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- PDB-5isz: Crystal structure of LS01-TCR/M1-HLA-A*02 complex -

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Basic information

Entry
Database: PDB / ID: 5isz
TitleCrystal structure of LS01-TCR/M1-HLA-A*02 complex
Components
  • Beta-2-microglobulin
  • HLA class I histocompatibility antigen, A-2 alpha chain
  • TCRalpha chain
  • TCRbeta chain
  • influenza M1 for peptide
KeywordsIMMUNE SYSTEM / TCR-HLA*A2 / M1 / influenza / matrix protein
Function / homology
Function and homology information


Assembly of Viral Components at the Budding Site / Influenza Infection / Fusion of the Influenza Virion to the Host Cell Endosome / Release / Budding / Packaging of Eight RNA Segments / Uncoating of the Influenza Virion / Entry of Influenza Virion into Host Cell via Endocytosis / Viral RNP Complexes in the Host Cell Nucleus / NEP/NS2 Interacts with the Cellular Export Machinery ...Assembly of Viral Components at the Budding Site / Influenza Infection / Fusion of the Influenza Virion to the Host Cell Endosome / Release / Budding / Packaging of Eight RNA Segments / Uncoating of the Influenza Virion / Entry of Influenza Virion into Host Cell via Endocytosis / Viral RNP Complexes in the Host Cell Nucleus / NEP/NS2 Interacts with the Cellular Export Machinery / T cell mediated cytotoxicity directed against tumor cell target / positive regulation of memory T cell activation / TAP complex binding / positive regulation of CD8-positive, alpha-beta T cell activation / CD8-positive, alpha-beta T cell activation / Golgi medial cisterna / positive regulation of CD8-positive, alpha-beta T cell proliferation / CD8 receptor binding / antigen processing and presentation of exogenous peptide antigen via MHC class I / endoplasmic reticulum exit site / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent / Viral mRNA Translation / TAP binding / protection from natural killer cell mediated cytotoxicity / beta-2-microglobulin binding / T cell receptor binding / detection of bacterium / viral budding from plasma membrane / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / lumenal side of endoplasmic reticulum membrane / cellular response to iron ion / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / regulation of erythrocyte differentiation / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of iron ion transport / response to molecule of bacterial origin / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / negative regulation of neurogenesis / peptide antigen assembly with MHC class II protein complex / positive regulation of receptor-mediated endocytosis / MHC class II protein complex / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / positive regulation of cellular senescence / peptide antigen binding / negative regulation of epithelial cell proliferation / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / Modulation by Mtb of host immune system / Interferon alpha/beta signaling / positive regulation of type II interferon production / sensory perception of smell / positive regulation of T cell activation / positive regulation of protein binding / tertiary granule lumen / E3 ubiquitin ligases ubiquitinate target proteins / DAP12 signaling / negative regulation of neuron projection development / MHC class II protein complex binding / late endosome membrane / T cell receptor signaling pathway / iron ion transport / ER-Phagosome pathway / early endosome membrane / antibacterial humoral response / T cell differentiation in thymus / protein refolding / protein homotetramerization / intracellular iron ion homeostasis / amyloid fibril formation / structural constituent of virion / learning or memory / defense response to Gram-positive bacterium / immune response / Amyloid fiber formation / endoplasmic reticulum lumen
Similarity search - Function
Matrix protein 1 / Influenza matrix M1, N-terminal / Influenza matrix M1, C-terminal / Influenza matrix M1, N-terminal subdomain 1 / Influenza matrix M1, N-terminal subdomain 2 / Influenza virus matrix protein M1 / Influenza Matrix protein (M1) / Influenza Matrix protein (M1) C-terminal domain / Influenza Matrix protein (M1) C-terminal domain / MHC class I, alpha chain, C-terminal ...Matrix protein 1 / Influenza matrix M1, N-terminal / Influenza matrix M1, C-terminal / Influenza matrix M1, N-terminal subdomain 1 / Influenza matrix M1, N-terminal subdomain 2 / Influenza virus matrix protein M1 / Influenza Matrix protein (M1) / Influenza Matrix protein (M1) C-terminal domain / Influenza Matrix protein (M1) C-terminal domain / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
HLA class I histocompatibility antigen, A alpha chain / Matrix protein 1 / HLA class I histocompatibility antigen, A alpha chain / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
Influenza A virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.06 Å
AuthorsStern, L.J. / Selin, L.K. / Song, I.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI038996 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI109858 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI49320 United States
CitationJournal: Nat. Struct. Mol. Biol. / Year: 2017
Title: Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8(+) T cell epitope.
Authors: Song, I. / Gil, A. / Mishra, R. / Ghersi, D. / Selin, L.K. / Stern, L.J.
History
DepositionMar 15, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 1, 2017Provider: repository / Type: Initial release
Revision 1.1Mar 15, 2017Group: Database references
Revision 1.2Apr 19, 2017Group: Database references
Revision 1.3Sep 20, 2017Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4Dec 11, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.5Sep 27, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HLA class I histocompatibility antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: influenza M1 for peptide
D: TCRalpha chain
E: TCRbeta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)94,3078
Polymers94,0305
Non-polymers2763
Water10,863603
1


