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- PDB-5ejk: Crystal structure of the Rous sarcoma virus intasome -

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Basic information

Entry
Database: PDB / ID: 5ejk
TitleCrystal structure of the Rous sarcoma virus intasome
Components
  • DNA (5'-D(*AP*AP*TP*GP*TP*TP*GP*TP*CP*TP*TP*AP*TP*GP*CP*AP*AP*TP*AP*CP*TP*C)-3')
  • DNA (5'-D(*AP*GP*TP*GP*TP*CP*TP*T)-3')
  • DNA (5'-D(*CP*TP*TP*CP*TP*CP*TP*C)-3')
  • Gag-Pro-Pol polyprotein
  • RSV Integrase
KeywordsTransferase/DNA / RSV / integrase / intasome / Transferase-DNA complex
Function / homology
Function and homology information


Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / RNA stem-loop binding / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases ...Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / RNA stem-loop binding / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / Hydrolases; Acting on ester bonds / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / proteolysis / DNA binding / zinc ion binding
Similarity search - Function
Retroviral Gag polyprotein, M / Retroviral M domain / gag protein p24 N-terminal domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral ...Retroviral Gag polyprotein, M / Retroviral M domain / gag protein p24 N-terminal domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain / Integrase, C-terminal, retroviral / Integrase DNA binding domain profile. / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Retropepsin-like catalytic domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Ribonuclease H domain / RNase H type-1 domain profile. / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase domain / Reverse transcriptase (RT) catalytic domain profile. / Retropepsins / Retroviral aspartyl protease / Aspartyl protease, retroviral-type family profile. / Peptidase A2A, retrovirus, catalytic / Retrovirus capsid, C-terminal / Retroviral matrix protein / Retrovirus capsid, N-terminal / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Ribonuclease H superfamily / Aspartic peptidase domain superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
: / DNA / DNA (> 10) / Gag-Pol polyprotein
Similarity search - Component
Biological speciesRous sarcoma virus
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.8 Å
AuthorsYin, Z. / Shi, K. / Banerjee, S. / Aihara, H.
CitationJournal: Nature / Year: 2016
Title: Crystal structure of the Rous sarcoma virus intasome.
Authors: Yin, Z. / Shi, K. / Banerjee, S. / Pandey, K.K. / Bera, S. / Grandgenett, D.P. / Aihara, H.
History
DepositionNov 2, 2015Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 17, 2016Provider: repository / Type: Initial release
Revision 1.1Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_oper_list / struct_conn / struct_conn_type
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_oper_list.symmetry_operation / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry / _struct_conn_type.id
Revision 1.2Nov 15, 2023Group: Data collection / Category: chem_comp_atom / chem_comp_bond / Item: _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_2

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Gag-Pro-Pol polyprotein
B: Gag-Pro-Pol polyprotein
C: Gag-Pro-Pol polyprotein
D: Gag-Pro-Pol polyprotein
E: Gag-Pro-Pol polyprotein
F: Gag-Pro-Pol polyprotein
G: Gag-Pro-Pol polyprotein
H: Gag-Pro-Pol polyprotein
I: RSV Integrase
J: DNA (5'-D(*AP*AP*TP*GP*TP*TP*GP*TP*CP*TP*TP*AP*TP*GP*CP*AP*AP*TP*AP*CP*TP*C)-3')
K: DNA (5'-D(*CP*TP*TP*CP*TP*CP*TP*C)-3')
L: RSV Integrase
M: DNA (5'-D(*AP*AP*TP*GP*TP*TP*GP*TP*CP*TP*TP*AP*TP*GP*CP*AP*AP*TP*AP*CP*TP*C)-3')
N: DNA (5'-D(*CP*TP*TP*CP*TP*CP*TP*C)-3')
k: DNA (5'-D(*AP*GP*TP*GP*TP*CP*TP*T)-3')
n: DNA (5'-D(*AP*GP*TP*GP*TP*CP*TP*T)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)300,81760
Polymers293,67616
Non-polymers7,14244
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area46770 Å2
ΔGint-487 kcal/mol
Surface area115740 Å2
MethodPISA
Unit cell
Length a, b, c (Å)124.936, 157.854, 126.583
Angle α, β, γ (deg.)90.00, 110.94, 90.00
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 1 types, 8 molecules ABCDEFGH

#1: Protein
Gag-Pro-Pol polyprotein


Mass: 30574.482 Da / Num. of mol.: 8 / Fragment: UNP residues 573-842 / Mutation: C23S, L112M, L135M, L162M, L163M, L188 M, L189M
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rous sarcoma virus (strain Prague C) / Strain: Prague C / Gene: gag-pro-pol / Production host: Escherichia coli (E. coli)
References: UniProt: P03354, Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases, RNA-directed DNA polymerase, DNA-directed DNA polymerase, ribonuclease H, Transferases; ...References: UniProt: P03354, Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases, RNA-directed DNA polymerase, DNA-directed DNA polymerase, ribonuclease H, Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases, Hydrolases; Acting on ester bonds

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DNA chain , 4 types, 8 molecules ILJMKNkn

#2: DNA chain RSV Integrase


Mass: 13047.456 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rous sarcoma virus (strain Schmidt-Ruppin E)
Production host: Escherichia coli (E. coli)
#3: DNA chain DNA (5'-D(*AP*AP*TP*GP*TP*TP*GP*TP*CP*TP*TP*AP*TP*GP*CP*AP*AP*TP*AP*CP*TP*C)-3')


Mass: 6716.363 Da / Num. of mol.: 2 / Source method: obtained synthetically
Source: (synth.) Rous sarcoma virus (strain Schmidt-Ruppin E)
#4: DNA chain DNA (5'-D(*CP*TP*TP*CP*TP*CP*TP*C)-3')


Mass: 2343.552 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#5: DNA chain DNA (5'-D(*AP*GP*TP*GP*TP*CP*TP*T)-3')


Mass: 2432.614 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)

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Non-polymers , 2 types, 44 molecules

#6: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Zn
#7: Chemical...
ChemComp-W / TUNGSTEN ION


Mass: 183.840 Da / Num. of mol.: 36 / Source method: obtained synthetically / Formula: W

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Details

Nonpolymer detailsThe 12-POLYTUNGSTATE cluster (W) represent the ligand E43 (H2 O40 W12) and the oxygens in these 3 ...The 12-POLYTUNGSTATE cluster (W) represent the ligand E43 (H2 O40 W12) and the oxygens in these 3 clusters have not been modeled in this structure

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 4.12 Å3/Da / Density % sol: 70.16 %
Crystal growTemperature: 293 K / Method: vapor diffusion / Details: Sodium formate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 24, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 3.61→49.248 Å / Num. obs: 52483 / % possible obs: 99 % / Redundancy: 13.9 % / Net I/σ(I): 9.6
Reflection shellRmerge(I) obs: 0.147

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Processing

Software
NameVersionClassification
PHENIX(dev_2210: ???)refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4FW1, 1K6Y

