PDB-5f7z: Crystal structure of Double Mutant S12T and N87T of Adenosine/Met...

ID or keywords:

Entry

Database: PDB / ID: 5f7z

Title

Crystal structure of Double Mutant S12T and N87T of Adenosine/Methylthioadenosine Phosphorylase from Schistosoma mansoni in APO Form

Components

Methylthioadenosine phosphorylase

Keywords

TRANSFERASE

Function / homology

Function and homology information

S-methyl-5'-thioadenosine phosphorylase / S-methyl-5-thioadenosine phosphorylase activity / L-methionine salvage from methylthioadenosine / purine ribonucleoside salvage / nucleus / cytosol
Similarity search - Function

Biological species

Schistosoma mansoni (invertebrata)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.8 Å

Authors

Torini, J.R. / Brandao-Neto, J. / DeMarco, R. / Pereira, H.M.

Funding support

Brazil, 2items

Citation

Journal: PLoS Negl Trop Dis / Year: 2016
Title: Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5'-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway.
Authors: Torini, J.R. / Brandao-Neto, J. / DeMarco, R. / Pereira, H.D.

History

Deposition	Dec 8, 2015	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Dec 21, 2016	Provider: repository / Type: Initial release
Revision 1.1	Sep 27, 2017	Group: Author supporting evidence / Data collection / Category: diffrn_source / pdbx_audit_support Item: _diffrn_source.pdbx_synchrotron_site / _pdbx_audit_support.funding_organization
Revision 1.2	Nov 1, 2017	Group: Author supporting evidence / Category: pdbx_struct_assembly_auth_evidence
Revision 1.3	Apr 17, 2019	Group: Author supporting evidence / Data collection / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4	Jan 1, 2020	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.5	Sep 27, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5f7z.cif.gz	680.9 KB	Display	PDBx/mmCIF format
PDB format	pdb5f7z.ent.gz	559.9 KB	Display	PDB format
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Validation report

Summary document	5f7z_validation.pdf.gz	495 KB	Display	wwPDB validaton report
Full document	5f7z_full_validation.pdf.gz	505.1 KB	Display
Data in XML	5f7z_validation.xml.gz	74 KB	Display
Data in CIF	5f7z_validation.cif.gz	106.3 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/f7/5f7z ftp://data.pdbj.org/pub/pdb/validation_reports/f7/5f7z	HTTPS FTP

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Related structure data

Related structure data	4l5aC 4l5cC 4l5yC 4l6iC 5f73C 5f76C 5f77C 5f78SC 5f7jC 5f7oC 5f7xC 5fakC C: citing same article (ref.) S: Starting model for refinement
Similar structure data	4l5y-2 5f78-2 5f78-1 5f7o-d 5f77-d 4l5y-1 5fak-2 5f73-1 5fak-1 5f76-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: Methylthioadenosine phosphorylase

B: Methylthioadenosine phosphorylase

C: Methylthioadenosine phosphorylase

D: Methylthioadenosine phosphorylase

E: Methylthioadenosine phosphorylase

F: Methylthioadenosine phosphorylase

hetero molecules

212 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Methylthioadenosine phosphorylase

C: Methylthioadenosine phosphorylase

F: Methylthioadenosine phosphorylase

hetero molecules

defined by author&software
Evidence: gel filtration
106 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Methylthioadenosine phosphorylase

D: Methylthioadenosine phosphorylase

E: Methylthioadenosine phosphorylase

hetero molecules

defined by author&software
Evidence: gel filtration
106 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	80.701, 82.255, 150.018
Angle α, β, γ (deg.)	90.000, 101.420, 90.000
Int Tables number	4
Space group name H-M	P12₁1

Details

Trimer confirmed by gel filtration

#1: Protein	Methylthioadenosine phosphorylase Mass: 35260.508 Da / Num. of mol.: 6 / Fragment: Enzyme / Mutation: S12T,N87T Source method: isolated from a genetically manipulated source Source: (gene. exp.) Schistosoma mansoni (invertebrata) / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: I0B503, S-methyl-5'-thioadenosine phosphorylase
#2: Chemical	ChemComp-PO4 / PHOSPHATE ION Mass: 94.971 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: PO₄
#3: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1493 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.31 Å³/Da / Density % sol: 46.68 %
Crystal grow	Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 / Details: 100mM Bris-tris or Mes, 14-18% PEG3350, pH 6.5 / PH range: 6.1-6.7

