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- PDB-5k36: Structure of an eleven component nuclear RNA exosome complex boun... -

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Basic information

Entry
Database: PDB / ID: 5k36
TitleStructure of an eleven component nuclear RNA exosome complex bound to RNA
Components
  • (Exosome complex component ...) x 9
  • (Exosome complex exonuclease ...) x 2
  • RNA (17-MER)
KeywordsHydrolase/RNA / Exoribonuclease / Complex / RNA / STRUCTURAL PROTEIN / Hydrolase-RNA complex
Function / homology
Function and homology information


nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent antisense transcript catabolic process / mRNA decay by 3' to 5' exoribonuclease / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / nuclear polyadenylation-dependent mRNA catabolic process / nuclear mRNA surveillance of mRNA 3'-end processing / regulatory ncRNA 3'-end processing / U1 snRNA 3'-end processing ...nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent antisense transcript catabolic process / mRNA decay by 3' to 5' exoribonuclease / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / nuclear polyadenylation-dependent mRNA catabolic process / nuclear mRNA surveillance of mRNA 3'-end processing / regulatory ncRNA 3'-end processing / U1 snRNA 3'-end processing / nuclear polyadenylation-dependent CUT catabolic process / U5 snRNA 3'-end processing / TRAMP-dependent tRNA surveillance pathway / CUT catabolic process / exosome (RNase complex) / U4 snRNA 3'-end processing / cytoplasmic exosome (RNase complex) / nuclear polyadenylation-dependent rRNA catabolic process / poly(A)-dependent snoRNA 3'-end processing / nuclear exosome (RNase complex) / nuclear-transcribed mRNA catabolic process, non-stop decay / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / histone mRNA catabolic process / nuclear mRNA surveillance / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / rRNA catabolic process / mRNA 3'-UTR AU-rich region binding / rRNA primary transcript binding / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / RNA catabolic process / regulation of telomere maintenance / nonfunctional rRNA decay / rRNA metabolic process / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA catabolic process / nuclear-transcribed mRNA catabolic process / RNA processing / RNA endonuclease activity / mRNA processing / manganese ion binding / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / 3'-5'-RNA exonuclease activity / endonuclease activity / tRNA binding / single-stranded RNA binding / nucleotide binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / nucleus / cytoplasm
Similarity search - Function
OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #700 / : / Exosome complex component CSL4, N-terminal domain / Exosome complex exonuclease Rrp40, N-terminal / Exosome complex exonuclease Rrp40 N-terminal domain / Exosome-associated factor Rrp6, N-terminal / Exosome complex exonuclease Rrp6-like / : / PMC2NT (NUC016) domain / Exosome complex exonuclease RRP44, S1 domain ...OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #700 / : / Exosome complex component CSL4, N-terminal domain / Exosome complex exonuclease Rrp40, N-terminal / Exosome complex exonuclease Rrp40 N-terminal domain / Exosome-associated factor Rrp6, N-terminal / Exosome complex exonuclease Rrp6-like / : / PMC2NT (NUC016) domain / Exosome complex exonuclease RRP44, S1 domain / S1 domain / PIN domain / HRDC domain / Exosome complex component RRP45 / Rrp40, S1 domain / : / Exosome complex component RRP40, S1 domain / Exosome complex component CSL4, C-terminal / Exosome complex component, N-terminal domain / Exosome complex component Csl4 / Exosome component EXOSC1/CSL4 / Exosome complex exonuclease RRP4 N-terminal region / RRP4, S1 domain / Rrp44-like cold shock domain / Rrp44-like cold shock domain / : / KH domain / Exosome complex RNA-binding protein 1/RRP40/RRP4 / Dis3-like cold-shock domain 2 / Dis3-like cold-shock domain 2 (CSD2) / GHMP