- PDB-5j3n: C-terminal domain of EcoR124I HsdR subunit fused with the pH-sens... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 5j3n
Title
C-terminal domain of EcoR124I HsdR subunit fused with the pH-sensitive GFP variant ratiometric pHluorin
Components
Green fluorescent protein,HsdR
Keywords
HYDROLASE / HsdR / EcoR124I / type I restriction-modification system / pHluorin / GFP / fusion protein
Function / homology
Function and homology information
type I site-specific deoxyribonuclease / type I site-specific deoxyribonuclease activity / DNA restriction-modification system / serine-type endopeptidase inhibitor activity / DNA binding / extracellular space / ATP binding / metal ion binding Similarity search - Function
: / Restriction endonuclease, type I, HsdR, N-terminal / Type I restriction enzyme R protein, C-terminal / SWI2/SNF2 ATPase / Type I restriction enzyme R protein N terminus (HSDR_N) / Type I restriction and modification enzyme - subunit R C terminal / SWI2/SNF2 ATPase / Restriction endonuclease, type I, HsdR / Green Fluorescent Protein / Green fluorescent protein ...: / Restriction endonuclease, type I, HsdR, N-terminal / Type I restriction enzyme R protein, C-terminal / SWI2/SNF2 ATPase / Type I restriction enzyme R protein N terminus (HSDR_N) / Type I restriction and modification enzyme - subunit R C terminal / SWI2/SNF2 ATPase / Restriction endonuclease, type I, HsdR / Green Fluorescent Protein / Green fluorescent protein / Serpin, conserved site / Serpins signature. / Serpin superfamily, domain 2 / Serpin family / Serpin domain / Serpin superfamily / Serpin superfamily, domain 1 / Serpin (serine protease inhibitor) / SERine Proteinase INhibitors / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase superfamily 1/2, ATP-binding domain / Beta Barrel / P-loop containing nucleoside triphosphate hydrolase / Mainly Beta Similarity search - Domain/homology
Resolution: 2.45→20 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.904 / SU B: 9.955 / SU ML: 0.21 / Cross valid method: THROUGHOUT / ESU R: 0.302 / ESU R Free: 0.248 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.25383
1716
5 %
RANDOM
Rwork
0.19194
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obs
0.1949
32725
99.19 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å