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

Diamond

/ Beamline: I04 / Wavelength: 0.97949 Å

Detector

Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 21, 2015

Radiation

Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.97949 Å / Relative weight: 1

Reflection

Resolution: 1.8→82.26 Å / Num. obs: 178037 / % possible obs: 99.9 % / Redundancy: 3.3 % / R_merge(I) obs: 0.086 / Net I/σ(I): 7.1 / Scaling rejects: 53

Reflection shell

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Diffraction-ID	Rejects	% possible all
1.8-1.94	3.3	0.67	1	0	99.9
4.76-82.26	3.3	0.054	1	0	100

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Processing

Software

Name	Version	Classification
xia2		data scaling
PHASER		phasing
PHENIX		refinement
PDB_EXTRACT	3.15	data extraction
xia2		data reduction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 5F78

5f78

Resolution: 1.8→79.103 Å / SU ML: 0.27 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 28.1 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.2354	8741	4.94 %	Random selection
R_work	0.2107	168358	-	-
obs	0.2119	177099	99.43 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 93.66 Å² / B_iso mean: 32.2947 Å² / B_iso min: 11.65 Å²

Refinement step

Cycle: final / Resolution: 1.8→79.103 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	12804	0	30	1493	14327
B_iso mean	-	-	20.04	38.5	-
Num. residues	-	-	-	-	1703

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	13074
X-RAY DIFFRACTION	f_angle_d	0.532	17765
X-RAY DIFFRACTION	f_chiral_restr	0.044	2084
X-RAY DIFFRACTION	f_plane_restr	0.004	2278
X-RAY DIFFRACTION	f_dihedral_angle_d	10.918	7817

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
1.8-1.8205	0.4204	275	0.3885	5436	5711	98
1.8205-1.8419	0.3844	330	0.3827	5454	5784	98
1.8419-1.8643	0.3871	284	0.3618	5515	5799	98
1.8643-1.8879	0.391	294	0.366	5522	5816	98
1.8879-1.9128	0.3726	273	0.3395	5559	5832	99
1.9128-1.939	0.3629	264	0.3214	5542	5806	99
1.939-1.9667	0.3047	262	0.3108	5643	5905	99
1.9667-1.996	0.3479	327	0.3041	5537	5864	99
1.996-2.0272	0.3278	287	0.2865	5613	5900	100
2.0272-2.0605	0.2963	296	0.2823	5661	5957	100
2.0605-2.096	0.299	304	0.267	5558	5862	100
2.096-2.1341	0.2961	295	0.2638	5619	5914	100
2.1341-2.1752	0.3165	297	0.2555	5585	5882	100
2.1752-2.2196	0.2723	276	0.2352	5661	5937	100
2.2196-2.2678	0.2483	262	0.23	5589	5851	100
2.2678-2.3206	0.2412	283	0.2233	5653	5936	100
2.3206-2.3786	0.2677	276	0.2144	5639	5915	100
2.3786-2.443	0.2361	274	0.2113	5643	5917	100
2.443-2.5149	0.2416	297	0.2075	5641	5938	100
2.5149-2.596	0.239	260	0.2082	5627	5887	100
2.596-2.6888	0.2348	300	0.1946	5633	5933	100
2.6888-2.7965	0.2175	321	0.1976	5613	5934	100
2.7965-2.9238	0.2262	284	0.1944	5647	5931	100
2.9238-3.0779	0.2326	342	0.1989	5588	5930	100
3.0779-3.2708	0.2096	314	0.192	5656	5970	100
3.2708-3.5233	0.2112	308	0.1833	5664	5972	100
3.5233-3.8778	0.1961	280	0.1641	5677	5957	100
3.8778-4.4389	0.157	305	0.1473	5677	5982	100
4.4389-5.5924	0.16	282	0.1523	5709	5991	100
5.5924-79.179	0.1926	289	0.1799	5797	6086	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.7826	-0.9605	-1.1459	2.7507	1.5993	2.2696	-0.4471	0.5685	-0.6331	0.1412	0.1163	0.3583	0.5903	-0.2932	0.3277	0.2765	-0.0302	0.0359	0.3103	-0.102	0.3325	94.5452	-18.5071	31.0457
2	1.532	-0.4408	-0.3669	1.1131	0.4298	1.9681	-0.0573	0.1224	-0.3293	0.0613	-0.054	0.0912	0.3269	-0.0311	0.0643	0.2436	0.0081	0.0353	0.1896	-0.044	0.2502	96.4991	-16.6292	39.1376
3	1.9241	-0.069	-0.2998	1.1301	0.2342	1.5188	0.0661	0.1828	0.013	0.0235	-0.0317	0.1566	-0.284	-0.1763	-0.0501	0.251	0.0274	0.0211	0.22	-0.0269	0.1922	91.6807	0.553	41.3439
4	1.328	-0.9835	0.8735	1.4536	0.489	1.8087	-0.0202	0.0567	-0.1077	0.1235	-0.0069	0.0018	0.2861	0.0743	-0.0537	0.2238	-0.0121	0.0489	0.1751	0.0035	0.1749	106.4098	-4.7224	48.6441
5	1.9559	-0.1979	-0.0657	0.8378	0.2655	1.812	0.3009	0.5146	0.3391	-0.3405	-0.1734	0.0059	-0.6097	-0.2333	-0.0633	0.3897	0.121	0.0329	0.3275	0.0308	0.2345	92.6982	4.538	25.8349
6	1.3084	-0.7169	-0.7191	0.4908	0.4781	0.9054	0.0899	0.1682	-0.0017	-0.0182	-0.1269	0.0866	-0.2071	-0.1618	-0.0162	0.2341	0.029	0.0346	0.1852	-0.0262	0.1891	89.1921	1.3377	44.4199
7	0.0707	0.152	-0.1032	0.3833	-0.3724	0.3155	-0.1079	-0.4295	-0.3578	0.1693	0.1368	0.4151	-0.117	-0.3525	0.1123	0.2444	0.0997	0.0115	0.4114	-0.0321	0.3526	82.0615	-2.3052	35.3302
8	6.5666	0.5508	-0.4423	0.9836	-0.2727	1.0218	-0.0659	0.3961	-0.1779	-0.232	-0.1041	0.2152	0.1205	-0.5231	0.0651	0.2877	0.0216	-0.0286	0.461	-0.1365	0.2454	85.8057	-8.168	25.599
9	1.1468	0.1325	0.145	0.5322	0.4152	5.2738	-0.0135	0.123	-0.0616	-0.0455	0.0974	-0.1773	-0.0304	1.1608	-0.0606	0.2801	0.0594	0.0634	0.4099	-0.0016	0.3059	114.1431	-5.4636	38.3729
10	0.3463	0.6373	-0.8055	2.0345	-1.3111	1.5671	-0.3328	-0.4578	-1.1163	-0.0063	-0.2877	-0.4246	0.4486	0.341	0.5447	0.3692	-0.0498	0.1004	0.4245	0.1504	0.4526	133.1267	21.4696	43.62
11	1.2725	0.3157	-0.4727	1.2875	-0.7324	1.0955	0.0995	-0.2445	-0.0891	0.2395	-0.1344	0.0762	-0.0852	0.0446	0.054	0.2478	-0.0527	0.0391	0.2235	0.0213	0.1877	134.5811	35.878	36.3265
12	0.1296	-0.2891	0.4625	1.2203	-0.1953	1.6939	-0.1142	-0.002	0.0981	0.1719	-0.1215	-0.1521	-0.2374	0.4192	0.1011	0.2726	-0.1047	-0.0012	0.3782	0.0922	0.226	140.035	36.5754	36.3594
13	0.264	0.2974	-0.7466	1.3662	-0.2435	2.556	-0.0043	-0.3447	-0.0476	0.3701	-0.1978	0.083	0.0298	0.0006	0.1616	0.3698	-0.0983	0.0659	0.3218	0.0633	0.2558	130.6937	33.3594	43.2422
14	1.6415	0.9101	0.5567	3.3372	-0.3416	1.9152	-0.0306	-0.2539	-0.1594	0.5662	-0.1177	0.4576	0.3118	-0.4444	0.1178	0.3374	-0.0649	0.1612	0.3063	0.0119	0.4738	67.6263	-7.1428	72.3778
15	1.6883	-0.2123	-0.3721	1.9667	-0.4066	2.0056	-0.057	0.0825	-0.3068	0.0669	-0.0023	0.2006	0.1102	-0.2402	0.0376	0.1851	-0.0024	0.0596	0.1631	0.0121	0.2853	73.1721	-0.1353	65.1209