4fw1

1k6y


Resolution: 3.8→49.215 Å / SU ML: 0.84 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 37.96 / Stereochemistry target values: ML
Details: The data were processed to 3.6A, and the refinement is done upto 3.8A
RfactorNum. reflection% reflection
Rfree0.2945 2208 4.87 %
Rwork0.2537 --
obs0.2557 37364 96.96 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.8→49.215 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16212 3097 44 0 19353
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00820106
X-RAY DIFFRACTIONf_angle_d0.71527815
X-RAY DIFFRACTIONf_dihedral_angle_d17.64211530
X-RAY DIFFRACTIONf_chiral_restr0.0463058
X-RAY DIFFRACTIONf_plane_restr0.0123076
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.8-3.84320.5617980.47861796X-RAY DIFFRACTION64
3.8432-3.88840.48341070.42752510X-RAY DIFFRACTION89
3.8884-3.93580.4711340.41312783X-RAY DIFFRACTION97
3.9358-3.98560.42241220.4032756X-RAY DIFFRACTION98
3.9856-4.0380.3811600.39992809X-RAY DIFFRACTION99
4.038-4.09330.34451650.38352759X-RAY DIFFRACTION99
4.0933-4.15170.40991100.39282828X-RAY DIFFRACTION99
4.1517-4.21370.38281340.35622804X-RAY DIFFRACTION99
4.2137-4.27950.38411430.35232808X-RAY DIFFRACTION99
4.2795-4.34960.39261340.35352775X-RAY DIFFRACTION99
4.3496-4.42450.3571800.3522753X-RAY DIFFRACTION99
4.4245-4.50490.37441800.34642797X-RAY DIFFRACTION99
4.5049-4.59150.40381430.32662773X-RAY DIFFRACTION99
4.5915-4.68520.36451570.31922758X-RAY DIFFRACTION98
4.6852-4.7870.33851590.30272748X-RAY DIFFRACTION98
4.787-4.89820.3621400.30742736X-RAY DIFFRACTION98
4.8982-5.02060.32361280.29042704X-RAY DIFFRACTION96
5.0206-5.15620.35521840.31182766X-RAY DIFFRACTION99
5.1562-5.30780.33981210.30922850X-RAY DIFFRACTION99
5.3078-5.47890.38881670.31072799X-RAY DIFFRACTION99
5.4789-5.67440.34821170.29332779X-RAY DIFFRACTION100
5.6744-5.90130.3181000.28752863X-RAY DIFFRACTION99
5.9013-6.16930.3121440.31382783X-RAY DIFFRACTION99
6.1693-6.49390.34021570.29322796X-RAY DIFFRACTION99
6.4939-6.89980.34591230.27352791X-RAY DIFFRACTION99
6.8998-7.43080.32841450.24912695X-RAY DIFFRACTION96
7.4308-8.17560.25441040.23162810X-RAY DIFFRACTION99
8.1756-9.35160.22261470.18952774X-RAY DIFFRACTION99
9.3516-11.75540.18691360.15572781X-RAY DIFFRACTION98
11.7554-49.21960.21051640.14262657X-RAY DIFFRACTION95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.1867-0.3390.24780.5451-0.35160.2025-2.2285-1.1125-0.96650.00211.60190.5335-0.53491.49490.00312.98380.66-0.02152.2572-0.31441.895177.969633.270357.923
20.3904-0.7139-0.94970.64251.36872.4756-2.27271.55690.50591.20920.2472-0.63264.05830.2348-0.21733.4119-0.3018-0.46912.7416-0.01731.828178.167142.50555.9576
3-1.0487-0.2119-0.35912.9713-2.17731.66280.92540.11490.5357-0.3411-0.4626-0.5381-1.6959-1.92350.00011.7194-0.2630.16712.26120.28951.683574.789981.66671.7161
