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Open data
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Basic information
Entry | Database: PDB / ID: 5htl | ||||||
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Title | Structure of MshE with cdg | ||||||
![]() | MSHA biogenesis protein MshE | ||||||
![]() | BIOSYNTHETIC PROTEIN / MshE / c-di-GMP | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Chin, K.H. / Wang, Y.C. | ||||||
![]() | ![]() Title: Nucleotide binding by the widespread high-affinity cyclic di-GMP receptor MshEN domain. Authors: Wang, Y.C. / Chin, K.H. / Tu, Z.L. / He, J. / Jones, C.J. / Sanchez, D.Z. / Yildiz, F.H. / Galperin, M.Y. / Chou, S.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 136.4 KB | Display | ![]() |
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PDB format | ![]() | 111.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 16291.353 Da / Num. of mol.: 2 / Fragment: UNP RESIDUES 1-145 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 35.54 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: Tris 100mM pH 8.0, PEG 3350 20% |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX325HE / Detector: CCD / Date: Nov 8, 2015 |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97902 Å / Relative weight: 1 |
Reflection | Resolution: 1.37→50 Å / Num. obs: 234156 / % possible obs: 95.4 % / Redundancy: 4.6 % / Net I/σ(I): 18.2 |
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Processing
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Refinement | Resolution: 1.371→26.426 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 22.45
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Bsol: 53.696 Å2 / ksol: 0.425 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.371→26.426 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -0.9985 Å / Origin y: -6.2351 Å / Origin z: 2.0627 Å
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Refinement TLS group | Selection details: all |