+Open data
-Basic information
Entry | Database: PDB / ID: 1i1i | ||||||
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Title | NEUROLYSIN (ENDOPEPTIDASE 24.16) CRYSTAL STRUCTURE | ||||||
Components | NEUROLYSIN | ||||||
Keywords | HYDROLASE / neuropeptidase / zinc metallopeptidase / endopeptidase | ||||||
Function / homology | Function and homology information neurolysin / regulation of skeletal muscle fiber differentiation / Peptide ligand-binding receptors / oligopeptidase activity / peptide metabolic process / regulation of gluconeogenesis / peptide catabolic process / peptide binding / protein catabolic process / mitochondrial intermembrane space ...neurolysin / regulation of skeletal muscle fiber differentiation / Peptide ligand-binding receptors / oligopeptidase activity / peptide metabolic process / regulation of gluconeogenesis / peptide catabolic process / peptide binding / protein catabolic process / mitochondrial intermembrane space / metalloendopeptidase activity / peptidase activity / proteolysis / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 2.3 Å | ||||||
Authors | Brown, C.K. / Madauss, K. / Lian, W. / Tolbert, W.D. / Beck, M.R. / Rodgers, D.W. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2001 Title: Structure of neurolysin reveals a deep channel that limits substrate access. Authors: Brown, C.K. / Madauss, K. / Lian, W. / Beck, M.R. / Tolbert, W.D. / Rodgers, D.W. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Crystallization and Preliminary Analysis of Neurolysin Authors: Lian, W. / Chen, G. / Wu, D. / Brown, C.K. / Madauss, K. / Hersh, L.B. / Rodgers, D.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1i1i.cif.gz | 146 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1i1i.ent.gz | 113.8 KB | Display | PDB format |
PDBx/mmJSON format | 1i1i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1i1i_validation.pdf.gz | 431.6 KB | Display | wwPDB validaton report |
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Full document | 1i1i_full_validation.pdf.gz | 445.1 KB | Display | |
Data in XML | 1i1i_validation.xml.gz | 27.3 KB | Display | |
Data in CIF | 1i1i_validation.cif.gz | 37.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i1/1i1i ftp://data.pdbj.org/pub/pdb/validation_reports/i1/1i1i | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 77874.859 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Plasmid: PBADC / Production host: Escherichia coli (E. coli) / Strain (production host): TOP10 / References: UniProt: P42676, neurolysin | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 51 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 8000, cacodylate, magnesium chloride, zinc chloride, 2-mercaptoethanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystal grow | *PLUS Method: unknown |
Components of the solutions | *PLUS Conc.: 0.2 mM / Chemical formula: MeHgCl |
-Data collection
Diffraction | Mean temperature: 108 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-BM-C / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 12, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. obs: 36511 / % possible obs: 99 % / Observed criterion σ(I): -3 / Redundancy: 3.9 % / Rmerge(I) obs: 0.042 / Net I/σ(I): 23.9 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.156 / Mean I/σ(I) obs: 7.8 / % possible all: 95.5 |
Reflection shell | *PLUS % possible obs: 95.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS / Resolution: 2.3→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||
Refine LS restraints | *PLUS
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