温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: The HODM-heme subunit was crystallised at 3 mg ml-1 in 50 mM KPi, 100 mM KCl, pH 7.5. Initial crystallisation conditions were identified using the JCSG+ matrix screen (Molecular dimensions). ...詳細: The HODM-heme subunit was crystallised at 3 mg ml-1 in 50 mM KPi, 100 mM KCl, pH 7.5. Initial crystallisation conditions were identified using the JCSG+ matrix screen (Molecular dimensions). Crystals suitable for diffraction experiments were obtained by sitting drop vapour diffusion at 277 K in 400 nL drops containing equal volumes of protein and a solution containing 30% PEG 2K MME and 0.1 M potassium thiocyanate
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.917 Å / 相対比: 1
反射
解像度: 1.65→26.82 Å / Num. obs: 53715 / % possible obs: 99.93 % / 冗長度: 12.1 % / Rmerge(I) obs: 0.061 / Net I/σ(I): 15.4
反射 シェル
解像度: 1.65→1.7 Å / 冗長度: 12.2 % / Rmerge(I) obs: 0.783 / Mean I/σ(I) obs: 3.8 / % possible all: 100
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.7.0029
精密化
XDS
データ削減
XDS
データスケーリング
MLPHARE
位相決定
精密化
構造決定の手法: 多重同系置換 / 解像度: 1.65→26.82 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.948 / SU B: 1.333 / SU ML: 0.046 / 交差検証法: THROUGHOUT / ESU R: 0.076 / ESU R Free: 0.079 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS