Mass: 18.015 Da / Num. of mol.: 19 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.55 Å3/Da / Density % sol: 51.69 % / Description: Thin plates
Crystal grow
Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 7.25 Details: F-BARx-WT was eluted from the anion exchange column (MonoQ-GL10/100, GE Healthcare) in buffer containing 5% Glycerol, 25 mM Tris buffer pH=7.4, 5 mM ?-mercaptoethanol, and ~0.16 M NaCl at ...Details: F-BARx-WT was eluted from the anion exchange column (MonoQ-GL10/100, GE Healthcare) in buffer containing 5% Glycerol, 25 mM Tris buffer pH=7.4, 5 mM ?-mercaptoethanol, and ~0.16 M NaCl at the elution peak and concentrated to 18 mg/ml. F-BARx-WT crystals grew in 0.2 M ammonium citrate tribasic pH=7.25, 35% PEG1500.