Kinase, N-terminal domain / : / Ribonuclease II/R, conserved site / Ribonuclease II family signature. / : / Ribonuclease II/R / RNB domain / RNB / : / Helicase and RNase D C-terminal / HRDC domain / HRDC domain / HRDC domain profile. / K Homology domain, type 1 / Exoribonuclease, phosphorolytic domain 2 / 3' exoribonuclease family, domain 2 / Exoribonuclease, phosphorolytic domain 1 / PNPase/RNase PH domain superfamily / Exoribonuclease, PH domain 2 superfamily / 3' exoribonuclease family, domain 1 / HRDC domain superfamily / Large family of predicted nucleotide-binding domains / 3'-5' exonuclease / PIN domain / K Homology domain, type 1 / 3'-5' exonuclease / 3'-5' exonuclease domain / Ribosomal Protein S8; Chain: A, domain 1 / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / K Homology domain, type 1 superfamily / PIN-like domain superfamily / Ribosomal Protein S5; domain 2 / S1 domain profile. / HRDC-like superfamily / Ribosomal protein S1-like RNA-binding domain / S1 domain / Nucleic acid-binding proteins / Ribonuclease H-like superfamily/Ribonuclease H / DNA polymerase; domain 1 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Nucleotidyltransferase; domain 5 / Ribonuclease H superfamily / Ribonuclease H-like superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold / Beta Barrel / 2-Layer Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
RNA / RNA (> 10) / Exosome complex component RRP43 / Exosome complex component RRP4 / Exosome complex component SKI6 / Exosome complex component MTR3 / Exosome complex component RRP46 / Exosome complex component CSL4 / Exosome complex component RRP45 / Exosome complex exonuclease DIS3 ...RNA / RNA (> 10) / Exosome complex component RRP43 / Exosome complex component RRP4 / Exosome complex component SKI6 / Exosome complex component MTR3 / Exosome complex component RRP46 / Exosome complex component CSL4 / Exosome complex component RRP45 / Exosome complex exonuclease DIS3 / Exosome complex component RRP40 / Exosome complex exonuclease RRP6 / Exosome complex component RRP42
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.1 Å
AuthorsLima, C.D. / Zinder, J.C.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM079196 United States
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Mol.Cell / Year: 2016
Title: Nuclear RNA Exosome at 3.1 angstrom Reveals Substrate Specificities, RNA Paths, and Allosteric Inhibition of Rrp44/Dis3.
Authors: Zinder, J.C. / Wasmuth, E.V. / Lima, C.D.
History
DepositionMay 19, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 2, 2016Provider: repository / Type: Initial release
Revision 1.1Dec 7, 2016Group: Database references
Revision 1.2Sep 20, 2017Group: Author supporting evidence / Database references / Category: citation / pdbx_audit_support
Item: _citation.journal_id_CSD / _pdbx_audit_support.funding_organization
Revision 1.3Nov 1, 2017Group: Author supporting evidence / Category: pdbx_struct_assembly_auth_evidence
Revision 1.4Nov 20, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.5Sep 27, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Exosome complex component RRP45
B: Exosome complex component SKI6
C: Exosome complex component RRP43
D: Exosome complex component RRP46
E: Exosome complex component RRP42
F: Exosome complex component MTR3
G: Exosome complex component RRP40
H: Exosome complex component RRP4
I: Exosome complex component CSL4
J: Exosome complex exonuclease RRP6
K: Exosome complex exonuclease DIS3
M: RNA (17-MER)
L: RNA (17-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)477,52031
Polymers475,85013
Non-polymers1,67118
Water5,044280
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration, assay for oligomerization
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area62510 Å2
ΔGint-444 kcal/mol
Surface area152450 Å2
Unit cell
Length a, b, c (Å)140.701, 212.257, 218.231
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
DetailsAsymmetric unit is the biological assembly according to gel filtration chromatography and activity assays