41.0420.2221.40933.3580.84141.5879-1.48411.2063-0.8805-0.4285-0.3340.1778-2.3742-1.025-0.00291.53640.1459-0.14561.98540.06581.84671.363989.897974.7114
51.8368-1.3084-1.70261.0217-0.2923.76080.1151-0.28490.2487-0.1767-0.5362-0.0778-1.2365-0.53040.00041.86190.2456-0.01011.8727-0.07651.689365.59686.245965.6119
60.8831-0.15110.24040.618-0.48770.4009-0.3555-0.9215-0.37280.7019-0.0551-0.63131.4327-0.54030.00321.9047-0.031-0.03482.0930.12451.72981.023380.432580.2491
70.29670.3468-0.91780.2425-0.83631.2749-0.4425-1.81530.7806-0.293-0.4914-0.6343-0.2415-0.29390.00191.66360.1420.00232.40260.14182.177880.499789.794888.7067
82.6029-1.88420.91020.718-0.10030.9134-0.4583-0.0294-0.3559-0.16390.2912-0.71332.2522.3907-0.00111.69330.00460.59031.99340.26172.118390.903688.326864.1742
91.4731.03860.86190.8642-0.2441.44160.10431.4823-0.8019-1.2286-0.15681.2842-1.7861-2.1466-0.00162.6960.13910.07632.79030.09271.871686.764672.410950.012
103.29323.10720.66264.12442.43062.2399-0.23650.90780.5494-0.989-0.4082-0.1038-2.6931-1.1071-0.00062.82920.54950.19872.67140.12272.203471.8501112.984962.6537
113.45491.339-0.45365.64271.59991.7564-0.2549-0.2494-0.15480.12170.1557-0.3394-0.57260.0637-0.00021.7948-0.1733-0.04091.94210.12021.877383.1361108.158186.2009
120.6115-0.56410.46291.2058-1.41593.53470.6151-1.31491.17510.1213-0.0441-0.3651-1.8709-0.7068-0.00062.01810.0843-0.1762.19090.01912.453668.3547109.453175.4279
13-0.75440.847502.76951.67433.1705-0.3446-0.32390.81780.43391.2917-2.2592-0.1820.60650.00072.0812-0.33160.39892.56260.11722.468298.87188.750657.0871
140.95190.9021-1.58880.85690.9936.6995-0.71540.20120.2829-0.00320.136-0.17550.8197-0.3101-0.00082.1273-0.0287-0.08521.80170.10752.077889.485638.3115123.8697
150.4634-1.6722-1.7632.9249-2.19862.5728-0.05370.2653-0.29490.3641-0.10860.10550.74460.11250.00042.198-0.2435-0.05221.6615-0.08791.964292.150547.9482112.4422
161.08061.0259-0.97065.4396-3.54642.1094-0.5806-0.60620.21950.1960.50691.6847-0.5107-0.35310.00043.0340.4158-0.3782.2183-0.13842.061394.695162.8333131.393
170.49970.5269-1.9242-0.1175-1.14190.671-0.0304-0.5632-0.09490.47280.25140.34030.14060.266-0.00062.35160.1073-0.09182.3607-0.03982.1242103.109153.6129135.7773
180.1204-1.49921.27352.1912-0.98110.45320.2523-0.12890.61750.5796-0.50310.2098-1.71111.33650.00032.0642-0.2393-0.35922.24810.23222.2841103.359267.7118104.6119
192.6283-0.1089-0.4591-0.1695-0.3841.19730.5679-0.35280.99620.0018-0.7389-0.19710.24760.4146-0.00051.55220.2043-0.12072.06530.00141.877896.728273.637185.2123
203.1133-2.6926-0.29226.0932-0.5028-0.30390.3952-0.9009-0.69850.1413-0.10560.04191.38270.0074-0.00121.8240.063-0.00981.8520.02671.884973.309237.954289.3753
211.61550.5449-2.46372.33760.94155.79460.01460.64260.7517-1.0960.051-0.53180.9756-0.13690.00012.30980.2064-0.13591.8307-0.01431.892172.606736.272872.0493
223.25840.1157-2.5971.4994-0.61884.66870.4973-0.76070.42670.5556-1.24090.56510.25081.31750.00031.76050.28820.06472.44680.29491.9488102.680844.651584.3019
