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Open data
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Basic information
Entry | Database: PDB / ID: 5iwn | |||||||||
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Title | Bacterial sodium channel pore domain, high bromide | |||||||||
![]() | Ion transport protein | |||||||||
![]() | TRANSPORT PROTEIN / Bacterial sodium channel / low Br | |||||||||
Function / homology | ![]() voltage-gated sodium channel complex / membrane depolarization during action potential / voltage-gated sodium channel activity / identical protein binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Shaya, D. / Findeisen, F. / Rohaim, A. / Minor, D.L. | |||||||||
![]() | ![]() Title: Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Authors: Arrigoni, C. / Rohaim, A. / Shaya, D. / Findeisen, F. / Stein, R.A. / Nurva, S.R. / Mishra, S. / Mchaourab, H.S. / Minor, D.L. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 121.7 KB | Display | ![]() |
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PDB format | ![]() | 96.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 457.2 KB | Display | ![]() |
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Full document | ![]() | 482 KB | Display | |
Data in XML | ![]() | 23.8 KB | Display | |
Data in CIF | ![]() | 31.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5hj8C ![]() 5hk6C ![]() 5hk7C ![]() 5hkdC ![]() 5iwoC ![]() 4ltoS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17463.344 Da / Num. of mol.: 4 / Fragment: UNP residues 143-288 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 7.58 Å3/Da / Density % sol: 83.8 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.25 / Details: 100 mM sodium acetate, pH 5.25, 30% PEG300 |
-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Dec 6, 2014 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 3.75→15 Å / Num. obs: 21081 / % possible obs: 99.1 % / Redundancy: 4.9 % / Rsym value: 0.258 / Net I/σ(I): 5.4 |
Reflection shell | Highest resolution: 3.75 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 4LTO Resolution: 3.75→15 Å / SU ML: 0.422 / Cross valid method: FREE R-VALUE / ESU R: 1.1 / ESU R Free: 0.499
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||
Displacement parameters | Biso mean: 168 Å2
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Refinement step | Cycle: LAST / Resolution: 3.75→15 Å
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LS refinement shell | Resolution: 3.75→3.84 Å
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