- PDB-5kua: Cryo-EM reconstruction of Neisseria meningitidis Type IV pilus -
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Open data
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Basic information
Entry
Database: PDB / ID: 5kua
Title
Cryo-EM reconstruction of Neisseria meningitidis Type IV pilus
Components
pilin
Keywords
PROTEIN FIBRIL / melted helix / Type IV pili
Function / homology
Function and homology information
protein secretion by the type II secretion system / type II protein secretion system complex / pilus / cell adhesion / membrane Similarity search - Function
Journal: Nat Commun / Year: 2016 Title: Structure of the Neisseria meningitidis Type IV pilus. Authors: Subramania Kolappan / Mathieu Coureuil / Xiong Yu / Xavier Nassif / Edward H Egelman / Lisa Craig / Abstract: Neisseria meningitidis use Type IV pili (T4P) to adhere to endothelial cells and breach the blood brain barrier, causing cause fatal meningitis. T4P are multifunctional polymers of the major pilin ...Neisseria meningitidis use Type IV pili (T4P) to adhere to endothelial cells and breach the blood brain barrier, causing cause fatal meningitis. T4P are multifunctional polymers of the major pilin protein, which share a conserved hydrophobic N terminus that is a curved extended α-helix, α1, in X-ray crystal structures. Here we report a 1.44 Å crystal structure of the N. meningitidis major pilin PilE and a ∼6 Å cryo-electron microscopy reconstruction of the intact pilus, from which we built an atomic model for the filament. This structure reveals the molecular arrangement of the N-terminal α-helices in the filament core, including a melted central portion of α1 and a bridge of electron density consistent with a predicted salt bridge necessary for pilus assembly. This structure has important implications for understanding pilus biology.
Helical symmetry: (Circular symmetry: 1 / Dyad axis: no / N subunits divisor: 1 / Num. of operations: 26 / Rise per n subunits: 10.3 Å / Rotation per n subunits: 100.8 °)
Details
The biological unit is a helical fibril comprising an indeterminate number of identical subunits. The provided helical parameters should be applied to a single subunit to generate an extended fibril.
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Components
#1: Protein
... pilin
Mass: 17067.182 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Neisseria meningitidis (bacteria) / References: UniProt: A0A1I9GEU1*PLUS
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Experimental details
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Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction
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Sample preparation
Component
Name: Type IV pilus / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: NATURAL
Source (natural)
Organism: Neisseria meningitidis (bacteria)
Buffer solution
pH: 10.5
Specimen
Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Vitrification
Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 90 % / Chamber temperature: 297 K / Details: Plunged into liquid ethane (FEI VITROBOT MARK IV)
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Electron microscopy imaging
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
Microscopy
Model: FEI TITAN KRIOS
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lens
Mode: BRIGHT FIELD
Specimen holder
Cryogen: NITROGEN
Image recording
Electron dose: 40 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)
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