231.8450.48270.1207-0.39530.397-0.1505-0.50410.5129-0.22231.64390.7784-0.0819-0.52470.66830.00192.2701-0.5639-0.27962.82690.42032.676779.327811.000398.5421
24-0.9260.2233-0.43021.69971.61012.4214-0.0566-0.2614-0.33910.26210.0513-0.3555-0.1358-0.11860.00022.9535-0.1835-0.2892.1314-0.10392.219365.08514.89775.5494
251.7016-0.17721.69290.47190.03341.39480.6063-0.4828-2.57261.2597-0.57790.07610.2782-0.0230.00242.4817-0.5106-0.37991.84720.03592.372564.272521.833891.7264
262.51071.62612.20231.420.28340.3852-0.0857-0.03780.0964-0.3215-0.392-0.0299-0.07870.4421-0.00012.32110.57490.10312.31640.00112.3896101.115129.655372.8129
272.6824-2.47920.48791.3393-0.5326-0.07580.10831.22020.2157-1.3799-0.7342-2.2407-0.8580.07130.00642.436-0.05860.18642.2985-0.17992.498963.43590.802539.1017
283.41571.05842.99683.76694.0084.3506-0.5923-0.43270.93640.0544-0.0469-0.550.02610.07480.00042.04980.0922-0.1222.24230.10692.004237.258981.31948.5802
290.8329-0.739-0.29280.75520.87081.1880.41840.12380.27331.1937-1.09650.0796-0.85661.22520.00222.57250.1138-0.31672.35440.052.72445.908992.89636.2042
307.1753-4.38790.68722.6209-1.87642.1298-0.0510.23210.11640.0460.38640.8983-0.0749-0.2003-0.00071.7833-0.09110.09231.9734-0.12021.834862.166265.411154.4244
313.8886-0.2254-0.42632.66960.2172.92930.33990.6145-0.0225-0.4913-0.1603-0.2325-0.07040.49502.20430.0576-0.25082.22030.20831.878342.493968.607526.9681
321.7164-0.00660.8258-2.9282-1.05212.2606-0.04780.5294-0.8416-0.14780.07960.6740.3960.5942-0.00012.30460.0026-0.2412.0825-0.09792.203852.994859.745637.2133
330.30770.7694-0.41220.9238-0.50770.0922-0.51620.71830.9009-0.251.34790.24540.66971.3781-0.00493.44260.08070.30614.19460.30382.644597.364145.852638.7407
341.8183-0.6460.4403-0.55420.06420.5427-1.5232-1.83790.62792.01791.3861-1.85421.11384.67010.00712.74670.15520.2893.4425-0.14582.343184.713852.302268.4643
350.55260.52060.28770.9882-0.11930.22712.3191-0.80980.93881.8321-1.75483.3433-1.4659-2.67310.01213.0052-0.080.18412.6538-0.33581.890961.853357.954483.6154
36-0.02780.01770.04270.0099-0.0271-0.01751.3731-0.4375-1.22230.9115-1.85172.7283-1.0217-3.52890.01012.3496-0.1505-0.0132.728-0.12982.199355.831678.085981.2371
370.89511.1479-0.25430.5545-0.6164-0.052-0.2644-2.7594-4.5142-1.26951.66680.79630.4909-2.5044-0.00892.42080.0252-0.44684.39371.12943.954529.782381.557372.3766
381.7124-2.35131.23691.978-1.47910.59030.5110.47280.8585-0.8703-0.3263-0.105-0.75150.2506-0.00081.7191-0.24170.2852.40570.08832.054284.113348.282478.1677
390.86191.0373-0.12591.1366-0.21320.0606-0.5155-2.98562.1255-2.31272.269-0.67124.1829-0.5082-04.16560.75270.08774.23610.56952.236895.577551.221352.3856
400.7577-0.37270.2176-0.0829-0.16260.0203-2.89562.5814-2.8403-2.40120.95-2.5588-0.7072-0.4199-0.00062.7797-0.92750.41414.8313-0.14333.485796.388244.216933.6865
