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Open data
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Basic information
Entry | Database: PDB / ID: 5gkg | ||||||||||||
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Title | Structure of EndoMS-dsDNA1'' complex | ||||||||||||
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![]() | HYDROLASE/DNA / ENDONUCLEASES / DNA-BINDING / HYDROLASE-DNA complex | ||||||||||||
Function / homology | ![]() single-stranded DNA endodeoxyribonuclease activity / Hydrolases; Acting on ester bonds / DNA binding / cytoplasm Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Nakae, S. / Hijikata, A. / Tsuji, T. / Yonezawa, K. / Kouyama, K. / Mayanagi, K. / Ishino, S. / Ishino, Y. / Shirai, T. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of the EndoMS-DNA Complex as Mismatch Restriction Endonuclease Authors: Nakae, S. / Hijikata, A. / Tsuji, T. / Yonezawa, K. / Kouyama, K.I. / Mayanagi, K. / Ishino, S. / Ishino, Y. / Shirai, T. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 143.1 KB | Display | ![]() |
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PDB format | ![]() | 108.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 464.9 KB | Display | ![]() |
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Full document | ![]() | 472.1 KB | Display | |
Data in XML | ![]() | 21.3 KB | Display | |
Data in CIF | ![]() | 29.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5gkeC ![]() 5gkfC ![]() 5gkhC ![]() 5gkiC ![]() 5gkjC ![]() 5bsl C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 28598.096 Da / Num. of mol.: 2 / Mutation: D165A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: KOD1 / Gene: TK1898 / Production host: ![]() ![]() References: UniProt: Q5JER9, Hydrolases; Acting on ester bonds |
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-DNA chain , 2 types, 2 molecules CD
#2: DNA chain | Mass: 4545.948 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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#3: DNA chain | Mass: 4675.034 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Non-polymers , 3 types, 88 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | #5: Chemical | ChemComp-MPD / ( #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.94 Å3/Da / Density % sol: 68.81 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: CALCIUM ACETATE, SODIUM CACODYLATE, PEG8000, GLYCEROL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: May 23, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→25 Å / Num. obs: 30062 / % possible obs: 91.7 % / Redundancy: 1.8 % / Net I/σ(I): 22.3 |
Reflection shell | Resolution: 2.6→2.74 Å / Redundancy: 1.8 % / Mean I/σ(I) obs: 9.4 / % possible all: 88.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5BSL ![]() 5bsl Resolution: 2.6→24.81 Å / SU ML: 0.32 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 21.04
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→24.81 Å
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Refine LS restraints |
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LS refinement shell |
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