16	1.8156	-0.2184	-0.8317	1.5526	0.4272	2.1364	-0.1296	0.1445	-0.3489	0.0124	-0.0597	0.5592	-0.0488	-0.2509	0.1353	0.1978	0.0157	0.0278	0.2603	0.0168	0.3169	67.4652	5.447	56.36
17	1.3408	0.3004	-0.0368	0.5041	0.0831	1.303	0.0204	0.1292	0.0055	-0.0045	0.0499	0.0741	-0.1531	-0.0713	-0.0741	0.2264	0.0311	0.0518	0.1452	0.021	0.2356	78.5781	10.7457	60.8763
18	0.5127	-0.6494	-0.7112	0.7277	0.6301	1.6741	-0.1092	-0.1678	-0.2075	-0.4431	0.4899	-0.7153	-0.6998	0.2359	-0.4005	0.2937	0.0026	0.0644	0.2444	-0.0478	0.4262	77.1553	12.7431	69.763
19	4.9118	1.6842	2.3527	1.9642	1.3634	3.3257	-0.1573	-1.0535	0.2276	0.099	-0.12	0.3524	-0.4151	-0.6235	0.2302	0.4004	0.0874	0.1608	0.3976	0.0008	0.4065	66.4773	11.4466	74.3296
20	2.0322	-0.2477	-0.1737	1.3815	-0.7562	1.8237	-0.2121	0.7985	-0.595	-0.1402	-0.0599	0.4834	0.7407	-0.8195	0.0207	0.4043	-0.1411	-0.0597	0.5503	-0.1076	0.652	63.6564	-8.0533	49.1297
21	4.3211	-3.9491	-0.3422	4.2559	0.3927	0.5206	0.2236	0.908	-0.5036	-0.9301	-0.3788	-0.6467	0.477	0.4734	0.0526	0.2909	0.0698	0.0952	0.3339	-0.0267	0.4629	162.7589	36.5001	0.5468
22	1.2981	0.2735	-0.1353	2.253	0.6452	1.6372	-0.0216	0.0077	-0.2124	-0.0716	-0.0449	-0.2384	0.1121	0.1911	0.0604	0.11	0.0075	0.0036	0.1379	0.0102	0.1989	155.415	37.2238	6.5268
23	1.3254	0.14	-0.3191	0.8403	-0.096	1.326	0.0261	-0.1455	-0.0282	0.0692	-0.0836	-0.1387	-0.0411	0.1506	0.0787	0.1272	-0.0217	-0.0345	0.1696	0.0056	0.1725	153.8469	45.8039	15.1848
24	2.8601	0.8105	-0.6094	1.6892	-0.2574	1.7905	0.0186	0.0154	-0.1046	0.055	-0.0474	-0.2783	-0.0553	0.284	-0.0024	0.1328	-0.0176	-0.0162	0.1733	-0.0269	0.2311	160.583	42.6074	9.7633
25	0.6658	0.0391	1.502	0.7296	0.8179	4.2854	-0.059	0.5674	-0.0856	-0.2379	-0.0171	0.8163	0.7191	-1.1925	0.0011	0.2632	-0.0021	-0.0855	0.602	-0.1022	0.3627	110.3649	47.4476	2.1867
26	0.9046	0.5498	-0.4727	1.2918	0.3753	0.75	-0.0139	1.1276	-0.3568	-0.3143	-0.311	0.7844	0.0722	-0.8739	0.234	0.3042	-0.0394	-0.0571	0.5629	-0.1928	0.4415	116.3393	34.5191	-0.4953
27	1.7211	-0.4136	-0.7152	1.6008	0.5118	0.9364	0.1286	0.2062	-0.006	-0.0834	-0.1461	0.0985	-0.1158	-0.2577	-0.0049	0.1646	0.051	-0.0374	0.2249	-0.0156	0.1533	122.2418	52.0314	7.9386
28	1.481	0.0302	-0.5547	1.7372	-0.1236	1.6837	-0.05	-0.1804	0.0458	0.2296	-0.122	0.2239	-0.0789	-0.1688	0.0532	0.2236	0.0132	-0.0082	0.1953	0.0033	0.183	129.0547	49.0207	27.2439
29	2.3172	-0.3281	-0.2332	1.6328	0.1999	0.526	0.063	0.0526	0.0101	0.1307	-0.0597	0.118	-0.1581	-0.1762	-0.0252	0.1823	0.0377	-0.0095	0.1595	-0.0154	0.1642	123.6834	50.9383	12.4785
30	1.941	-0.6314	-0.4475	1.6031	0.0475	0.4096	0.2609	0.316	0.1849	-0.2089	-0.1871	0.1148	-0.2204	-0.2086	-0.0833	0.2549	0.0798	-0.0654	0.2848	-0.0441	0.1903	118.8782	54.3859	2.2147
31	1.6922	0.351	2.4764	0.8918	-0.6916	5.3248	0.1441	-0.1376	-0.1774	0.1473	-0.0135	-0.3969	0.3713	0.6352	-0.0323	0.2768	-0.0142	-0.0048	0.2669	0.0119	0.2438	117.8723	5.9206	72.6685
32	3.9426	0.2807	-2.0189	5.5357	-0.8784	2.6805	-0.196	-0.1394	-0.1942	0.1986	0.0269	-0.4715	0.0633	0.5596	0.1943	0.242	0.048	-0.0181	0.3046	0.0443	0.2436	110.6888	-6.5314	75.5707
33	1.5353	-0.0634	0.1447	1.6168	-0.2243	1.2442	0.0192	-0.198	-0.0771	0.2346	0.0226	-0.0562	-0.0492	0.2242	-0.0084	0.2372	-0.0192	0.0054	0.2141	0.0204	0.1746	110.6606	2.5883	71.8157
34	1.5782	-0.0855	-0.2731	1.1708	-0.0043	0.974	0.0624	-0.0126	-0.0266	0.0322	0.0237	0.1499	-0.0863	-0.0321	-0.0724	0.2315	-0.0156	0.0043	0.1504	-0.0115	0.2014	98.0863	8.5417	65.5949
35	0.706	-0.0546	-0.012	1.6178	0.3113	0.9953	0.0831	0.0218	0.128	0.0119	-0.031	-0.0218	-0.2222	0.0577	-0.0348	0.2537	-0.0344	0.0501	0.1687	0.0013	0.2131	106.7173	19.944	59.1072
36	1.5382	0.0787	-0.0829	1.3972	-0.1998	0.4703	0.0698	0.0623	-0.1048	-0.0371	-0.0281	0.0183	-0.1813	0.0835	-0.03	0.2507	-0.0223	0.0201	0.1504	-0.018	0.1717	104.4496	10.1957	62.6165
37	1.6151	-0.0558	0.2131	2.0365	0.4773	1.1218	0.0466	-0.2849	0.1106	0.2985	0.0126	-0.0411	-0.1778	0.1575	-0.0504	0.3347	-0.0423	-0.0204	0.168	0.0181	0.1643	109.1478	13.5535	73.0714