41-0.00560.08470.01620.24390.168-0.3327-1.49560.73965.5614-0.79540.70962.28872.8538-1.0532-0.0112.14740.0040.01753.1296-0.30552.921949.040776.023576.0429
421.895-0.761-0.5109-1.5418-0.01381.502-1.57311.9461-1.0774-0.7310.91520.3493-0.09251.5445-0.00172.5709-0.4802-0.09313.10120.0452.5176102.438570.158575.2745
430.27060.88130.23911.91330.83840.03420.424-1.82551.82550.4587-0.89941.82140.14980.3378-0.00112.11880.1643-0.02662.46770.0122.715663.294462.247788.2175
440.02781.11550.52661.20631.4131.04190.0378-1.94231.03550.535-0.22160.7155-0.5398-1.56850.00172.8662-0.16840.32123.7033-0.13352.195158.403546.4145120.3695
450.65630.2219-0.4820.4405-0.47050.5654-0.6888-2.7597-0.7396-1.19050.1943.02243.27820.10070.00812.28920.206-0.11283.0674-0.1312.42772.884173.542162.434
460.3494-0.1012-0.42050.16090.00840.57191.11210.73910.4191.2420.5057-3.1959-4.07531.16160.01621.68570.20130.10532.04160.1512.387391.686573.722576.7177
470.0085-0.0656-0.12640.05720.18480.34541.41331.17241.0107-0.0572-1.6467-2.078-2.05150.43720.00322.85050.9621-0.18754.3245-0.37393.0306108.982568.128672.9445
481.9904-3.08537.48181.4699-2.22745.0689-1.9892.35763.0266-1.1198-1.1556-1.7766-2.1188-2.6885-0.2183.1918-1.20230.61795.24630.41192.6641123.056878.297575.4894
490.88620.68691.26871.11621.522.1385-2.94650.87830.85781.47040.33441.58431.0371-2.5685-0.01232.66380.2642-0.01422.7222-0.27232.281761.655552.3902103.5575
500.384-0.3997-0.22730.2780.3322-0.0173-1.9292.14741.9266-1.9194-1.2513-0.3850.67171.77160.0213.84570.50430.26545.03930.0823.904223.408480.224470.8763
510.2282-0.15630.38260.08710.42090.7105-0.54152.4931-0.0974-1.5261-1.52690.9087-4.5058-1.71620.00232.9693-0.268-0.00444.50820.32782.694657.261646.1331128.7535
520.06580.020.09650.180.19010.18741.31320.5422-0.5601-3.1511-0.9044-0.78141.36781.179-0.00011.3382-0.28550.09391.61790.10072.087751.000110.022193.3212
530.67770.60850.3340.54960.29060.157-1.19021.39380.1763-1.8081-0.55521.8048-2.0627-0.3517-0.02231.69390.37770.66041.70090.02352.108261.8136120.315289.4851
541.34930.1127-0.2220.68270.24880.7020.00951.30470.25680.0720.12970.14050.05490.44180.70482.763-0.02912.22142.79090.50212.322164.547698.23592.2823
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 1:23 )A1 - 23
2X-RAY DIFFRACTION2( CHAIN A AND RESID 24:45 )A24 - 45
3X-RAY DIFFRACTION3( CHAIN A AND RESID 46:75 )A46 - 75
4X-RAY DIFFRACTION4( CHAIN A AND RESID 76:111 )A76 - 111
5X-RAY DIFFRACTION5( CHAIN A AND RESID 112:152 )A112 - 152
6X-RAY DIFFRACTION6( CHAIN A AND RESID 153:173 )A153 - 173
7X-RAY DIFFRACTION7( CHAIN A AND RESID 174:197 )A174 - 197
8X-RAY DIFFRACTION8( CHAIN A AND RESID 198:223 )A198 - 223
9X-RAY DIFFRACTION9( CHAIN A AND RESID 224:269 )A224 - 269
10X-RAY DIFFRACTION10( CHAIN B AND RESID 1:67 )B1 - 67
11X-RAY DIFFRACTION11( CHAIN B AND RESID 68:154 )B68 - 154
12X-RAY DIFFRACTION12( CHAIN B AND RESID 155:197 )B155 - 197
