+Open data
-Basic information
Entry | Database: PDB / ID: 5jvm | |||||||||
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Title | The neck-linker and alpha 7 helix of Mus musculus KIF3C | |||||||||
Components | Chimera protein of Kinesin-like protein KIF3C and Microtubule-associated protein RP/EB family member 1 | |||||||||
Keywords | MOTOR PROTEIN / kinesin / coiled-coil | |||||||||
Function / homology | Function and homology information microtubule motor activity => GO:0003777 / Intraflagellar transport / protein localization to astral microtubule / cortical microtubule cytoskeleton / mitotic spindle astral microtubule end / neuronal ribonucleoprotein granule / Kinesins / COPI-dependent Golgi-to-ER retrograde traffic / protein localization to microtubule / organelle transport along microtubule ...microtubule motor activity => GO:0003777 / Intraflagellar transport / protein localization to astral microtubule / cortical microtubule cytoskeleton / mitotic spindle astral microtubule end / neuronal ribonucleoprotein granule / Kinesins / COPI-dependent Golgi-to-ER retrograde traffic / protein localization to microtubule / organelle transport along microtubule / microtubule plus-end / cell projection membrane / attachment of mitotic spindle microtubules to kinetochore / MHC class II antigen presentation / microtubule plus-end binding / non-motile cilium assembly / microtubule bundle formation / protein localization to centrosome / microtubule organizing center / negative regulation of microtubule polymerization / kinesin complex / microtubule motor activity / microtubule-based movement / mitotic spindle pole / microtubule polymerization / establishment of mitotic spindle orientation / kinesin binding / spindle assembly / regulation of microtubule polymerization or depolymerization / spindle midzone / cytoplasmic microtubule / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / positive regulation of microtubule polymerization / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Resolution of Sister Chromatid Cohesion / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / ciliary basal body / RHO GTPases Activate Formins / protein localization / positive regulation of neuron projection development / Separation of Sister Chromatids / The role of GTSE1 in G2/M progression after G2 checkpoint / microtubule cytoskeleton / Regulation of PLK1 Activity at G2/M Transition / cell migration / synaptic vesicle / growth cone / microtubule binding / microtubule / molecular adaptor activity / cadherin binding / cell division / axon / focal adhesion / centrosome / dendrite / neuronal cell body / protein kinase binding / Golgi apparatus / ATP hydrolysis activity / RNA binding / ATP binding / membrane / identical protein binding / cytosol Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.567 Å | |||||||||
Authors | Phillips, R.K. / Rayment, I. | |||||||||
Funding support | United States, 2items
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Citation | Journal: J.Biol.Chem. / Year: 2016 Title: Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil. Authors: Phillips, R.K. / Peter, L.G. / Gilbert, S.P. / Rayment, I. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5jvm.cif.gz | 88.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5jvm.ent.gz | 67.4 KB | Display | PDB format |
PDBx/mmJSON format | 5jvm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jv/5jvm ftp://data.pdbj.org/pub/pdb/validation_reports/jv/5jvm | HTTPS FTP |
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-Related structure data
Related structure data | 5jv3C 5jvpC 5jvrC 5jvsC 5jvuC 5jx1C 1yibS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 9638.826 Da / Num. of mol.: 2 Fragment: UNP O35066 residues 374-402,UNP Q15691 residues 207-257 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse), (gene. exp.) Homo sapiens (human) Gene: Kif3c, MAPRE1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: O35066, UniProt: Q15691 #2: Chemical | ChemComp-MG / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.88 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 9 Details: 30% (w/v) pentaerythritol ethoxylate (PEE) 797, 1.5% (w/v) ethylene glycol monoethylether, 400 mM MgCl2, 100 mM bis-tris propane pH 9.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97924 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 13, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97924 Å / Relative weight: 1 |
Reflection | Resolution: 1.5667→37.004 Å / Num. obs: 44738 / % possible obs: 98 % / Redundancy: 7.1 % / Rmerge(I) obs: 0.073 / Net I/σ(I): 21.7 |
Reflection shell | Resolution: 1.5667→1.62 Å / Redundancy: 3 % / Rmerge(I) obs: 0.595 / Mean I/σ(I) obs: 1.6 / Num. measured obs: 2513 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1YIB Resolution: 1.567→37.004 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 22.42 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.567→37.004 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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