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Yorodumi- PDB-5gr3: Crystal structure of branching enzyme L541A/W655A mutant from Cya... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5gr3 | ||||||||||||
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Title | Crystal structure of branching enzyme L541A/W655A mutant from Cyanothece sp. ATCC 51142 | ||||||||||||
Components | 1,4-alpha-glucan branching enzyme GlgB | ||||||||||||
Keywords | TRANSFERASE / branching enzyme / glycoside hydrolase family 13 / cyanobacteria / starch | ||||||||||||
Function / homology | Function and homology information 1,4-alpha-glucan branching enzyme / 1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis) / 1,4-alpha-glucan branching enzyme activity / cation binding / glycogen biosynthetic process / hydrolase activity, hydrolyzing O-glycosyl compounds Similarity search - Function | ||||||||||||
Biological species | Cyanothece sp. | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||||||||
Authors | Suzuki, R. / Suzuki, E. | ||||||||||||
Funding support | Japan, 3items
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Citation | Journal: To be published Title: Structural basis for substrate binding and catalysis of branching enzyme from Cyanothece sp. ATCC 51142 Authors: Hayashi, M. / Suzuki, R. / Colleoni, C. / Ball, S.G. / Fujita, N. / Suzuki, E. #1: Journal: Acta Crystallogr F Struct Biol Commun / Year: 2015 Title: Crystallization and crystallographic analysis of branching enzymes from Cyanothece sp. ATCC 51142. Authors: Hayashi, M. / Suzuki, R. / Colleoni, C. / Ball, S.G. / Fujita, N. / Suzuki, E. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5gr3.cif.gz | 183.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5gr3.ent.gz | 140.9 KB | Display | PDB format |
PDBx/mmJSON format | 5gr3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gr/5gr3 ftp://data.pdbj.org/pub/pdb/validation_reports/gr/5gr3 | HTTPS FTP |
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-Related structure data
Related structure data | 5gqyC 5gqzC 5gr0C 5gr1C 5gr2SC 5gr4C 5gr5C 5gr6C C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 92529.211 Da / Num. of mol.: 1 / Mutation: L541A and W655A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Cyanothece sp. (strain ATCC 51142) (bacteria) Strain: ATCC 51142 / Gene: glgB, glgB1, cce_2248 / Plasmid: pET15B / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: B1WPM8, 1,4-alpha-glucan branching enzyme | ||
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#2: Chemical | ChemComp-GOL / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.67 Å3/Da / Density % sol: 73.68 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: magnesium chloride, ethanol, HEPES-NaOH / PH range: 7.2 - 7.9 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 1, 2014 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. obs: 52373 / % possible obs: 100 % / Redundancy: 14.8 % / Rmerge(I) obs: 0.082 / Net I/σ(I): 28.8 |
Reflection shell | Resolution: 2.6→2.67 Å / Redundancy: 15 % / Rmerge(I) obs: 0.371 / Mean I/σ(I) obs: 7 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5GR2 Resolution: 2.6→46.38 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.935 / SU B: 5.364 / SU ML: 0.116 / Cross valid method: THROUGHOUT / ESU R: 0.223 / ESU R Free: 0.184 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.355 Å2
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Refinement step | Cycle: LAST / Resolution: 2.6→46.38 Å
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