0.6178	-0.0128	1.1189	0.1451	0.4356	11.9933	-4.9443	36.792
23	0.2731	0.1682	0.3152	1.321	0.4957	0.684	-0.3462	-0.5045	-0.3153	-0.5356	-0.2085	-0.2592	0.3052	0.0052	-0.0098	0.4918	0.45	0.2912	0.8472	0.2352	0.549	-5.2223	61.707	36.4908
24	1.1083	-0.7068	0.6512	1.1672	0.5784	0.729	-1.0098	0.7342	-0.8789	0.2878	0.6145	0.2105	-0.722	0.5195	-0.0924	0.2361	-0.4833	0.1807	1.4142	0.0954	0.7405	-24.2639	33.2853	79.883

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain A AND resseq 246:298
2	X-RAY DIFFRACTION	2	chain B AND resseq 246:298
3	X-RAY DIFFRACTION	3	chain C AND resseq 246:298
4	X-RAY DIFFRACTION	4	chain D AND resseq 246:298
5	X-RAY DIFFRACTION	5	chain E AND resseq 246:298
6	X-RAY DIFFRACTION	6	chain F AND resseq 246:298
7	X-RAY DIFFRACTION	7	chain A AND resseq 308:490
8	X-RAY DIFFRACTION	8	chain B AND resseq 308:490
9	X-RAY DIFFRACTION	9	chain C AND resseq 308:490
10	X-RAY DIFFRACTION	10	chain D AND resseq 308:490
11	X-RAY DIFFRACTION	11	chain E AND resseq 308:490
12	X-RAY DIFFRACTION	12	chain F AND resseq 308:490
13	X-RAY DIFFRACTION	13	chain A AND resseq 491:582
14	X-RAY DIFFRACTION	14	chain B AND resseq 491:582
15	X-RAY DIFFRACTION	15	chain C AND resseq 491:582
16	X-RAY DIFFRACTION	16	chain D AND resseq 491:582
17	X-RAY DIFFRACTION	17	chain E AND resseq 491:582
18	X-RAY DIFFRACTION	18	chain F AND resseq 491:582
19	X-RAY DIFFRACTION	19	chain A AND resseq 591:770
20	X-RAY DIFFRACTION	20	chain B AND resseq 591:770
21	X-RAY DIFFRACTION	21	chain C AND resseq 591:770
22	X-RAY DIFFRACTION	22	chain D AND resseq 591:770
23	X-RAY DIFFRACTION	23	chain E AND resseq 591:770
24	X-RAY DIFFRACTION	24	chain F AND resseq 591:770

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