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10900 Å2
ΔGint-56 kcal/mol
Surface area38380 Å2
MethodPISA
Unit cell
Length a, b, c (Å)63.810, 75.460, 121.200
Angle α, β, γ (deg.)90.00, 98.01, 90.00
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 4 types, 4 molecules ABDE

#1: Protein HLA class I histocompatibility antigen, A-2 alpha chain / MHC class I antigen A*2


Mass: 31854.203 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-A, HLAA / Plasmid: pML1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P01892, UniProt: P04439*PLUS
#2: Protein Beta-2-microglobulin


Mass: 11635.002 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Plasmid: pML1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P61769
#4: Protein TCRalpha chain


Mass: 22189.375 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRA@ / Production host: Escherichia coli (E. coli)
#5: Protein TCRbeta chain


Mass: 27385.510 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)

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Protein/peptide , 1 types, 1 molecules C

#3: Protein/peptide influenza M1 for peptide


Mass: 966.174 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: chemical synthesis / Source: (synth.) Influenza A virus / References: UniProt: P03485*PLUS

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Non-polymers , 2 types, 606 molecules

#6: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H8O3
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 603 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.07 Å3/Da / Density % sol: 59.97 % / Mosaicity: 1.27 °
Crystal growTemperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 14% (w/v) PEG 4000, 100 mM Na-HEPES pH 7.0, 200 mM ammonium sulfate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.9793 Å
DetectorType: RAYONIX MX225HE / Detector: CCD / Date: Apr 17, 2015
RadiationMonochromator: Kohzu HLD-4 Double Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9793 Å / Relative weight: 1
ReflectionResolution: 2.06→120.066 Å / Num. obs: 65704 / % possible obs: 92.9 % / Redundancy: 5.7 % / Rsym value: 0.178 / Net I/av σ(I): 2.93 / Net I/σ(I): 5.6
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsDiffraction-ID% possible all
2.06-2.175.50.491.5184.9
2.17-2.35.30.4241.7188.3
2.3-2.465.30.3542190.8
2.46-2.665.30.2782.5193.3
2.66-2.915.40.2392.8195
2.91-3.265.60.2093.1196
3.26-3.7660.1843.4197.5
3.76-4.616.70.1574199.5
4.61-6.517.10.1234.91100
6.51-36.0256.80.0778.4198.5

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation7.57 Å35.99 Å
Translation7.57 Å35.99 Å

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Processing

Software
NameVersionClassification
PHENIX(1.10.1_2155: ???)refinement
iMOSFLMdata reduction
SCALA3.3.22data scaling
PHASER2.5.6phasing
PDB_EXTRACT3.2data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1OGA