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Components

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Exosome complex component ... , 9 types, 9 molecules ABCDEFGHI

#1: Protein Exosome complex component RRP45 / Ribosomal RNA-processing protein 45


Mass: 34001.859 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: RRP45, YDR280W, D9954.1 / Plasmid: pRSFduet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: Q05636
#2: Protein Exosome complex component SKI6 / Extracellular mutant protein 20 / Ribosomal RNA-processing protein 41 / Superkiller protein 6


Mass: 28007.129 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: SKI6, ECM20, RRP41, YGR195W, G7587 / Plasmid: pRSFduet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: P46948
#3: Protein Exosome complex component RRP43 / Ribosomal RNA-processing protein 43


Mass: 44060.934 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: RRP43, YCR035C, YCR35C, YCR522 / Plasmid: pRSFduet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: P25359
#4: Protein Exosome complex component RRP46 / Ribosomal RNA-processing protein 46


Mass: 24574.324 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: RRP46, YGR095C / Plasmid: pRSFduet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: P53256
#5: Protein Exosome complex component RRP42 / Ribosomal RNA-processing protein 42


Mass: 29492.574 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: RRP42, YDL111C / Plasmid: pRSFduet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: Q12277
#6: Protein Exosome complex component MTR3 / mRNA transport regulator 3


Mass: 27603.988 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: MTR3, YGR158C, G6676 / Plasmid: pRSFduet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: P48240
#7: Protein Exosome complex component RRP40 / Ribosomal RNA-processing protein 40


Mass: 26990.756 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: RRP40, YOL142W / Plasmid: pTOPO-Smt3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: Q08285
#8: Protein Exosome complex component RRP4 / Ribosomal RNA-processing protein 4


Mass: 39877.574 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: RRP4, YHR069C / Plasmid: pTOPO-Smt3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: P38792
#9: Protein Exosome complex component CSL4 / CEP1 synthetic lethal protein 4


Mass: 32026.684 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: CSL4, SKI4, YNL232W, N1154 / Plasmid: pTOPO-Smt3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: P53859

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Exosome complex exonuclease ... , 2 types, 2 molecules JK

#10: Protein Exosome complex exonuclease RRP6 / Ribosomal RNA-processing protein 6


Mass: 64515.590 Da / Num. of mol.: 1 / Mutation: D238N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: RRP6, UNC733, YOR001W / Plasmid: pTOPO-Smt3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL
References: UniProt: Q12149, Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters
#11: Protein Exosome complex exonuclease DIS3 / Chromosome disjunction protein 3 / Ribosomal RNA-processing protein 44


Mass: 114056.008 Da / Num. of mol.: 1 / Mutation: D171N, D551N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: DIS3, RRP44, YOL021C, O2197 / Plasmid: pTOPO-Smt3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL
References: UniProt: Q08162, Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters, Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters

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RNA chain , 1 types, 2 molecules ML

#12: RNA chain RNA (17-MER)


Mass: 5321.142 Da / Num. of mol.: 2 / Source method: obtained synthetically
Details: Original compound used for crystallization is 3'AAAAUUUUAUUUAUUAU5'-1,1'-(oxybis(ethane-2,1-diyl))bis(4-butyl-1H-1,2,3-triazole)-5'UAUUAUUUAUUUUAAAA3'
Source: (synth.) synthetic construct (others)

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Non-polymers , 4 types, 298 molecules

#13: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: SO4
#14: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C3H8O3
#15: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
#16: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 280 / Source method: isolated from a natural source / Formula: H2O

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Details

Compound detailsThe RNA chain in the crystal is two 17-mers linked head to head by (oxybis(ethane-2,1-diyl))bis(4- ...The RNA chain in the crystal is two 17-mers linked head to head by (oxybis(ethane-2,1-diyl))bis(4-butyl-1H-1,2,3-triazole) at their 5' ends to generate an RNA with two available 3' ends, 3'AAAAUUUUAUUUAUUAU5'-1,1'-(oxybis(ethane-2,1-diyl))bis(4-butyl-1H-1,2,3-triazole)-5'UAUUAUUUAUUUUAAAA3'. The crystallographic ASU includes two RNA chains that bridge the ASU to two other distinct ASUs. The linked 34-mer are intact in the crystal verified by analyzing the RNA after crystallization. The chemical bridge, and 10 nucleotides of one half of the 34mer RNA that is bound to Rrp44, are disordered. Densities are apparent along the path, but they were too ambiguous to be modeled.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.42 Å3/Da / Density % sol: 64.07 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.6
Details: 100 mM sodium citrate pH 5.25, 7 mM sodium MES pH 6.5, 6.7% PEG 3350, 175 mM (NH4)2SO4, 0.5 mM MgCl2, 40 mM TRIS-Cl pH 8.0, 200 mM NaCl, 2 mM TCEP-HCl

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9795 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 26, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 3.1→106.13 Å / Num. obs: 116037 / % possible obs: 98.8 % / Redundancy: 3.6 % / Biso Wilson estimate: 66.2 Å2 / Rmerge(I) obs: 0.116 / Net I/σ(I): 14.3
Reflection shellResolution: 3.1→3.21 Å / Redundancy: 3 % / Rmerge(I) obs: 0.534 / Mean I/σ(I) obs: 2.8 / % possible all: 97

