Entry Database : PDB / ID : 5jqh Structure visualization Downloads & linksTitle Structure of beta2 adrenoceptor bound to carazolol and inactive-state stabilizing nanobody, Nb60 ComponentsEndolysin,Beta-2 adrenergic receptor Nanobody60, Nb60 DetailsKeywords signaling protein / hydrolase/inhibitor / GPCR / Signaling / Nanobody / Allostery / HYDROLASE / hydrolase-inhibitor complexFunction / homology Function and homology informationFunction Domain/homology Component
: / beta2-adrenergic receptor activity / positive regulation of mini excitatory postsynaptic potential / positive regulation of cAMP-dependent protein kinase activity / norepinephrine binding / Adrenoceptors / heat generation / positive regulation of autophagosome maturation / positive regulation of AMPA receptor activity / norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure ... : / beta2-adrenergic receptor activity / positive regulation of mini excitatory postsynaptic potential / positive regulation of cAMP-dependent protein kinase activity / norepinephrine binding / Adrenoceptors / heat generation / positive regulation of autophagosome maturation / positive regulation of AMPA receptor activity / norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure / activation of transmembrane receptor protein tyrosine kinase activity / negative regulation of smooth muscle contraction / positive regulation of lipophagy / response to psychosocial stress / negative regulation of multicellular organism growth / adrenergic receptor signaling pathway / endosome to lysosome transport / diet induced thermogenesis / neuronal dense core vesicle / positive regulation of protein kinase A signaling / adenylate cyclase binding / smooth muscle contraction / potassium channel regulator activity / positive regulation of bone mineralization / brown fat cell differentiation / adenylate cyclase-activating adrenergic receptor signaling pathway / regulation of sodium ion transport / viral release from host cell by cytolysis / bone resorption / activation of adenylate cyclase activity / response to cold / peptidoglycan catabolic process / receptor-mediated endocytosis / clathrin-coated endocytic vesicle membrane / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / positive regulation of protein serine/threonine kinase activity / cellular response to amyloid-beta / cell wall macromolecule catabolic process / Cargo recognition for clathrin-mediated endocytosis / lysozyme / Clathrin-mediated endocytosis / lysozyme activity / positive regulation of cold-induced thermogenesis / amyloid-beta binding / G alpha (s) signalling events / host cell cytoplasm / transcription by RNA polymerase II / positive regulation of MAPK cascade / cell surface receptor signaling pathway / lysosome / early endosome / receptor complex / endosome membrane / Ub-specific processing proteases / endosome / defense response to bacterium / apical plasma membrane / protein-containing complex binding / Golgi apparatus / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / identical protein binding / membrane / nucleus / plasma membrane Similarity search - Function Beta 2 adrenoceptor / Adrenoceptor family / Rhopdopsin 7-helix transmembrane proteins / Rhodopsin 7-helix transmembrane proteins / Lysozyme - #40 / Endolysin T4 type / T4-type lysozyme / Glycoside hydrolase, family 24 / Lysozyme domain superfamily / Phage lysozyme ... Beta 2 adrenoceptor / Adrenoceptor family / Rhopdopsin 7-helix transmembrane proteins / Rhodopsin 7-helix transmembrane proteins / Lysozyme - #40 / Endolysin T4 type / T4-type lysozyme / Glycoside hydrolase, family 24 / Lysozyme domain superfamily / Phage lysozyme / Serpentine type 7TM GPCR chemoreceptor Srsx / Lysozyme / G-protein coupled receptors family 1 signature. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / 7 transmembrane receptor (rhodopsin family) / Lysozyme-like domain superfamily / Immunoglobulins / Up-down Bundle / Immunoglobulin-like / Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha Similarity search - Domain/homologyBiological species Enterobacteria phage T4 (virus)Homo sapiens (human)Lama glama (llama)Method X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution : 3.2 Å DetailsAuthors Staus, D.P. / Strachan, R.T. / Manglik, A. / Pani, B. / Kahsai, A.W. / Kim, T.H. / Wingler, L.M. / Ahn, S. / Chatterjee, A. / Masoudi, A. ...Staus, D.P. / Strachan, R.T. / Manglik, A. / Pani, B. / Kahsai, A.W. / Kim, T.H. / Wingler, L.M. / Ahn, S. / Chatterjee, A. / Masoudi, A. / Kruse, A.C. / Pardon, E. / Steyaert, J. / Weis, W.I. / Prosser, R.S. / Kobilka, B.K. / Costa, T. / Lefkowitz, R.J. Funding support United States, 2items Details Hide detailsOrganization Grant number Country National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) HL70631 United States National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) NS028471 United States
CitationJournal : Nature / Year : 2016Title : Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation.Authors: Staus, D.P. / Strachan, R.T. / Manglik, A. / Pani, B. / Kahsai, A.W. / Kim, T.H. / Wingler, L.M. / Ahn, S. / Chatterjee, A. / Masoudi, A. / Kruse, A.C. / Pardon, E. / Steyaert, J. / Weis, W. ... Authors : Staus, D.P. / Strachan, R.T. / Manglik, A. / Pani, B. / Kahsai, A.W. / Kim, T.H. / Wingler, L.M. / Ahn, S. / Chatterjee, A. / Masoudi, A. / Kruse, A.C. / Pardon, E. / Steyaert, J. / Weis, W.I. / Prosser, R.S. / Kobilka, B.K. / Costa, T. / Lefkowitz, R.J. History Deposition May 5, 2016 Deposition site : RCSB / Processing site : RCSBRevision 1.0 Jul 13, 2016 Provider : repository / Type : Initial releaseRevision 1.1 Jul 27, 2016 Group : Database referencesRevision 1.2 Aug 3, 2016 Group : Database referencesRevision 1.3 Sep 20, 2017 Group : Author supporting evidence / Database references / Derived calculationsCategory : citation / pdbx_audit_support / pdbx_struct_oper_listItem : _citation.journal_id_CSD / _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operationRevision 1.4 Dec 4, 2019 Group : Author supporting evidence / Category : pdbx_audit_support / Item : _pdbx_audit_support.funding_organizationRevision 1.5 Sep 27, 2023 Group : Data collection / Database references / Refinement descriptionCategory : chem_comp_atom / chem_comp_bond ... chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession
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