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- PDB-5eqh: Human GLUT1 in complex with inhibitor (2~{S})-3-(2-bromophenyl)-2... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5eqh | ||||||||||||
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Title | Human GLUT1 in complex with inhibitor (2~{S})-3-(2-bromophenyl)-2-[2-(4-methoxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide | ||||||||||||
![]() | Solute carrier family 2, facilitated glucose transporter member 1 | ||||||||||||
![]() | TRANSPORT PROTEIN/INHIBITOR / MFS transporter / glucose transporter / TRANSPORT PROTEIN-INHIBITOR complex | ||||||||||||
Function / homology | ![]() glucose transporter complex / Defective SLC2A1 causes GLUT1 deficiency syndrome 1 (GLUT1DS1) / long-chain fatty acid import across plasma membrane / response to Thyroglobulin triiodothyronine / dehydroascorbic acid transmembrane transporter activity / dehydroascorbic acid transport / Lactose synthesis / D-glucose transmembrane transporter activity / Cellular hexose transport / glucose transmembrane transporter activity ...glucose transporter complex / Defective SLC2A1 causes GLUT1 deficiency syndrome 1 (GLUT1DS1) / long-chain fatty acid import across plasma membrane / response to Thyroglobulin triiodothyronine / dehydroascorbic acid transmembrane transporter activity / dehydroascorbic acid transport / Lactose synthesis / D-glucose transmembrane transporter activity / Cellular hexose transport / glucose transmembrane transporter activity / glucose import across plasma membrane / Vitamin C (ascorbate) metabolism / L-ascorbic acid metabolic process / glucose transmembrane transport / photoreceptor cell maintenance / cellular hyperosmotic response / female germ cell nucleus / female pronucleus / long-chain fatty acid transmembrane transporter activity / cortical actin cytoskeleton / glucose import / xenobiotic transmembrane transporter activity / transport across blood-brain barrier / intercalated disc / cellular response to glucose starvation / photoreceptor inner segment / caveola / central nervous system development / Regulation of insulin secretion / female pregnancy / response to insulin / cerebral cortex development / sarcolemma / kinase binding / Z disc / cellular response to mechanical stimulus / : / melanosome / presynapse / midbody / protein-containing complex assembly / basolateral plasma membrane / blood microparticle / response to hypoxia / apical plasma membrane / Golgi membrane / extracellular exosome / identical protein binding / membrane / plasma membrane / cytosol Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Kapoor, K. / Finer-Moore, J. / Pedersen, B.P. / Caboni, L. / Waight, A.B. / Hillig, R. / Bringmann, P. / Heisler, I. / Muller, T. / Siebeneicher, H. / Stroud, R.M. | ||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Mechanism of inhibition of human glucose transporter GLUT1 is conserved between cytochalasin B and phenylalanine amides. Authors: Kapoor, K. / Finer-Moore, J.S. / Pedersen, B.P. / Caboni, L. / Waight, A. / Hillig, R.C. / Bringmann, P. / Heisler, I. / Muller, T. / Siebeneicher, H. / Stroud, R.M. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 177.5 KB | Display | ![]() |
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PDB format | ![]() | 141.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 718.5 KB | Display | ![]() |
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Full document | ![]() | 722.5 KB | Display | |
Data in XML | ![]() | 17 KB | Display | |
Data in CIF | ![]() | 22.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5eqgC ![]() 5eqiC ![]() 4pypS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54125.395 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-5RF / ( |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.9 Å3/Da / Density % sol: 68.46 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 30-40% v/v PEG400, 100 mM MES, pH 5.5-6.5, 50-200 mM sodium chloride or magnesium chloride PH range: 5.5-6.5 |
-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 30, 2015 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.909 Å / Relative weight: 1 |
Reflection | Resolution: 2.992→50 Å / Num. obs: 14959 / % possible obs: 89.1 % / Observed criterion σ(I): -3 / Redundancy: 7 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 3→3.05 Å / Redundancy: 2.9 % / Mean I/σ(I) obs: 0.53 / % possible all: 39.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 4PYP Resolution: 2.99→41.84 Å / SU ML: 0.46 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 31.52 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.99→41.84 Å
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Refine LS restraints |
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LS refinement shell |
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