+Open data
-Basic information
Entry | Database: PDB / ID: 5ei0 | |||||||||
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Title | Structure of RCL-cleaved vaspin (serpinA12) | |||||||||
Components | Serpin A12 | |||||||||
Keywords | HYDROLASE INHIBITOR / Serpin / cleaved / adipokine | |||||||||
Function / homology | Function and homology information regulation of triglyceride metabolic process / negative regulation of lipid biosynthetic process / lipid biosynthetic process / regulation of cholesterol metabolic process / molecular function inhibitor activity / negative regulation of gluconeogenesis / positive regulation of insulin receptor signaling pathway / phosphatidylinositol 3-kinase/protein kinase B signal transduction / gluconeogenesis / serine-type endopeptidase inhibitor activity ...regulation of triglyceride metabolic process / negative regulation of lipid biosynthetic process / lipid biosynthetic process / regulation of cholesterol metabolic process / molecular function inhibitor activity / negative regulation of gluconeogenesis / positive regulation of insulin receptor signaling pathway / phosphatidylinositol 3-kinase/protein kinase B signal transduction / gluconeogenesis / serine-type endopeptidase inhibitor activity / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / extracellular space / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | |||||||||
Authors | Pippel, J. / Kuettner, B.E. / Ulbricht, D. / Daberger, J. / Schultz, S. / Heiker, J.T. / Strater, N. | |||||||||
Funding support | Germany, 2items
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Citation | Journal: Biol.Chem. / Year: 2016 Title: Crystal structure of cleaved vaspin (serpinA12). Authors: Pippel, J. / Kuettner, E.B. / Ulbricht, D. / Daberger, J. / Schultz, S. / Heiker, J.T. / Strater, N. #1: Journal: Cell. Mol. Life Sci. / Year: 2013 Title: Vaspin inhibits kallikrein 7 by serpin mechanism. Authors: Heiker, J.T. / Kloeting, N. / Kovacs, P. / Kuettner, E.B. / Strater, N. / Schultz, S. / Kern, M. / Stumvoll, M. / Blueher, M. / Beck-Sickinger, A.G. #2: Journal: Biochem. J. / Year: 2015 Title: A unique serpin P1' glutamate and a conserved beta-sheet C arginine are key residues for activity, protease recognition and stability of serpinA12 (vaspin). Authors: Ulbricht, D. / Pippel, J. / Schultz, S. / Meier, R. / Strater, N. / Heiker, J.T. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5ei0.cif.gz | 293 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5ei0.ent.gz | 241.4 KB | Display | PDB format |
PDBx/mmJSON format | 5ei0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5ei0_validation.pdf.gz | 434.4 KB | Display | wwPDB validaton report |
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Full document | 5ei0_full_validation.pdf.gz | 437.4 KB | Display | |
Data in XML | 5ei0_validation.xml.gz | 25.8 KB | Display | |
Data in CIF | 5ei0_validation.cif.gz | 34.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ei/5ei0 ftp://data.pdbj.org/pub/pdb/validation_reports/ei/5ei0 | HTTPS FTP |
-Related structure data
Related structure data | 4if8S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 47536.812 Da / Num. of mol.: 2 / Fragment: UNP residues 22-414 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SERPINA12 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q8IW75 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.67 % |
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Crystal grow | Temperature: 295.15 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 20 % (w/v) PEG 3350, 20 % (v/v) isopropanol, 0.1 M imidazole-HCl pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.91841 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Mar 11, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91841 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→29.19 Å / Num. obs: 23966 / % possible obs: 89.2 % / Redundancy: 4.2 % / Biso Wilson estimate: 42.8 Å2 / Rmerge(I) obs: 0.165 / Net I/σ(I): 7 |
Reflection shell | Resolution: 2.5→2.6 Å / Redundancy: 4.1 % / Rmerge(I) obs: 1.659 / Mean I/σ(I) obs: 0.9 / % possible all: 57.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4IF8 Resolution: 2.5→29.189 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 35.3 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 55.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→29.189 Å
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Refine LS restraints |
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LS refinement shell |
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