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- PDB-2wzj: Catalytic and UBA domain of kinase MARK2/(Par-1) K82R, T208E doub... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2wzj | ||||||
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Title | Catalytic and UBA domain of kinase MARK2/(Par-1) K82R, T208E double mutant | ||||||
![]() | SERINE/THREONINE-PROTEIN KINASE MARK2 | ||||||
![]() | TRANSFERASE / UBA DOMAIN / SERINE/THREONINE-PROTEIN KINASE / SIGNALING PROTEIN / S/T PROTEIN KINASE / DIFFERENTIATION / DEVELOPMENTAL PROTEIN | ||||||
Function / homology | ![]() establishment or maintenance of cell polarity regulating cell shape / microtubule bundle / basal cortex / regulation of microtubule binding / tau-protein kinase / establishment or maintenance of epithelial cell apical/basal polarity / regulation of postsynapse organization / establishment of cell polarity / regulation of axonogenesis / tau-protein kinase activity ...establishment or maintenance of cell polarity regulating cell shape / microtubule bundle / basal cortex / regulation of microtubule binding / tau-protein kinase / establishment or maintenance of epithelial cell apical/basal polarity / regulation of postsynapse organization / establishment of cell polarity / regulation of axonogenesis / tau-protein kinase activity / axon development / regulation of cytoskeleton organization / lateral plasma membrane / regulation of microtubule cytoskeleton organization / molecular function activator activity / actin filament / neuron migration / tau protein binding / Wnt signaling pathway / positive regulation of neuron projection development / microtubule cytoskeleton organization / postsynapse / peptidyl-serine phosphorylation / protein autophosphorylation / non-specific serine/threonine protein kinase / intracellular signal transduction / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / glutamatergic synapse / lipid binding / dendrite / magnesium ion binding / ATP binding / membrane / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Panneerselvam, S. / Marx, A. / Mandelkow, E.-M. / Mandelkow, E. | ||||||
![]() | ![]() Title: Structure and Function of Polarity-Inducing Kinase Family Mark/Par-1 within the Branch of Ampk/Snf1-Related Kinases. Authors: Marx, A. / Nugoor, C. / Panneerselvam, S. / Mandelkow, E. #1: ![]() Title: Structure of the Catalytic and Ubiquitin-Associated Domains of the Protein Kinase Mark/Par-1. Authors: Panneerselvam, S. / Marx, A. / Mandelkow, E. / Mandelkow, E. #2: ![]() Title: Structural Variations in the Catalytic and Ubiquitin-Associated Domains of Microtubule-Associated Protein/Microtubule Affinity Regulating Kinase (Mark) 1 and Mark2. Authors: Marx, A. / Nugoor, C. / Muller, J. / Panneerselvam, S. / Timm, T. / Bilang, M. / Mylonas, E. / Svergun, D.I. / Mandelkow, E. / Mandelkow, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 762.6 KB | Display | ![]() |
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PDB format | ![]() | 639.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 485.3 KB | Display | ![]() |
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Full document | ![]() | 532.7 KB | Display | |
Data in XML | ![]() | 67.2 KB | Display | |
Data in CIF | ![]() | 90.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1zmuS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37703.453 Da / Num. of mol.: 6 / Fragment: CATALYTIC AND UBA DOMAINS, RESIDUES 39-364 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: O08679, non-specific serine/threonine protein kinase #2: Water | ChemComp-HOH / | Compound details | ENGINEERED RESIDUE IN CHAIN A, LYS 82 TO ARG ENGINEERED RESIDUE IN CHAIN A, THR 208 TO GLU ...ENGINEERED | Sequence details | G38 EXPRESSION | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.36 Å3/Da / Density % sol: 63.46 % / Description: NONE |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 60% TACSIMATE PH 7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jul 28, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.78→50 Å / Num. obs: 76705 / % possible obs: 99.4 % / Observed criterion σ(I): -3 / Redundancy: 4.97 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 19.64 |
Reflection shell | Resolution: 2.78→2.95 Å / Redundancy: 4.94 % / Rmerge(I) obs: 0.69 / Mean I/σ(I) obs: 2.49 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1ZMU Resolution: 2.786→45.394 Å / SU ML: 0.43 / σ(F): 2 / Phase error: 25.11 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 50.083 Å2 / ksol: 0.329 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.786→45.394 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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