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
PHENIX1.10_2155refinement
SCALEPACKdata scaling
PHASERphasing
PDB_EXTRACT3.2data extraction
Cootmodel building
HKL-2000data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4OO1, 2WP8

4oo1

2wp8


Resolution: 3.1→106.13 Å / SU ML: 0.42 / Cross valid method: THROUGHOUT / σ(F): 1 / Phase error: 26.22
Details: ITERATIVE ROUNDS OF REFINEMENT WERE ACCOMPLISHED USING PHENIX. RNA AND SIDE CHAINS WERE MANUALLY BUILT USING COOT. SIMULATED ANNEALING OMIT MAPS AND MAPS USED DURING BUILDING WERE ALSO ...Details: ITERATIVE ROUNDS OF REFINEMENT WERE ACCOMPLISHED USING PHENIX. RNA AND SIDE CHAINS WERE MANUALLY BUILT USING COOT. SIMULATED ANNEALING OMIT MAPS AND MAPS USED DURING BUILDING WERE ALSO GENERATED USING CNS. THE MODEL WAS REFINED USING POSITIONAL REFINEMENT, REAL-SPACE REFINEMENT AND INDIVIDUAL B-FACTOR REFINEMENT
RfactorNum. reflection% reflectionSelection details
Rfree0.249 5764 4.97 %Random
Rwork0.201 ---
obs0.203 116007 97.6 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 3.1→106.13 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms29376 502 93 280 30251
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00230520
X-RAY DIFFRACTIONf_angle_d0.42541404
X-RAY DIFFRACTIONf_dihedral_angle_d11.90518640
X-RAY DIFFRACTIONf_chiral_restr0.044798
X-RAY DIFFRACTIONf_plane_restr0.0035239
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.1-3.13530.36381920.30573580X-RAY DIFFRACTION96
3.1353-3.17210.37141660.30613638X-RAY DIFFRACTION97
3.1721-3.21080.32351890.2933631X-RAY DIFFRACTION97
3.2108-3.25150.37892080.29213616X-RAY DIFFRACTION98
3.2515-3.29430.33482070.27553600X-RAY DIFFRACTION97
3.2943-3.33940.30441800.25553640X-RAY DIFFRACTION98
3.3394-3.38710.28751900.2433634X-RAY DIFFRACTION98
3.3871-3.43770.30811890.24723655X-RAY DIFFRACTION98
3.4377-3.49140.31771860.2443657X-RAY DIFFRACTION97
3.4914-3.54860.33351620.23943631X-RAY DIFFRACTION97
3.5486-3.60980.29842100.22423649X-RAY DIFFRACTION97
3.6098-3.67550.27112030.2243566X-RAY DIFFRACTION97
3.6755-3.74620.25181850.21373576X-RAY DIFFRACTION95
3.7462-3.82260.29681690.20963484X-RAY DIFFRACTION93
3.8226-3.90580.29942240.20843651X-RAY DIFFRACTION98
3.9058-3.99660.26291800.19813698X-RAY DIFFRACTION99
3.9966-4.09660.24331970.19613706X-RAY DIFFRACTION99
4.0966-4.20730.26221990.18153692X-RAY DIFFRACTION99
4.2073-4.33110.20581930.16953718X-RAY DIFFRACTION99
4.3311-4.47090.22611940.16413745X-RAY DIFFRACTION99
4.4709-4.63070.1992190.15563694X-RAY DIFFRACTION99
4.6307-4.81610.19592120.15813718X-RAY DIFFRACTION99
4.8161-5.03530.2011680.16183569X-RAY DIFFRACTION95
5.0353-5.30080.20911420.16683775X-RAY DIFFRACTION99
5.3008-5.63290.21121940.17673783X-RAY DIFFRACTION99
5.6329-6.06780.26831990.18763773X-RAY DIFFRACTION99
6.0678-6.67830.27662000.19973771X-RAY DIFFRACTION99
6.6783-7.64440.19621880.19233657X-RAY DIFFRACTION96
7.6444-9.63020.19361950.16033847X-RAY DIFFRACTION99
9.6302-106.19070.2122240.20923889X-RAY DIFFRACTION97

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