+Open data
-Basic information
Entry | Database: PDB / ID: 5lmy | |||||||||
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Title | Solution structure of the m-pmv myristoylated matrix protein | |||||||||
Components | Matrix protein p10 | |||||||||
Keywords | VIRAL PROTEIN / Matrix / M-PMV / Myristoylated / Retrovirus | |||||||||
Function / homology | Function and homology information viral budding via host ESCRT complex / viral nucleocapsid / host cell cytoplasm / nucleic acid binding / structural constituent of virion / zinc ion binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Mason-Pfizer monkey virus | |||||||||
Method | SOLUTION NMR | |||||||||
Authors | Prchal, J. / Hrabal, R. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 2016 Title: Membrane Interactions of the Mason-Pfizer Monkey Virus Matrix Protein and Its Budding Deficient Mutants. Authors: Kroupa, T. / Langerova, H. / Dolezal, M. / Prchal, J. / Spiwok, V. / Hunter, E. / Rumlova, M. / Hrabal, R. / Ruml, T. #1: Journal: J. Mol. Biol. / Year: 2012 Title: The structure of myristoylated Mason-Pfizer monkey virus matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in its membrane binding. Authors: Prchal, J. / Srb, P. / Hunter, E. / Ruml, T. / Hrabal, R. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5lmy.cif.gz | 610.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5lmy.ent.gz | 532.9 KB | Display | PDB format |
PDBx/mmJSON format | 5lmy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lm/5lmy ftp://data.pdbj.org/pub/pdb/validation_reports/lm/5lmy | HTTPS FTP |
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-Related structure data
Related structure data | 5ldlC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14923.988 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Myristoylated Matrix protein of Mason-Pfizer Monkey Virus. N-terminaly myristoylated with C-terminal extension with 20 AA from phospohoprotein (pp24) and his-tag. Source: (gene. exp.) Mason-Pfizer monkey virus / Gene: gag / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P07567 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Bruker AvanceIII / Manufacturer: Bruker / Model: AvanceIII / Field strength: 600 MHz |
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-Processing
Software | Name: X-PLOR / Version: NIH 2.24 / Classification: refinement | ||||||||||||||||||||
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NMR software |
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NMR ensemble | Conformers submitted total number: 15 |