1oga


Resolution: 2.06→29.143 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.66 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.1999 2841 5 %
Rwork0.1838 --
obs0.1846 56793 80.45 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.06→29.143 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6611 0 18 603 7232
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0056828
X-RAY DIFFRACTIONf_angle_d0.6929277
X-RAY DIFFRACTIONf_dihedral_angle_d14.8344046
X-RAY DIFFRACTIONf_chiral_restr0.048966
X-RAY DIFFRACTIONf_plane_restr0.0051212
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.06-2.09550.27911180.2362309X-RAY DIFFRACTION69
2.0955-2.13360.23311140.22842286X-RAY DIFFRACTION69
2.1336-2.17470.2361240.21312280X-RAY DIFFRACTION69
2.1747-2.2190.23591290.20892286X-RAY DIFFRACTION68
2.219-2.26730.23971260.21662319X-RAY DIFFRACTION70
2.2673-2.320.26141130.21912311X-RAY DIFFRACTION70
2.32-2.3780.25061230.21912408X-RAY DIFFRACTION71
2.378-2.44220.25391240.22382400X-RAY DIFFRACTION72
2.4422-2.51410.27951240.21682445X-RAY DIFFRACTION73
2.5141-2.59520.22691350.21572495X-RAY DIFFRACTION75
2.5952-2.68790.24031320.21652566X-RAY DIFFRACTION77
2.6879-2.79540.21641400.22342657X-RAY DIFFRACTION80
2.7954-2.92250.23991540.21352754X-RAY DIFFRACTION83
2.9225-3.07640.27211590.21542860X-RAY DIFFRACTION86
3.0764-3.26890.17831630.19562985X-RAY DIFFRACTION89
3.2689-3.52090.19581650.18483129X-RAY DIFFRACTION93
3.5209-3.87450.18891550.1643278X-RAY DIFFRACTION97
3.8745-4.43350.15411820.13913351X-RAY DIFFRACTION99
4.4335-5.57930.13731830.13563385X-RAY DIFFRACTION100
5.5793-29.14550.19551780.18393448X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.2882-0.17070.96111.5094-0.41422.6807-0.0783-0.06840.31020.1021-0.0107-0.111-0.217-0.04210.04710.14490.00540.00070.0724-0.00360.182747.3147-29.698950.1589
22.42820.2061.03131.8937-0.71192.20020.13350.2448-0.5947-0.3602-0.0216-0.3010.55290.0095-0.15180.25140.00070.02110.1356-0.04170.26250.0773-46.479148.1181
31.5779-0.20511.33961.6965-1.43053.75330.0367-0.0101-0.28980.01040.0053-0.07270.3738-0.1475-0.13080.19140.00280.00990.0979-0.0270.243443.1794-44.530552.9857
44.1786-0.9604-1.63942.51941.25934.7625-0.00680.5761-0.2575-0.44120.1808-0.31830.48520.56180.00270.37220.009-0.00040.4509-0.08010.255553.9792-37.436116.6248
55.0223-0.24545.36951.79040.10835.8944-0.0756-0.0072-0.1228-0.0129-0.0414-0.26810.4114-0.0838-0.18610.15510.00720.04020.34990.0290.258861.1438-27.462631.8383
62.5932-0.96890.80724.2987-2.70843.78810.05450.43220.1586-0.38050.03880.0541-0.0232-0.0657-0.0780.1652-0.00130.01610.3370.0150.193851.1215-20.599624.2233
73.4932-1.95752.77764.0351-0.46853.7453-0.16760.24430.3950.7092-0.5452-0.4258-0.87360.57240.7030.102-0.0079-0.02340.23380.04680.26660.5715-19.270336.989
82.48351.1307-2.73531.7047-3.59367.5140.29040.11610.59060.8723-0.11660.1334-1.4088-0.1989-0.28790.2345-0.0194-0.03720.22580.03660.476953.9519-16.129135.179
97.9772-1.7881.54317.8013-3.71631.83250.11270.4375-0.7729-0.4094-0.3793-0.77711.1480.41310.14020.26910.00810.01140.1051-0.04190.369756.9973-37.936241.132
101.8805-0.16630.35177.4522-5.87375.1835-0.1310.65480.2038-0.2169-0.2164-0.2049-0.30960.29090.15240.2048-0.0309-0.02690.32180.08820.26553.6759-16.825826.2947
112.7613-4.27664.35917.1186-6.78427.2653-0.20970.22090.3695-0.07160.040.1315-0.64180.27020.25080.1842-0.0518-0.01080.32260.1050.353562.1289-16.397430.1292
125.3358-3.76925.71116.8626-1.42678.44960.0520.12720.1893-0.1525-0.2781-0.234-0.33360.13240.04930.1534-0.02920.0240.16240.00330.22870.1439-22.237137.9673
137.2980.60134.57386.89121.30265.37710.27241.3828-0.0832-1.28360.1298-0.4914-0.17990.4662-0.220.32240.05270.10840.63590.0530.263161.1387-20.556120.4965
142.2281-0.949-0.22690.4310.09970.014-0.2884-0.41820.1490.07060.1638-0.0541-0.1074-0.16330.17970.1429-0.0002-0.01630.1768-0.03340.2745.0857-37.925857.7433
152.28682.0382-2.71242.2642-1.64193.85520.07210.03580.19040.05260.16330.4434-0.2764-0.6266-0.23610.18090.050.00950.26550.01050.289927.5882-50.377774.0795
161.99632.0134-1.46993.6439-1.38186.2288-0.06320.0593-0.051-0.33240.05180.10380.4196-0.26980.09030.1156-0.02520.00910.24-0.0370.250829.5247-58.365771.6343
171.4032.0149-2.70122.779-3.97877.9180.15730.16570.20190.25710.10170.4406-0.6237-0.7234-0.22140.21410.04050.02290.18720.00270.271830.155-43.440369.4423
183.42732.4711-4.44612.5666-3.34186.35860.1916-0.24990.08220.29540.09-0.22860.37750.2337-0.27480.3762-0.0784-0.00480.4049-0.10780.210321.089-59.162494.4356
191.89730.11730.17392.2013-0.09640.02160.1974-1.0351.03071.60190.0219-0.02890.107-0.2786-0.10950.593-0.13320.01091.1275-0.22560.548919.7717-43.2617116.5095
208.5164-2.59120.76337.1514-2.13287.6909-0.04180.11290.01880.2106-0.1270.43220.06480.12880.1580.2706-0.11330.04920.4214-0.04570.226215.241-52.1991102.2092
216.6153-3.11030.92383.4252-0.73884.40240.4515-0.03440.3936-0.3012-0.4575-0.11640.0309-0.21210.10780.2559-0.08470.0140.4248-0.13430.306221.5324-50.4848102.2341
223.29220.46210.50274.0052-2.38255.19930.3703-0.95690.59221.0909-0.28720.4328-0.3469-0.197-0.43770.4019-0.08450.05730.598-0.19830.39869.3299-48.6551109.1279
233.1340.691-0.78870.8211-0.36474.08050.012-0.61380.47050.7704-0.08770.0917-0.4662-0.2183-0.09370.5495-0.04470.02170.3544-0.1170.299738.9412-27.983585.4305
241.7859-0.4431-0.73835.04241.06884.18530.05030.01180.18510.2583-0.13840.4024-0.565-0.21610.05160.2322-0.00610.00760.1744-0.02960.197137.2289-31.898874.0871
253.71120.5053-0.45170.07840.11154.5007-0.02360.32460.64140.1627-0.10150.5509-0.6974-0.4908-0.10160.83710.02650.25690.3928-0.23870.541127.5625-23.599795.2803
262.21780.8771-0.98793.0352-0.14491.74650.2897-0.6820.1190.3961-0.1151-0.0898-0.24670.0962-0.21680.353-0.09-0.01540.5404-0.1110.22327.2002-44.0624108.8061
272.31520.0063-0.02084.984-0.84992.16590.2207-0.37190.29190.1493-0.0018-0.119-0.56060.165-0.1820.4515-0.11790.09040.5142-0.16930.270130.7135-37.4489106.5191
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 103 )
2X-RAY DIFFRACTION2chain 'A' and (resid 104 through 137 )
3X-RAY DIFFRACTION3chain 'A' and (resid 138 through 174 )
4X-RAY DIFFRACTION4chain 'A' and (resid 175 through 275 )
5X-RAY DIFFRACTION5chain 'B' and (resid 2 through 11 )
6X-RAY DIFFRACTION6chain 'B' and (resid 12 through 30 )
7X-RAY DIFFRACTION7chain 'B' and (resid 31 through 41 )
8X-RAY DIFFRACTION8chain 'B' and (resid 42 through 56 )
9X-RAY DIFFRACTION9chain 'B' and (resid 57 through 61 )
10X-RAY DIFFRACTION10chain 'B' and (resid 62 through 77 )
11X-RAY DIFFRACTION11chain 'B' and (resid 78 through 83 )
12X-RAY DIFFRACTION12chain 'B' and (resid 84 through 90 )
13X-RAY DIFFRACTION13chain 'B' and (resid 91 through 99 )
14X-RAY DIFFRACTION14chain 'C' and (resid 1 through 9 )
15X-RAY DIFFRACTION15chain 'D' and (resid 2 through 50 )
16X-RAY DIFFRACTION16chain 'D' and (resid 51 through 85 )
17X-RAY DIFFRACTION17chain 'D' and (resid 86 through 104 )
18X-RAY DIFFRACTION18chain 'D' and (resid 105 through 118 )
19X-RAY DIFFRACTION19chain 'D' and (resid 119 through 131 )
20X-RAY DIFFRACTION20chain 'D' and (resid 132 through 147 )
21X-RAY DIFFRACTION21chain 'D' and (resid 148 through 174 )
22X-RAY DIFFRACTION22chain 'D' and (resid 175 through 201 )
23X-RAY DIFFRACTION23chain 'E' and (resid 3 through 27 )
24X-RAY DIFFRACTION24chain 'E' and (resid 28 through 108 )
25X-RAY DIFFRACTION25chain 'E' and (resid 109 through 123 )
26X-RAY DIFFRACTION26chain 'E' and (resid 124 through 161 )
27X-RAY DIFFRACTION27chain 'E' and (resid 162 through 243 )

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