- PDB-5epo: The three-dimensional structure of Clostridium absonum 7alpha-hyd... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 5epo
Title
The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase
Components
7-alpha-hydroxysteroid deydrogenase
Keywords
OXIDOREDUCTASE / Oxidoreductase Activity / Metabolic Process
Function / homology
Function and homology information
bile acid catabolic process / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / lipid catabolic process / oxidoreductase activity / nucleotide binding Similarity search - Function
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.5419 Å / Relative weight: 1
Reflection
Resolution: 1.726→110.195 Å / Num. obs: 69768 / % possible obs: 98.3 % / Redundancy: 6.7 % / Net I/σ(I): 7.1
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0135
refinement
SCALA
datascaling
PHASER
phasing
Refinement
Resolution: 2→49.58 Å / Cor.coef. Fo:Fc: 0.934 / Cor.coef. Fo:Fc free: 0.893 / SU B: 5.008 / SU ML: 0.133 / Cross valid method: THROUGHOUT / ESU R: 0.211 / ESU R Free: 0.17 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22189
3540
5.1 %
RANDOM
Rwork
0.1821
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obs
0.18411
66141
98.22 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK