Protein / Protein/peptide , 2 types, 6 molecules ABCDPQ
#1: Protein
Neurolysin, mitochondrial / / Angiotensin-binding protein / Microsomal endopeptidase / MEP / Mitochondrial oligopeptidase M / ...Angiotensin-binding protein / Microsomal endopeptidase / MEP / Mitochondrial oligopeptidase M / Neurotensin endopeptidase
Mass: 78644.781 Da / Num. of mol.: 2 / Mutation: E475Q Source method: isolated from a genetically manipulated source Details: Catalytically impaired Human neurolysin / Source: (gene. exp.) Homo sapiens (human) / Gene: NLN, AGTBP, KIAA1226 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta 2 / References: UniProt: Q9BYT8, neurolysin
#2: Protein/peptide
PRO-ARG-ARG-PROneurotensinfragment
Mass: 1675.948 Da / Num. of mol.: 4 / Source method: obtained synthetically Details: Neurotensin product of the cleavage reaction by human neurolysin Source: (synth.) Homo sapiens (human) / References: UniProt: P30990*PLUS
Resolution: 2.7→50 Å / Cor.coef. Fo:Fc: 0.928 / Cor.coef. Fo:Fc free: 0.895 / SU B: 32.777 / SU ML: 0.305 / Cross valid method: THROUGHOUT / ESU R: 2.661 / ESU R Free: 0.368 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.26786
2301
4.9 %
RANDOM
Rwork
0.21558
-
-
-
obs
0.21813
44361
98.07 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å