13X-RAY DIFFRACTION13( CHAIN B AND RESID 198:269 )B198 - 269
14X-RAY DIFFRACTION14( CHAIN C AND RESID 1:154 )C1 - 154
15X-RAY DIFFRACTION15( CHAIN C AND RESID 155:269 )C155 - 269
16X-RAY DIFFRACTION16( CHAIN D AND RESID 1:97 )D1 - 97
17X-RAY DIFFRACTION17( CHAIN D AND RESID 98:197 )D98 - 197
18X-RAY DIFFRACTION18( CHAIN D AND RESID 198:269 )D198 - 269
19X-RAY DIFFRACTION19( CHAIN E AND RESID 1:60 )E1 - 60
20X-RAY DIFFRACTION20( CHAIN E AND ( RESID 61:154 OR RESID 155:155 ) )E61 - 154
21X-RAY DIFFRACTION20( CHAIN E AND ( RESID 61:154 OR RESID 155:155 ) )E155
22X-RAY DIFFRACTION21( CHAIN E AND RESID 156:213 )E156 - 213
23X-RAY DIFFRACTION22( CHAIN E AND RESID 214:269 )E214 - 269
24X-RAY DIFFRACTION23( CHAIN F AND RESID 1:59 )F1 - 59
25X-RAY DIFFRACTION24( CHAIN F AND RESID 60:174 )F60 - 174
26X-RAY DIFFRACTION25( CHAIN F AND RESID 175:197 )F175 - 197
27X-RAY DIFFRACTION26( CHAIN F AND RESID 198:269 )F198 - 269
28X-RAY DIFFRACTION27( CHAIN G AND RESID 1:59 )G1 - 59
29X-RAY DIFFRACTION28( CHAIN G AND RESID 60:156 )G60 - 156
30X-RAY DIFFRACTION29( CHAIN G AND RESID 157:197 )G157 - 197
31X-RAY DIFFRACTION30( CHAIN G AND RESID 198:269 )G198 - 269
32X-RAY DIFFRACTION31( CHAIN H AND RESID 1:154 )H1 - 154
33X-RAY DIFFRACTION32( CHAIN H AND RESID 155:269 )H155 - 269
34X-RAY DIFFRACTION33( CHAIN I AND RESID 1:10 )I1 - 10
35X-RAY DIFFRACTION34( CHAIN I AND RESID 11:20 )I11 - 20
36X-RAY DIFFRACTION35( CHAIN I AND RESID 21:25 )I21 - 25
37X-RAY DIFFRACTION36( CHAIN I AND RESID 26:30 )I26 - 30
38X-RAY DIFFRACTION37( CHAIN I AND RESID 31:42 )I31 - 42
39X-RAY DIFFRACTION38( CHAIN J AND RESID 1:10 )J1 - 10
40X-RAY DIFFRACTION39( CHAIN J AND RESID 11:15 )J11 - 15
41X-RAY DIFFRACTION40( CHAIN J AND RESID 16:22 )J16 - 22
42X-RAY DIFFRACTION41( CHAIN K AND RESID 9:16 )K9 - 16
43X-RAY DIFFRACTION42( CHAIN L AND RESID 5:19 )L5 - 19
44X-RAY DIFFRACTION43( CHAIN L AND RESID 20:29 )L20 - 29
45X-RAY DIFFRACTION44( CHAIN L AND RESID 30:42 )L30 - 42
46X-RAY DIFFRACTION45( CHAIN M AND RESID 1:5 )M1 - 5
47X-RAY DIFFRACTION46( CHAIN M AND RESID 6:10 )M6 - 10
48X-RAY DIFFRACTION47( CHAIN M AND RESID 11:15 )M11 - 15
49X-RAY DIFFRACTION48( CHAIN M AND RESID 16:18 )M16 - 18
50X-RAY DIFFRACTION49( CHAIN N AND RESID 9:16 )N9 - 16
51X-RAY DIFFRACTION50( CHAIN k AND RESID 1:8 )k1 - 8
52X-RAY DIFFRACTION51( CHAIN n AND RESID 1:8 )n1 - 8
53X-RAY DIFFRACTION52( CHAIN G AND RESID 301:301 ) OR ( CHAIN F AND RESID 302:312 )G301
54X-RAY DIFFRACTION52( CHAIN G AND RESID 301:301 ) OR ( CHAIN F AND RESID 302:312 )F302 - 312
55X-RAY DIFFRACTION53( CHAIN B AND RESID 302:313 )B302 - 313
56X-RAY DIFFRACTION54( CHAIN A AND RESID 302:305 ) OR ( CHAIN B AND RESID 314:321 )A302 - 305
57X-RAY DIFFRACTION54( CHAIN A AND RESID 302:305 ) OR ( CHAIN B AND RESID 314:321 )B314 - 321

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