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Yorodumi- PDB-5l23: Crystal structure of the complex between the N-terminal SH3 domai... -
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-Basic information
Entry | Database: PDB / ID: 5l23 | ||||||
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Title | Crystal structure of the complex between the N-terminal SH3 domain of CrkII and a proline-rich ligand | ||||||
Components |
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Keywords | PROTEIN BINDING / SH3 / acetylation / amidation / Crkii / C3G | ||||||
Function / homology | Function and homology information positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis / : / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / ARMS-mediated activation / MET activates RAP1 and RAC1 / MET receptor recycling / response to hepatocyte growth factor / cellular response to endothelin / helper T cell diapedesis / cerebellar neuron development ...positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis / : / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / ARMS-mediated activation / MET activates RAP1 and RAC1 / MET receptor recycling / response to hepatocyte growth factor / cellular response to endothelin / helper T cell diapedesis / cerebellar neuron development / response to cholecystokinin / regulation of leukocyte migration / Downstream signal transduction / response to peptide / postsynaptic specialization assembly / Rap protein signal transduction / protein phosphorylated amino acid binding / regulation of T cell migration / negative regulation of neural precursor cell proliferation / p130Cas linkage to MAPK signaling for integrins / reelin-mediated signaling pathway / regulation of dendrite development / regulation of cell junction assembly / positive regulation of skeletal muscle acetylcholine-gated channel clustering / negative regulation of natural killer cell mediated cytotoxicity / response to yeast / Regulation of signaling by CBL / Regulation of actin dynamics for phagocytic cup formation / regulation of Rac protein signal transduction / nerve growth factor signaling pathway / negative regulation of wound healing / negative regulation of cell motility / protein localization to membrane / VEGFA-VEGFR2 Pathway / negative regulation of Ras protein signal transduction / establishment of endothelial barrier / positive regulation of Ras protein signal transduction / activation of GTPase activity / MET activates RAP1 and RAC1 / cellular response to insulin-like growth factor stimulus / establishment of cell polarity / Frs2-mediated activation / regulation of GTPase activity / enzyme-linked receptor protein signaling pathway / blood vessel development / positive regulation of smooth muscle cell migration / dendrite development / positive regulation of Rac protein signal transduction / regulation of cell adhesion mediated by integrin / regulation of JNK cascade / platelet-derived growth factor receptor signaling pathway / cellular response to nitric oxide / ephrin receptor signaling pathway / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / signaling adaptor activity / Erythropoietin activates RAS / positive regulation of substrate adhesion-dependent cell spreading / cellular response to cAMP / cellular response to transforming growth factor beta stimulus / cell surface receptor protein tyrosine kinase signaling pathway / cytoskeletal protein binding / ephrin receptor binding / phosphotyrosine residue binding / Downstream signal transduction / SH2 domain binding / guanyl-nucleotide exchange factor activity / protein tyrosine kinase binding / cell chemotaxis / cellular response to nerve growth factor stimulus / positive regulation of GTPase activity / Regulation of signaling by CBL / hippocampus development / regulation of actin cytoskeleton organization / positive regulation of JNK cascade / insulin-like growth factor receptor binding / neuron migration / negative regulation of canonical Wnt signaling pathway / response to hydrogen peroxide / neuromuscular junction / lipid metabolic process / negative regulation of ERK1 and ERK2 cascade / receptor tyrosine kinase binding / cerebral cortex development / cytokine-mediated signaling pathway / positive regulation of neuron projection development / cell-cell adhesion / SH3 domain binding / phagocytic vesicle membrane / : / signaling receptor complex adaptor activity / cell migration / actin cytoskeleton / protein-macromolecule adaptor activity / nervous system development / regulation of cell shape / actin cytoskeleton organization / scaffold protein binding / cell population proliferation / positive regulation of ERK1 and ERK2 cascade / early endosome Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.77 Å | ||||||
Authors | Bhatt, V.S. / Krieger, I. / Sacchettini, J. / Cho, J.-H. | ||||||
Citation | Journal: To Be Published Title: Crystal structure of the complex between the N-terminal SH3 domain of CrkII and a proline-rich ligand Authors: Bhatt, V.S. / Krieger, I. / Sacchettini, J. / Cho, J.-H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5l23.cif.gz | 32 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5l23.ent.gz | 20.4 KB | Display | PDB format |
PDBx/mmJSON format | 5l23.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5l23_validation.pdf.gz | 438 KB | Display | wwPDB validaton report |
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Full document | 5l23_full_validation.pdf.gz | 440 KB | Display | |
Data in XML | 5l23_validation.xml.gz | 7.3 KB | Display | |
Data in CIF | 5l23_validation.cif.gz | 9.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l2/5l23 ftp://data.pdbj.org/pub/pdb/validation_reports/l2/5l23 | HTTPS FTP |
-Related structure data
Related structure data | 1ckaS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 6971.731 Da / Num. of mol.: 1 / Fragment: UNP residues 134-191 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Crk, Crko / Production host: Escherichia coli (E. coli) / References: UniProt: Q64010 | ||
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#2: Protein/peptide | Mass: 1724.958 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Mus musculus (house mouse) / References: UniProt: Q13905*PLUS | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.86 Å3/Da / Density % sol: 34 % |
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Crystal grow | Temperature: 286 K / Method: vapor diffusion, sitting drop / Details: Lithium sulfate monohydrate, PEG 3350 |
-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.54 Å |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: May 1, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.77→33.43 Å / Num. obs: 6759 / % possible obs: 100 % / Redundancy: 7.4 % / CC1/2: 0.977 / Rmerge(I) obs: 0.16 / Net I/σ(I): 11.7 |
Reflection shell | Resolution: 1.77→1.87 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.2 / Mean I/σ(I) obs: 8.4 / CC1/2: 0.96 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1cka Resolution: 1.77→33.43 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.942 / Cross valid method: THROUGHOUT / ESU R: 0.146 / ESU R Free: 0.129 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.616 Å2
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Refinement step | Cycle: LAST / Resolution: 1.77→33.43 Å
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