development of primary sexual characteristics / PRC1 complex / PcG protein complex / SUMOylation of DNA methylation proteins / SUMOylation of RNA binding proteins / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / heterochromatin / SUMOylation of DNA damage response and repair proteins / methylated histone binding / SUMOylation of chromatin organization proteins ...development of primary sexual characteristics / PRC1 complex / PcG protein complex / SUMOylation of DNA methylation proteins / SUMOylation of RNA binding proteins / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / heterochromatin / SUMOylation of DNA damage response and repair proteins / methylated histone binding / SUMOylation of chromatin organization proteins / SUMOylation of transcription cofactors / Regulation of PTEN gene transcription / euchromatin / chromatin organization / Oxidative Stress Induced Senescence / cell differentiation / chromatin binding / negative regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus 類似検索 - 分子機能
Chromobox protein homolog 2 / CBX family C-terminal motif / CBX family C-terminal motif / Chromo domain, conserved site / Chromo domain signature. / Chromo domain / Chromo (CHRromatin Organisation MOdifier) domain / Chromo and chromo shadow domain profile. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #40 / Chromo/chromo shadow domain ...Chromobox protein homolog 2 / CBX family C-terminal motif / CBX family C-terminal motif / Chromo domain, conserved site / Chromo domain signature. / Chromo domain / Chromo (CHRromatin Organisation MOdifier) domain / Chromo and chromo shadow domain profile. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #40 / Chromo/chromo shadow domain / Chromatin organization modifier domain / Chromo-like domain superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta 類似検索 - ドメイン・相同性
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
解像度: 1.8→34.16 Å / Num. obs: 10262 / % possible obs: 100 % / 冗長度: 10.6 % / Rmerge(I) obs: 0.067 / Net I/σ(I): 29.2
反射 シェル
解像度: 1.8→1.84 Å / 冗長度: 10.3 % / Rmerge(I) obs: 0.886 / Mean I/σ(I) obs: 3.1 / % possible all: 100
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.8.0123
精密化
Aimless
0.5.1
データスケーリング
PDB_EXTRACT
3.2
データ抽出
PHASER
位相決定
精密化
解像度: 1.8→34 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.945 / SU B: 3.69 / SU ML: 0.066 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.096 / ESU R Free: 0.094 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: PHENIX.ELBOW/MOGUL WAS USED TO GENERATE GEOMETRY RESTRAINTS FOR INHIBITOR BUILDING BLOCKS. JLIGAND WAS USED FOR PREPARATION OF LINK RESTRAINTS. LINK RESTRAINTS WERE MANUALLY MODIFIED, FOR ...詳細: PHENIX.ELBOW/MOGUL WAS USED TO GENERATE GEOMETRY RESTRAINTS FOR INHIBITOR BUILDING BLOCKS. JLIGAND WAS USED FOR PREPARATION OF LINK RESTRAINTS. LINK RESTRAINTS WERE MANUALLY MODIFIED, FOR EXAMPLE TO ESTABLISH PLANAR GEOMETRY OF METHYL ESTER TERMINUS OF INHIBITOR. COOT WAS USED FOR INTERACTIVE MODEL BUILDING. MODEL GEOMETRY WAS EVALUATED WITH MOLPROBITY. ELECTRON DENSITY DOES NOT FULLY RESOLVE THE LIGAND'S N-EPSILON ETHYLATION AND C-TERMINAL SERINE METHYL ESTER MOIETY.
Rfactor
反射数
%反射
Rfree
0.212
506
4.9 %
Rwork
0.189
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-
obs
0.19
9752
100 %
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK