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- PDB-5iqj: 1.9 Angstrom Crystal Structure of Protein with Unknown Function f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5iqj | ||||||
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Title | 1.9 Angstrom Crystal Structure of Protein with Unknown Function from Vibrio cholerae. | ||||||
![]() | Uncharacterized protein | ||||||
![]() | UNKNOWN FUNCTION / CSGID / Vibrio cholerae / Structural Genomics / Center for Structural Genomics of Infectious Diseases | ||||||
Function / homology | ACETATE ION / DI(HYDROXYETHYL)ETHER / TRIETHYLENE GLYCOL / TARTRONATE / Uncharacterized protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Minasov, G. / Wawrzak, Z. / Stogios, P.J. / Skarina, T. / Seed, K.D. / Yim, V. / Savchenko, A. / Anderson, W.F. / Center for Structural Genomics of Infectious Diseases (CSGID) | ||||||
![]() | ![]() Title: 1.9 Angstrom Crystal Structure of Protein with Unknown Function from Vibrio cholerae. Authors: Minasov, G. / Wawrzak, Z. / Stogios, P.J. / Skarina, T. / Seed, K.D. / Yim, V. / Savchenko, A. / Anderson, W.F. / Center for Structural Genomics of Infectious Diseases | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 186.6 KB | Display | ![]() |
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PDB format | ![]() | 150.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 521.8 KB | Display | ![]() |
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Full document | ![]() | 526.8 KB | Display | |
Data in XML | ![]() | 22.2 KB | Display | |
Data in CIF | ![]() | 32.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 3 molecules ABC
#1: Protein | Mass: 15042.494 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Plasmid: pMCSG53 / Production host: ![]() ![]() |
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-Non-polymers , 9 types, 417 molecules ![](data/chem/img/CL.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/TTN.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/TTN.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CL / #3: Chemical | ChemComp-SO4 / #4: Chemical | #5: Chemical | #6: Chemical | ChemComp-PEG / | #7: Chemical | ChemComp-PGE / | #8: Chemical | ChemComp-ACT / | #9: Chemical | ChemComp-GOL / | #10: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.1 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Protein: 7.0 mg/ml, 0.3M Sodium chloride, 0.01M HEPES (pH 7.5); Screen: 2M Ammonium sulfate, 0.1M HEPES (pH 7.5), 2% (w/v) PEG 400, 1% P300; |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 18, 2015 / Details: C(111) |
Radiation | Monochromator: beryllium lenses / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97872 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→29.41 Å / Num. obs: 42909 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 5.2 % / Biso Wilson estimate: 28.8 Å2 / CC1/2: 0.873 / Rmerge(I) obs: 0.067 / Rsym value: 0.067 / Net I/σ(I): 20.3 |
Reflection shell | Resolution: 1.9→1.93 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.586 / Mean I/σ(I) obs: 2.9 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.364 Å2
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Refinement step | Cycle: 1 / Resolution: 1.9→29.41 Å
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Refine LS restraints |
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