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- PDB-5e1l: Structural and functional analysis of the E. coli FtsZ interactin... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5e1l | ||||||
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Title | Structural and functional analysis of the E. coli FtsZ interacting protein, ZapC, reveals insight into molecular properties of a novel Z ring stabilizing protein | ||||||
![]() | Cell division protein ZapC | ||||||
![]() | CELL CYCLE / Z ring / cell division | ||||||
Function / homology | ![]() : / FtsZ-dependent cytokinesis / division septum assembly / cell division site / regulation of cell division / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Schumacher, M.A. / Huang, K.-H. / Tchorzewski, L. / Zeng, W. / Janakiraman, A. | ||||||
![]() | ![]() Title: Structural and Functional Analyses Reveal Insights into the Molecular Properties of the Escherichia coli Z Ring Stabilizing Protein, ZapC. Authors: Schumacher, M.A. / Zeng, W. / Huang, K.H. / Tchorzewski, L. / Janakiraman, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51 KB | Display | ![]() |
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PDB format | ![]() | 35.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22783.135 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: ycbW, zapC, AC789_1c09860, BN1008_2209, ECONIH1_05985, EL75_2796, EL79_2872, EL80_2810, HUS2011_1029, HW43_08455, IY32_15190, PD07_27680, PGD_02344, PU06_12140, PU21_19080, PU69_14370, RR31_ ...Gene: ycbW, zapC, AC789_1c09860, BN1008_2209, ECONIH1_05985, EL75_2796, EL79_2872, EL80_2810, HUS2011_1029, HW43_08455, IY32_15190, PD07_27680, PGD_02344, PU06_12140, PU21_19080, PU69_14370, RR31_05140, UN86_22105, UN92_26695, WQ64_03785, WQ65_11505, WQ66_20855, WQ69_14055, WQ70_10055, WQ71_17640, WQ72_12045, WQ73_04655, WQ74_18750, WQ77_23190, WQ79_17420, WQ84_15610, WQ86_15290, WQ88_01710, WQ92_17480, WQ95_04390, WQ99_00770, WR00_01300, WR01_18315, WR03_21275, WR05_24825, WR12_12790, WR13_00430, WR16_17045, WR17_22160, WR18_05905, WR19_22835, WR23_12280, WR24_08325, XB00_15425 Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.01 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 1.5 M sodium citrate, 0.1 M cacodylate pH 6.5 / PH range: 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 21, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→60.5 Å / Num. obs: 9961 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3 % / Rsym value: 0.02 / Net I/σ(I): 11.5 |
Reflection shell | Rmerge(I) obs: 0.234 / Mean I/σ(I) obs: 4.6 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 40.377 Å2 / ksol: 0.344 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 80.85 Å2 / Biso mean: 27.4791 Å2 / Biso min: 8.67 Å2
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Refinement step | Cycle: final / Resolution: 2.15→37.643 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 7
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