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Open data
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Basic information
Entry | Database: PDB / ID: 5dp1 | ||||||
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Title | Crystal structure of CurK enoyl reductase | ||||||
![]() | CurK | ||||||
![]() | OXIDOREDUCTASE / Polyketide synthase / enoyl reductase | ||||||
Function / homology | ![]() DIM/DIP cell wall layer assembly / fatty acid synthase activity / secondary metabolite biosynthetic process / phosphopantetheine binding / fatty acid biosynthetic process / oxidoreductase activity / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Khare, D. / Smith, J.L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase. Authors: Khare, D. / Hale, W.A. / Tripathi, A. / Gu, L. / Sherman, D.H. / Gerwick, W.H. / Hakansson, K. / Smith, J.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 150.2 KB | Display | ![]() |
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PDB format | ![]() | 117.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.1 KB | Display | ![]() |
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Full document | ![]() | 451.1 KB | Display | |
Data in XML | ![]() | 16.8 KB | Display | |
Data in CIF | ![]() | 24.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5dovC ![]() 5dozC ![]() 5dp2C ![]() 2vz9S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 39471.758 Da / Num. of mol.: 1 / Fragment: UNP residues 1482-1815 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: F4Y425 | ||||
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#2: Chemical | ChemComp-GOL / #3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.9 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop Details: 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.2 M Li2SO4 and 0.1 M CAPS pH 10.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 19, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→24.3 Å / Num. obs: 32609 / % possible obs: 99 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.059 / Net I/σ(I): 21 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2VZ9 Resolution: 1.85→24.3 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.944 / SU B: 6.738 / SU ML: 0.099 / Cross valid method: THROUGHOUT / ESU R: 0.136 / ESU R Free: 0.128 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.418 Å2
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Refinement step | Cycle: 1 / Resolution: 1.85→24.3 Å
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Refine LS restraints |
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