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 3 through 26 )	A
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 27 through 89 )	A
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 90 through 120 )	A
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 121 through 139 )	A
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 140 through 182 )	A
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 183 through 220 )	A
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 221 through 240 )	A
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 241 through 269 )	A
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 270 through 291 )	A
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 3 through 26 )	B
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 27 through 210 )	B
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 211 through 238 )	B
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 239 through 291 )	B
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 3 through 38 )	C
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 39 through 120 )	C
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 121 through 164 )	C
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 165 through 220 )	C
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 221 through 238 )	C
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 239 through 269 )	C
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 270 through 291 )	C
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 3 through 23 )	D
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 24 through 110 )	D
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 111 through 220 )	D
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 221 through 291 )	D
25	X-RAY DIFFRACTION	25	chain 'E' and (resid 3 through 23 )	E
26	X-RAY DIFFRACTION	26	chain 'E' and (resid 24 through 38 )	E
27	X-RAY DIFFRACTION	27	chain 'E' and (resid 39 through 182 )	E
28	X-RAY DIFFRACTION	28	chain 'E' and (resid 183 through 198 )	E
29	X-RAY DIFFRACTION	29	chain 'E' and (resid 199 through 242 )	E
30	X-RAY DIFFRACTION	30	chain 'E' and (resid 243 through 291 )	E
31	X-RAY DIFFRACTION	31	chain 'F' and (resid 3 through 23 )	F
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 24 through 38 )	F
33	X-RAY DIFFRACTION	33	chain 'F' and (resid 39 through 90 )	F
34	X-RAY DIFFRACTION	34	chain 'F' and (resid 91 through 139 )	F
35	X-RAY DIFFRACTION	35	chain 'F' and (resid 140 through 198 )	F
36	X-RAY DIFFRACTION	36	chain 'F' and (resid 199 through 242 )	F
37	X-RAY DIFFRACTION	37	chain 'F' and (resid 243 through 291 )	F

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