Mass: 18.015 Da / Num. of mol.: 381 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.87 Å3/Da / Density % sol: 57.1 %
Crystal grow
Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 20 to 22% poly(ethylene glycol) 5000, 50 mM MgCl2, 5 mM N5-formyltetrahydrofolate, 10 mM UDP-Ara4N, and 100 mM HEPPS (pH 8.0)
Resolution: 2.5→20 Å / Cor.coef. Fo:Fc: 0.921 / Cor.coef. Fo:Fc free: 0.853 / SU B: 12.339 / SU ML: 0.271 / Cross valid method: THROUGHOUT / ESU R: 0.858 / ESU R Free: 0.354 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.28404
2253
4.9 %
RANDOM
Rwork
0.21213
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obs
0.21571
43477
91.25 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å